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Transfection ½Ã¾à
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¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯ÀüÀÚ µµÀÔ
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IT¢ç-Insect Transfection Reagent
°í¿ª°¡ÀÇ Baculvoirus »ý»êÀ» À§ÇÑ Àü¿ë½Ã¾à
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IT¢ç-Insect Transfection Reagent
Á¦Á¶»ç
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(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 6104
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IT-Insect Transfection Reagent
0.4 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 6100
Trans
IT-Insect Transfection Reagent
1 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 6105
Trans
IT-Insect Transfection Reagent
5 x 1 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 6106
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IT-Insect Transfection Reagent
10 x 1 ml
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Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
Cell line¿¡ ÀûÇÕÇÑ transfection ½Ã¾à ã±â
Sf9, High Five¢â, S2 µî ´Ù¾çÇÑ Insect cell lineÀÇ transfection¿¡ ÃÖÀû
³ôÀº È¿À²·Î Baculovirus »ý»ê °¡´É
Plaque assay¾øÀÌ ´Ü ±â°£¿¡ 1 step expression system ±¸Ãà ½ÇÇö
Serum compatibility - Media change°¡ ÇÊ¿ä ¾ø´Â Non-liposomal, animal-origin free formulation
Á¦Ç° ¼³¸í
°ïÃæ ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛÀº ¹ßÇöµÈ ´Ü¹éÁúÀÇ ¹ø¿ª ÈÄ °úÁ¤À» ±¸ÇöÇÒ ¼ö ÀÖ¾î À¯¿ëÇϸç, ÀϹÝÀûÀ¸·Î °ïÃæ¼¼Æ÷¿¡¼ÀÇ ÀϽÃÀûÀÎ ¹ßÇö ¶Ç´Â ÀçÁ¶ÇÕ baculovirus¸¦ ÀÌ¿ëÇÑ ¹ßÇö¿¡ È°¿ëµÇ°í ÀÖ´Ù.
Trans
IT
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-Insect Transfection Reagent´Â ´Ù¾çÇÑ °ïÃæ¼¼Æ÷¿¡¼ ´Ü¹éÁúÀ» °íÈ¿À²·Î ¹ßÇö½ÃÅ°±â À§ÇØ ÃÖÀûÈµÈ animal origin free transfection reagentÀÌ´Ù.
Data
±×¸²1.
Trans
IT
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-Insect Transfection Reagent¸¦ ÀÌ¿ëÇÑ È¿°úÀûÀÎ baculovirus DNA transfection
6 well plate¿¡ well ´ç 5 x 105 Sf9 cell¿¡
Trans
IT
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-Insect
reagent : DNA = 3:1 (¥ìl:¥ìg)) ºñÀ²·Î ÇÏ¿© GPF¸¦ ¹ßÇöÇÏ´Â ProGreen¢â baculovirus genomic vector DNA (AB Vector) 0.5 ¥ìg°ú pVL1393 transfer vector (AB Vector) 0.1 ¥ìgÀ» ÇÔ²² transfection ÇÏ¿´´Ù. 6ÀÏ ÈÄ Çü±¤ Çö¹Ì°æÀ¸·Î GFP ¹ßÇö Á¤µµ¸¦ È®ÀÎÇÏ¿´´Ù.
±×¸²2. Ÿ»ç ´ëºñ ³ôÀº virus »ý»êÈ¿À²
96 well plate¿¡ ¹è¾çµÈ Sf9, High Five¢â, Drosophila S2 ¼¼Æ÷¿¡ hr5 enhancer/IE1 promoter°¡ Æ÷ÇÔµÈ luciferase expression plasmid¸¦ 0.1§¶¾¿ reagent : DNA ratio (¥ìl: ¥ìg)¿¡ µû¶ó °¢°¢ transfection ÈÄ, 48½Ã°£ µÚ luciferase ¹ßÇöÀ» ÃøÁ¤ÇÏ¿´´Ù. Sf9, High Five¢â ¼¼Æ÷´Â serum-free media¿¡¼ ¹è¾ç ÈÄ transfection ÇÏ¿´°í, Drosophila S2 ¼¼Æ÷´Â serumÀÌ Æ÷ÇÔµÈ ¹è¾ç Á¶°Ç¿¡¼ ½ÇÇèÇÏ¿´´Ù.
±×¸²3. Ÿ»ç ´ëºñ ³ôÀº ÀçÁ¶ÇմܹéÁú ¹ßÇö È¿À² - High Five¢â ¼¼Æ÷ ÀÌ¿ë ¿¹
6 well plate¿¡ ¹è¾çÇÑ High Five¢â ¼¼Æ÷¿¡ hr5 enhancer/IE1 promoter°¡ Æ÷ÇÔµÈ GFP expression plasmid¸¦ 2.5§¶¾¿ reagent : DNA ratio (¥ìl: ¥ìg)¿¡ µû¶ó °¢°¢ transfection ÈÄ, 72½Ã°£ µÚ ¹è¾ç¾× 100 ¥ìlÀ¸·Î Á¶Á¦ÇÑ cell lysate¸¦ SDS-PAGE Àü±â¿µµ¿ ¹× Coomassie blue ¿°»öÇÏ¿© ´Ü¹éÁú ¹ßÇö ¿©ºÎ¸¦ È®ÀÎÇÏ¿´´Ù (transfection µÇÁö ¾ÊÀº ¼¼Æ÷ (cell alone)¸¦ ÄÁÆ®·Ñ·Î »ç¿ë).
6x His, S, HSV tagÀ» Æ÷ÇÔÇÏ´Â GFP (~38 kDa) ´Ü¹éÁúÀ» °¢ cell lysate¿¡¼ È®ÀÎÇÒ ¼ö ÀÖ¾ú°í, ƯÈ÷
Trans
IT
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-Insect ·Î transfectionÇÑ »ùÇÿ¡¼ Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº ´Ü¹éÁú ¹ßÇöÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.
º¸Á¸ : - 20¡É
°ü·ÃÁ¦Ç°
- All in One baculovirus system:
BacPAK¢â Baculovirus Expression System
- ´Ü¹éÁú Á¾·ù¿¡ µû¶ó ¼±ÅÃÇÏ´Â baculovirus ½Ã½ºÅÛ:
flash
BAC¢â Baculovirus Expression Systems
- qPCR·Î ºü¸£°Ô ¿ª°¡ ÃøÁ¤:
BacPAK¢â qPCR Titration Kit
- ´Ù¾çÇÑ transfer plasmid:
pOET Transfer Plasmid & BacMam Transfer Plasmid
- ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculovirus:
FoldHelper-ER3
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Keyword : v6104,v6100,v6103,v6105,v6106,mir6104,mir6100,mir6103,mir6105,mir6106
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯ÀüÀÚ µµÀÔ
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IT¢ç-293 Transfection Reagent
Trans
IT¢ç-BrCa Transfection Reagent
Trans
IT¢ç-CHO Transfection Kit
Trans
IT-HeLaMONSTER¢ç Transfection Kit
Trans
IT¢ç-Jurkat Transfection Reagent
Trans
IT¢ç-Keratinocyte Transfection Reagent
°í¿ª°¡ÀÇ Baculvoirus »ý»êÀ» À§ÇÑ Àü¿ë½Ã¾à
Trans
IT¢ç-Insect Transfection Reagent
Mouse ES ¼¼Æ÷¿¡ ÃÖÀûÈµÈ Transfection ½Ã¾à
Xfect mESC Transfection Reagent