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Home > 전제품보기 > 세포 · 배지 > Bioassay 시약 > PPiLight Inorganic Pyrophosphate Assay

PPiLight Inorganic Pyrophosphate Assay

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Lonza
LT07-610
PPiLight Inorganic Pyrophosphate Assay
관련학술 관심상품등록 구매하기
500 tests
435,000원  PPiLight_inorganic_pyrophosphate_assay.pdf

제품설명
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3) and ligases (EC 6).

The PPiLight™ Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.


Figure 1: Bioluminescent reaction
특장점
  • Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
  • Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
  • Wide Detection Range - Sensitive to 0.02 μM with a linear range to 10 μM
  • Versatile - Scalable to 96-, 384-, 1586-well formats
Applications
Measure activity of:
  • Phosphotransferases
  • Pyrophosphotases
  • Cyclases
  • Ligases
  • Hydrolases
  • DNA Polymerases

보존
2°C - 8°C

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