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Home > 전제품보기 > Cloning 관련 > In-Fusion Cloning > [Kit] In-Fusion® Cloning
Premix화! 시간단축! 효율성 향상!

[Kit] In-Fusion® Cloning

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Clontech
 639615
 Cloning Enhancer
관련학술 구매하기 		100 회
 405,000원  제조사 페이지로 바로가기 x342553
Clontech
 636763
 Stellar™ Competent Cells
관련학술 구매하기 LMO제품  		10 회 (10 x 100ul)
 293,000원  제조사 페이지로 바로가기 x33081
Clontech
 636767
 Stellar™ Competent Cells (96 well plate)
관련학술 구매하기 LMO제품  		96 회 (96 x 20ul)
(Package) 		822,000원  제조사 페이지로 바로가기 x33079



※ In-Fusion Cloning 생생 후기보기

 *In-Fusion® HD Cloning Kit 는 In-Fusion® Snap Assembly Master Mix로 업그레이드 되었습니다.

PCR Cloning Kits―DNA Cloning―In Fusion HD Cloning System
In-Fusion Cloning System은 어떤 PCR 단편이나 복수의 DNA 단편도 선형화된 vector(linearized vector)에 15분(In-Fusion HD EcoDry는 30분)의 반응으로 directional cloning이 가능하다 (Figure 1). 또한 PCR 단편은 제한효소처리나 phosphorylation과 같은 추가적인 처리 단계가 필요하지 않다. In-Fusion 효소는 선형화된 vector 말단과 PCR로 증폭된 PCR 단편(insert)의 말단의 15bp homologue sequence를 인식하여 융합시킨다. 이 15 bp의 융합서열은 원하는 sequence를 증폭하고자 하는 PCR primer에 디자인하여 껴 넣으면 쉽게 만들 수 있다.

이 실험방법을 사용하면 아래와 같은 다양한 실험에 적용 가능하다.
  • Subcloning 없이도 복수의 DNA 단편을 원하는 vector에 Cloning
  • 내부의 특정 부분만 교체하는 Modular expression vector 제작
  • 불필요한 염기가 없는 Fusion protein 구축
  • DNA 단편의 제거나 교체
  • Insert의 point mutation이나 제한효소 사이트 첨가
  • 유전자내 형광 단백질의 tag 교체
  • cDNA로 UTRs첨가


Figure 1. The In-Fusion cloning protocol.
Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).


Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.
Citations
A highly photostable and bright green fluorescent protein
 Nature Biotechnology    |    2022 Apr 25    |    PubMed ID: 35468954    |    Read Article

Distinct modes of mitochondrial metabolism uncouple T helper cell differentiation and function
Nature    |    2019 Jul 05    |    PubMed ID: 31217581    |    Read Article

Saturation Editing of Genomic Regions by Multiplex Homology-Directed Repair
Nature    |    2014 Sep 04    |    PubMed ID: 25141179    |    Read Article

Endocrine Deficiency As a Function of Radiation Dose to the Hypothalamus and Pituitary in Pediatric and Young Adult Patients With Brain Tumors
Journal of Clinical Oncology    |    2018 Oct 01    |    PubMed ID: 30118397    |    Read Article

SPARC enables genetic manipulation of precise proportions of cells.
Nature neuroscience    |    2020 Jul 20    |    PubMed ID: 32690967    |    Read Article

SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Cell Stem Cell    |    2020 Oct 13    |    PubMed ID: 33113348    |    Read Article

Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission
Cell Host & Microbe    |    2022 Jan 21    |    PubMed ID: 35150638    |    Read Article

Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity
Molecular cell    |    2022 Jun 08    |    PubMed ID: 35219382    |    Read Article

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Molecular cell    |    2019 Jan 10    |    PubMed ID: 30639242    |    Read Article

Identification of a novel deFADding activity in human, yeast and bacterial 5′ to 3′ exoribonucleases
Nucleic Acids Research    |    2022 Aug 26    |    PubMed ID: 35904778    |    Read Article

Experimental demonstration of operon formation catalyzed by insertion sequence
Nucleic Acids Research    |    2022 Feb 22    |    PubMed ID: 35066585    |    Read Article

Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes
Nucleic Acids Research    |    2021 Sep 07    |    PubMed ID: 33907814    |    Read Article

 Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P
 Nucleic Acids Research    |    2022 Mar 21    |    PubMed ID: 35188576    |    Read Article

Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
Nucleic Acids Research    |    2019 Jul 26    |    PubMed ID: 31334814    |    Read Article

An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation
Nature Plants    |    2021 Nov 08    |    PubMed ID: 34750500    |    Read Article

NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart
Nature Communications    |    2022 Oct 20    |    Read Article

Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Nature Communications    |    2022 Sep 23    |    PubMed ID: 36151095    |    Read Article

 Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR
Nature Communications    |    2020 Sep 16    |    PubMed ID: 32938929    |    Read Article

Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
Nature Communications    |    2020 Aug 18    |    PubMed ID: 32811819    |    Read Article

The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Nature Communications    |    2022 Jul 15    |    PubMed ID: 35840578    |    Read Article

Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater
Nature Communications    |    2022 Feb 03    |    PubMed ID: 35115523    |    Read Article

ICER is requisite for Th17 differentiation
Nature Communications    |    2016 Sep 29    |    PubMed ID: 27680869    |    Read Article

Therapeutic homology-independent targeted integration in retina and liver
Nature Communications    |    2022 Apr 12    |    PubMed ID: 35414130    |    Read Article

Robust and tunable signal processing in mammalian cells via engineered covalent modification cycles
Nature Communications    |    2022 Mar 31    |    PubMed ID: 35361767    |    Read Article

An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells
Nature Communications    |    2020 Nov 10    |    Read Article

Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases
Nature Communications    |    2022 Jul 29    |    PubMed ID: 35906209    |    Read Article

A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
ACS Central Science    |    2021 Jan 05    |    Read Article

Regulatory encoding of quantitative variation in spatial activity of a Drosophila enhancer
Science Advances    |    2020 Dec 02    |    PubMed ID: 33268361    |    Read Article

Characterization of a vaccine-elicited human antibody with sequence homology to VRC01-class antibodies that binds the C1C2 gp120 domain
Science Advances    |    2022 May 04    |    PubMed ID: 35507661    |    Read Article

 Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances    |    2021 Mar 03    |    PubMed ID: 33658195    |    Read Article

A two-step mechanism governing PARP1-DNA retention by PARP inhibitors
Science Advances    |    2022 Sep 01    |    PubMed ID: 36070389    |    Read Article


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In-Fusion Cloning
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최고의 Colony 형성능과 정확한 PCR cloning
[NEW] In-Fusion® Snap Assembly
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Premix화! 시간단축! 효율성 향상!
[Kit] In-Fusion® Cloning
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In-Fusion® cloning을 바로 진행하기 위한 lin..
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