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제품명/제품코드
사이트내검색

TransIT-Insect Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 6104
TransIT-Insect Transfection Reagent
관련학술 관심상품등록 구매하기
0.4 ml
183,200원 
229,000원
가격할인
09.11 ~ 10.31
transit-insect-transfection-reagent.pdf
Mirus
MIR 6100
TransIT-Insect Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml
280,000원 
476,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6105
TransIT-Insect Transfection Reagent
관련학술 관심상품등록 구매하기
5 x 1 ml
1,651,200원 
2,064,000원
가격할인
09.11 ~ 10.31
Mirus
MIR 6106
TransIT-Insect Transfection Reagent
관련학술 관심상품등록 구매하기
10 x 1 ml
3,034,400원 
3,793,000원
가격할인
09.11 ~ 10.31

특징
  • 뛰어난 DNA 도입 - Sf9, High Five™, S2를 포함한 insect cell에 도입
  • 높은 Baculovirus 바이러스 생산 - 곤충 세포에서 Baculovirus 발현에 최적
  • Serum compatibility - Media change가 필요 없는 Non-liposomal, animal-origin free formulation
  • 적은양의 reagent로 효과적인 transfection
제품 설명
TransIT®-Insect Transfection Reagent is a novel transfection formulation specifically developed for high-performance transfection of plasmid DNA into insect cells such as: Sf9, High Five™ (BTI-TN-5B1-4), and Drosophila melanogaster Schneider’s 2 (S2 or D. Mel. (2)). TransIT-Insect can also be used to transfect insect cells with baculovirus DNA to make recombinant virus. TransIT-Insect is composed of animal-origin free components and is serum compatible, which eliminates the need for any culture medium change after transfection.
SPECIFICATIONS

Storage

Store TransIT-Insect Reagent at -20°C.
Before each use, warm to room temperature and vortex gently.

Product Guarantee

6 months from the date of purchase, when properly stored and handled.

Data

그림. Efficient Transfection of Baculovirus Genomic DNA using TransIT-Insect Reagent. Transfections were performed in 6-well plates with 5 x 105 Sf9 cells per well using TransIT-Insect Transfection Reagent at the reagent-to-total DNA ratio of 3:1 (μl:μg). Cells were co-transfected with 0.5 μg of ProGreen™ baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 μg of pVL1393 transfer vector (AB Vector). (A) Fluorescence and phase contrast images were taken at 6 days post-transfection using a Zeiss S100 fluorescent microscope. Merge shown in (B).


그림. TransIT-Insect Outperforms Competitor Transfection Reagents. Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 cells were transfected in 96-well plates with 0.1 μg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (μl: μg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.

Keyword : v6104,v6100,v6103,v6105,v6106,mir6104,mir6100,mir6103,mir6105,mir6106
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