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°í¿ª°¡ÀÇ Baculvoirus »ý»êÀ» À§ÇÑ Àü¿ë½Ã¾à

TransIT¢ç-Insect Transfection Reagent

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6104
TransIT-Insect Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.4 ml
°¡°Ý¹®ÀÇ   transit-insect-transfection-reagent.pdf
Mirus
MIR 6100
TransIT-Insect Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 6105
TransIT-Insect Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 x 1 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 6106
TransIT-Insect Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 x 1 ml
°¡°Ý¹®ÀÇ  



  • Sf9, High Five¢â, S2 µî ´Ù¾çÇÑ Insect cell lineÀÇ transfection¿¡ ÃÖÀû
  • ³ôÀº È¿À²·Î Baculovirus »ý»ê °¡´É
  • Plaque assay¾øÀÌ ´Ü ±â°£¿¡ 1 step expression system ±¸Ãà ½ÇÇö
  • Serum compatibility - Media change°¡ ÇÊ¿ä ¾ø´Â Non-liposomal, animal-origin free formulation
Á¦Ç° ¼³¸í
°ïÃæ ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛÀº ¹ßÇöµÈ ´Ü¹éÁúÀÇ ¹ø¿ª ÈÄ °úÁ¤À» ±¸ÇöÇÒ ¼ö ÀÖ¾î À¯¿ëÇϸç, ÀϹÝÀûÀ¸·Î °ïÃæ¼¼Æ÷¿¡¼­ÀÇ ÀϽÃÀûÀÎ ¹ßÇö ¶Ç´Â ÀçÁ¶ÇÕ baculovirus¸¦ ÀÌ¿ëÇÑ ¹ßÇö¿¡ È°¿ëµÇ°í ÀÖ´Ù.
TransIT¢ç-Insect Transfection Reagent´Â ´Ù¾çÇÑ °ïÃæ¼¼Æ÷¿¡¼­ ´Ü¹éÁúÀ» °íÈ¿À²·Î ¹ßÇö½ÃÅ°±â À§ÇØ ÃÖÀûÈ­µÈ animal origin free transfection reagentÀÌ´Ù.
Data

±×¸²1. Trans IT¢ç-Insect Transfection Reagent¸¦ ÀÌ¿ëÇÑ È¿°úÀûÀÎ baculovirus DNA transfection
6 well plate¿¡ well ´ç 5 x 105 Sf9 cell¿¡ Trans IT¢ç-Insect reagent : DNA = 3:1 (¥ìl:¥ìg)) ºñÀ²·Î ÇÏ¿© GPF¸¦ ¹ßÇöÇÏ´Â ProGreen¢â baculovirus genomic vector DNA (AB Vector) 0.5 ¥ìg°ú pVL1393 transfer vector (AB Vector) 0.1 ¥ìgÀ» ÇÔ²² transfection ÇÏ¿´´Ù. 6ÀÏ ÈÄ Çü±¤ Çö¹Ì°æÀ¸·Î GFP ¹ßÇö Á¤µµ¸¦ È®ÀÎÇÏ¿´´Ù.


±×¸²2. Ÿ»ç ´ëºñ ³ôÀº virus »ý»êÈ¿À²
96 well plate¿¡ ¹è¾çµÈ Sf9, High Five¢â, Drosophila S2 ¼¼Æ÷¿¡ hr5 enhancer/IE1 promoter°¡ Æ÷ÇÔµÈ luciferase expression plasmid¸¦ 0.1§¶¾¿ reagent : DNA ratio (¥ìl: ¥ìg)¿¡ µû¶ó °¢°¢ transfection ÈÄ, 48½Ã°£ µÚ luciferase ¹ßÇöÀ» ÃøÁ¤ÇÏ¿´´Ù. Sf9, High Five¢â ¼¼Æ÷´Â serum-free media¿¡¼­ ¹è¾ç ÈÄ transfection ÇÏ¿´°í, Drosophila S2 ¼¼Æ÷´Â serumÀÌ Æ÷ÇÔµÈ ¹è¾ç Á¶°Ç¿¡¼­ ½ÇÇèÇÏ¿´´Ù.


±×¸²3. Ÿ»ç ´ëºñ ³ôÀº ÀçÁ¶ÇմܹéÁú ¹ßÇö È¿À² - High Five¢â ¼¼Æ÷ ÀÌ¿ë ¿¹
6 well plate¿¡ ¹è¾çÇÑ High Five¢â ¼¼Æ÷¿¡ hr5 enhancer/IE1 promoter°¡ Æ÷ÇÔµÈ GFP expression plasmid¸¦ 2.5§¶¾¿ reagent : DNA ratio (¥ìl: ¥ìg)¿¡ µû¶ó °¢°¢ transfection ÈÄ, 72½Ã°£ µÚ ¹è¾ç¾× 100 ¥ìlÀ¸·Î Á¶Á¦ÇÑ cell lysate¸¦ SDS-PAGE Àü±â¿µµ¿ ¹× Coomassie blue ¿°»öÇÏ¿© ´Ü¹éÁú ¹ßÇö ¿©ºÎ¸¦ È®ÀÎÇÏ¿´´Ù (transfection µÇÁö ¾ÊÀº ¼¼Æ÷ (cell alone)¸¦ ÄÁÆ®·Ñ·Î »ç¿ë).
6x His, S, HSV tagÀ» Æ÷ÇÔÇÏ´Â GFP (~38 kDa) ´Ü¹éÁúÀ» °¢ cell lysate¿¡¼­ È®ÀÎÇÒ ¼ö ÀÖ¾ú°í, ƯÈ÷ TransIT¢ç-Insect ·Î transfectionÇÑ »ùÇÿ¡¼­ Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº ´Ü¹éÁú ¹ßÇöÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.
º¸Á¸ : - 20¡É
°ü·ÃÁ¦Ç°
- All in One baculovirus system: BacPAK¢â Baculovirus Expression System
- ´Ü¹éÁú Á¾·ù¿¡ µû¶ó ¼±ÅÃÇÏ´Â baculovirus ½Ã½ºÅÛ: flashBAC¢â Baculovirus Expression Systems
- qPCR·Î ºü¸£°Ô ¿ª°¡ ÃøÁ¤: BacPAK¢â qPCR Titration Kit
- ´Ù¾çÇÑ transfer plasmid: pOET Transfer Plasmid & BacMam Transfer Plasmid
- ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculovirus: FoldHelper-ER3 ¿Ü

Keyword : v6104,v6100,v6103,v6105,v6106,mir6104,mir6100,mir6103,mir6105,mir6106