Whole Genome Amplification (WGA)À» À§ÇÑ ¼¼°èÀûÀÎ Gold Standard ±â¼ú
PicoPLEX¢ç Á¦Ç°±º 20% ÇÒÀÎ ÇÁ·Î¸ð¼Ç ¾È³»
±â°£: 2024³â 5¿ù 2ÀÏ (¸ñ) ~ 6¿ù 28ÀÏ (±Ý)
³»¿ë: Whole Genome Amplification (WGA) kits – NGS library preparation kits ÀüÇ°¸ñ 20% ÇÒÀÎ
¡á PicoPLEX¢ç ±â¼úÀÇ ÀåÁ¡
¡¤ Single Cell ¶Ç´Â ±Ø¼Ò·®(6 pg ¼öÁØ) »ùÇÃÀ» ¸Å¿ì ³ôÀº °¨µµ¿Í ÀçÇö¼ºÀ¸·Î ÁõÆø ¹× ºÐ¼®
¡¤ À¯ÀüÀÚº¹Á¦º¯ÀÌ (CNV), ¿°»öü À̼ö¼º (Chromosomal aneuploidies), PGT-A/PGS/PGDÀÇ Gold standard
¡¤ °£´ÜÇÑ ½ÇÇè ¿öÅ©Ç÷οì: 1 tube¿¡¼ ½ÇÇè °úÁ¤ÀÌ ´Ü 3stepÀ¸·Î ½ÇÇè ÁøÇà
¡á PicoPLEX¢ç ±â¼úÀ̶õ?
¡¤ PicoPLEX¢ç´Â quasi-random primer¸¦ ÀÌ¿ëÇÑ linear whole genome pre-amplification °úÁ¤(step 2)°ú exponential amplification(step 3)À» ÀÌ¿ëÇÏ¿© ´ÜÀϼ¼Æ÷(single cell) ¶Ç´Â ÇÇÄڱ׶÷(pg) ¼öÁØÀÇ ±Ø¼Ò·® DNA¸¦ ÁõÆøÇÏ°í ÀçÇö¼ºÀÌ ³ôÀº NGS library¸¦ ±¸ÃàÇÏ´Â ±â¼úÀÌ´Ù.
(Step 1) ¼¼Æ÷ ¿ëÇØ(cell lysis)
(Step 2) Pre-amplification
- µ¶ÀÚÀûÀÎ quasi-random primer°¡ gDNA¿¡ ¼±ÅÃÀûÀ¸·Î °áÇÕ
- PCR ¿¡·¯¸¦ ÃÖ´ëÇÑ °¨¼Ò½ÃÅ°´Â quasi-linear amplification Àû¿ë
(Step 3) PCR Amplification
¡á PicoPLEX¢ç Á¦Ç° ¶óÀÎ
Code | Á¦Ç°¸í | ¸ñÀû | Àû¿ë |
R300718 | Single cells ȤÀº ¾à 15 pg DNA¸¦ ÁõÆø |
- Copy number variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR - Target enrichment or amplicon enrichment for mutation detection - SNP genotyping
| |
R300669 | Single cells ȤÀº ¾à 15 pg DNA¸¦ ÁõÆøÇÏ¿© Illumina¿ë library Á¦ÀÛ | - CNV, SNV, aneuploidy (¿°»öü À̼ö¼º) °ËÃâ |
*PicoPLEX¢ç Gold Single Cell DNA-seq Kit (Code R300669)¸¦ »ç¿ëÇϸé Çϱâ À̹ÌÁö¿Í °°ÀÌ hands-on time 1½Ã°£ À̳»·Î Illumina¢ç NGS library Á¦ÀÛÀ» ÇÒ ¼ö ÀÖ´Ù (Á¦À۽ð£: ~ 3½Ã°£).
¡á ±âŸ NGS Á¦Ç°±º
¡¤ Single cell RNA-Seq Àü¿ë Å°Æ®, SMART-Seq¢ç mRNA Single Cell (Code 634789)
¡¤ DNA¿¡¼ Illumina¿ë library¸¦ Á¦ÀÛÇÒ ¶§ »ç¿ëÇÏ´Â °í°¨µµ library prep. kit, ThruPLEX¢ç DNA-Seq Kit (Code R400674)
°ü·Ã ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ Áö¿ª ´ë¸®Á¡ (´ë¸®Á¡ ¾È³») ¶Ç´Â °í°´Áö¿ø (02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
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