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제품명/제품코드
사이트내검색
Plasmid DNA와 siRNA/miRNA를 효과적으로 delivery

TransIT-X2 Dynamic Delivery System

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 6003
TransIT-X2 Dynamic Delivery System
관련학술 관심상품등록 구매하기
0.3 ml
213,000원  ml061-transit-x2-dynamic-delivery-system-user-protocol.pdf
Mirus
MIR 6004
TransIT-X2 Dynamic Delivery System
관련학술 관심상품등록 구매하기
0.75 ml
532,000원 
Mirus
MIR 6000
TransIT-X2 Dynamic Delivery System
관련학술 관심상품등록 구매하기
1.5 ml
888,000원 
Mirus
MIR 6005
TransIT-X2 Dynamic Delivery System
관련학술 관심상품등록 구매하기
1.5 ml×5
3,859,000원 
Mirus
MIR 6006
TransIT-X2 Dynamic Delivery System
관련학술 관심상품등록 구매하기
1.5 ml×10
7,097,000원 

TransIT-X2 Dynamic Delivery System은 non-liposomal polymeric system을 이용하여 primary cell을 포함한 다양한 cell type에 효과적으로 형질전환이 가능하다. TransIT-X2 Dynamic Delivery System는 DNA, siRNA의 형질전환에 이용할 수 있으며, DNA와 RNA 한꺼번에 형질전환도 가능하다. TransIT-X2 은 형질전환 후에 배지 교체가 불필요하며, serum이 포함된 배지에서 형질전환이 가능하다. TransIT-X2 Dynamic Delivery System은 아래와 같은 특징이 있다.
  • Efficiency-Exceptional Broad Spectrum Transfection
  • Versatility-Cutting Edge Delivery of Plasmid DNA and siRNA
  • Technology-Novel, Non-Liposomal, Polymeric Delivery
SPECIFICATIONS

Storage

Store TransIT-X2 Dynamic Delivery System at -20°C. Before each use, warm to room temperature and vortex gently.

Product Guarantee

1 year from the date of purchase, when properly stored and handled.



Figure 1. Visualization of High GFP Expression using TransIT-X2™ Dynamic Delivery System. TransIT-X2 Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, HepG2, LNCaP, MDCK, PC12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 35 mm MatTek dishes using 4-8 μl of TransIT-X2 to deliver 2 μg of DNA. Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

* indicates primary cell types


Figure 2.High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells using TransIT-X2™ Dynamic Delivery System. TransIT-X2 Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 96-well plates using 0.2-0.4 μl of TransIT-X2 to deliver 0.1 μg of DNA (2:1, 3:1 or 4:1 reagent: DNA ratio). Triplicate wells were assayed 48 hours post-transfection using guava easyCyte™ 5HT Flow Cytometer.

*indicates primary cell types


Figure 3. TransIT-X2™ Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000. TransIT-X2 Dynamic Delivery System and Lipofectamine 2000 Transfection Reagent were used to transfect siRNA targeting endogenous proteins - GAPDH, aha1 or non-targeting control in normal human dermal fibroblasts (NHDF). Cells were transfected in a 6-well plate using 4 μl of TransIT-X2 or 6 μl of Lipofectamine 2000 and 25 nM siRNA according to each manufacturer's protocol. The amount of GAPDH or aha1 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the non-targeting control, 48 hours post-transfection. Error bars represent the standard deviation of triplicate wells.


Figure 4. Effective miRNA Delivery using TransIT-X2™ Dynamic Delivery System Yields Decreased Levels of PTK9 mRNA. TransIT-X2 Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect Pre-miR™ hsa-miR-1 miRNA Precursor or mirVana™ miRNA mimic, miR-1,both known to decrease PTK9 mRNA levels. A Pre-miR negative control was transfected to assess baseline mRNA levels. T47D cells were transfected in a 12-well plate using 3 μl of TransIT-X2 or Lipofectamine 2000 and 50 nM miRNA according to each manufacturer's protocol. The amount of PTK9 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the negative control, 48 hours post-transfection. Error bars represent the standard deviation of triplicate wells.

Keyword : v6003,v6004,v6000,v6005,v6006,mir6003,mir6004,mir6000,mir6005,mir6006
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Plasmid DNA와 siRNA/miRNA를 효과적으로 delivery
> TransIT-X2 Dynamic Delivery System
Mammalian cell 용 고효율 · 저독성 Transfection ..
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Mous ES 세포에 최적화된 Transfection 시약
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One Tube=One Transfection format
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