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Cloning °ü·Ã
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In-Fusion Cloning
> [Kit] In-Fusion¢ç Cloning
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
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Clontech
639649
In-Fusion¢ç HD Cloning Kit
50 ȸ
1,409,000¿ø
Àç°í¼ÒÁø ÈÄ Á¾¸Å
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Clontech
639650
In-Fusion¢ç HD Cloning Kit
100 ȸ
2,439,000¿ø
Àç°í¼ÒÁø ÈÄ Á¾¸Å
Clontech
639615
Cloning Enhancer
100 ȸ
393,000¿ø
Clontech
636763
Stellar¢â Competent Cells
10 ȸ (10 x 100ul)
293,000¿ø
Clontech
636767
Stellar¢â Competent Cells (96 well plate)
96 ȸ (96 x 20ul)
(Package)
822,000¿ø
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[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
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[NEW] In-Fusion¢ç Snap..
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[Kit] In-Fusion¢ç Clon..
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Vector (Cloning ¿ë)
M13 mp18 Single Strand..
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pUC118/119 type vector
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¡Ø In-Fusion Cloning »ý»ý Èı⺸±â
*In-Fusion® HD Cloning Kit ´Â
In-Fusion® Snap Assembly Master Mix
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PCR Cloning Kits¡ªDNA Cloning¡ªIn Fusion HD Cloning System
In-Fusion Cloning SystemÀº ¾î¶² PCR ´ÜÆíÀ̳ª º¹¼öÀÇ DNA ´ÜÆíµµ ¼±ÇüÈµÈ vector(linearized vector)¿¡ 15ºÐ(In-Fusion HD EcoDry´Â 30ºÐ)ÀÇ ¹ÝÀÀÀ¸·Î directional cloningÀÌ °¡´ÉÇÏ´Ù (Figure 1). ¶ÇÇÑ PCR ´ÜÆíÀº Á¦ÇÑÈ¿¼Ò󸮳ª phosphorylation°ú °°Àº Ãß°¡ÀûÀΠó¸® ´Ü°è°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù. In-Fusion È¿¼Ò´Â ¼±ÇüÈµÈ vector ¸»´Ü°ú PCR·Î ÁõÆøµÈ PCR ´ÜÆí(insert)ÀÇ ¸»´ÜÀÇ 15bp homologue sequence¸¦ ÀνÄÇÏ¿© À¶ÇÕ½ÃŲ´Ù. ÀÌ 15 bpÀÇ À¶ÇÕ¼¿Àº ¿øÇÏ´Â sequence¸¦ ÁõÆøÇÏ°íÀÚ ÇÏ´Â PCR primer¿¡ µðÀÚÀÎÇÏ¿© ²¸ ³ÖÀ¸¸é ½±°Ô ¸¸µé ¼ö ÀÖ´Ù.
ÀÌ ½ÇÇè¹æ¹ýÀ» »ç¿ëÇÏ¸é ¾Æ·¡¿Í °°Àº ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
Subcloning ¾øÀ̵µ º¹¼öÀÇ DNA ´ÜÆíÀ» ¿øÇÏ´Â vector¿¡ Cloning
³»ºÎÀÇ Æ¯Á¤ ºÎºÐ¸¸ ±³Ã¼ÇÏ´Â Modular expression vector Á¦ÀÛ
ºÒÇÊ¿äÇÑ ¿°±â°¡ ¾ø´Â Fusion protein ±¸Ãà
DNA ´ÜÆíÀÇ Á¦°Å³ª ±³Ã¼
InsertÀÇ point mutationÀ̳ª Á¦ÇÑÈ¿¼Ò »çÀÌÆ® ÷°¡
À¯ÀüÀÚ³» Çü±¤ ´Ü¹éÁúÀÇ tag ±³Ã¼
cDNA·Î UTRs÷°¡
Figure 1. The In-Fusion cloning protocol.
Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments.
Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).
Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction.
Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.
Citations
A highly photostable and bright green fluorescent protein
Nature Biotechnology | 2022 Apr 25 | PubMed ID: 35468954 |
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Distinct modes of mitochondrial metabolism uncouple T helper cell differentiation and function
Nature | 2019 Jul 05 | PubMed ID: 31217581 |
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Saturation Editing of Genomic Regions by Multiplex Homology-Directed Repair
Nature | 2014 Sep 04 | PubMed ID: 25141179 |
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Endocrine Deficiency As a Function of Radiation Dose to the Hypothalamus and Pituitary in Pediatric and Young Adult Patients With Brain Tumors
Journal of Clinical Oncology | 2018 Oct 01 | PubMed ID: 30118397 |
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SPARC enables genetic manipulation of precise proportions of cells.
Nature neuroscience | 2020 Jul 20 | PubMed ID: 32690967 |
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SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Cell Stem Cell | 2020 Oct 13 | PubMed ID: 33113348 |
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Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission
Cell Host & Microbe | 2022 Jan 21 | PubMed ID: 35150638 |
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Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity
Molecular cell | 2022 Jun 08 | PubMed ID: 35219382 |
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The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Molecular cell | 2019 Jan 10 | PubMed ID: 30639242 |
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Identification of a novel deFADding activity in human, yeast and bacterial 5′ to 3′ exoribonucleases
Nucleic Acids Research | 2022 Aug 26 | PubMed ID: 35904778 |
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Experimental demonstration of operon formation catalyzed by insertion sequence
Nucleic Acids Research | 2022 Feb 22 | PubMed ID: 35066585 |
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Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes
Nucleic Acids Research | 2021 Sep 07 | PubMed ID: 33907814 |
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Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P
Nucleic Acids Research | 2022 Mar 21 | PubMed ID: 35188576 |
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Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
Nucleic Acids Research | 2019 Jul 26 | PubMed ID: 31334814 |
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An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation
Nature Plants | 2021 Nov 08 | PubMed ID: 34750500 |
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NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart
Nature Communications | 2022 Oct 20 |
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Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Nature Communications | 2022 Sep 23 | PubMed ID: 36151095 |
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Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR
Nature Communications | 2020 Sep 16 | PubMed ID: 32938929 |
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Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
Nature Communications | 2020 Aug 18 | PubMed ID: 32811819 |
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The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Nature Communications | 2022 Jul 15 | PubMed ID: 35840578 |
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Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater
Nature Communications | 2022 Feb 03 | PubMed ID: 35115523 |
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ICER is requisite for Th17 differentiation
Nature Communications | 2016 Sep 29 | PubMed ID: 27680869 |
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Therapeutic homology-independent targeted integration in retina and liver
Nature Communications | 2022 Apr 12 | PubMed ID: 35414130 |
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Robust and tunable signal processing in mammalian cells via engineered covalent modification cycles
Nature Communications | 2022 Mar 31 | PubMed ID: 35361767 |
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An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells
Nature Communications | 2020 Nov 10 |
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Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases
Nature Communications | 2022 Jul 29 | PubMed ID: 35906209 |
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A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
ACS Central Science | 2021 Jan 05 |
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Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer
Science Advances | 2020 Dec 02 | PubMed ID: 33268361 |
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Characterization of a vaccine-elicited human antibody with sequence homology to VRC01-class antibodies that binds the C1C2 gp120 domain
Science Advances | 2022 May 04 | PubMed ID: 35507661 |
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Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances | 2021 Mar 03 | PubMed ID: 33658195 |
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A two-step mechanism governing PARP1-DNA retention by PARP inhibitors
Science Advances | 2022 Sep 01 | PubMed ID: 36070389 |
Read Article
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Keyword : 639648,infusion,in fusion,cloning,Ŭ·Î´×,º¹¼ö´ÜÆí,ÀÎÇ»Àü,ÇÁ·Î¹Í½º,premix, mutagenesis, mutation,soeasy,so easy
In-Fusion Cloning
[Overview] In-Fusion¢ç Cloning
[°³¿ä] So Easy Product
Online Tools for In-Fusion
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
In-Fusion¢ç cloningÀ» ¹Ù·Î ÁøÇàÇϱâ À§ÇÑ lin..
In-Fusion¢ç Ready Vectors