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Human cDNA Library

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9503
cDNA Library, Human Brain
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¥ìg
897,600¿ø 
1,122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9503_DS.v1902Da.pdf
Takara
9504
cDNA Library, Human Heart
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¥ìg
897,600¿ø 
1,122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9504_DS.v1902Da.pdf
Takara
9505
cDNA Library, Human Liver
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¥ìg
897,600¿ø 
1,122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9505_DS.v1902Da.pdf
Takara
9506
cDNA Library, Human Lung
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¥ìg
897,600¿ø 
1,122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9506_DS.v1902Da.pdf
Takara
9511
cDNA Library, Human Placenta
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¥ìg
897,600¿ø 
1,122,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9511_DS.v1902Da.pdf

Á¦Ç°¼³¸í
º» Á¦Ç°Àº ´Ù¾çÇÑ polyA+ RNA¿¡¼­ adaptor ligation¹ý1) À¸·Î Á¦ÀÛÇÑ plasmid DNAÇü cDNA libraryÀÌ´Ù.
Á¦ÇÑÈ¿¼Ò Not I ÀνĺÎÀ§¸¦ °¡Áø oligo(dT)18 Linker primer¿Í BamH I (Bgl II)-Sma I adaptor¸¦ »ç¿ëÇÏ¿© unidirectional cloningÀ¸·Î library¸¦ Á¦ÀÛÇÏ¿´´Ù. Cloning Àü¿¡ ¾à 300 bp ÀÌÇÏÀÇ ´ÜÆíÀ» ¾î´À Á¤µµ Á¦°ÅµÇ¾ú°í, library´Â ÆòÆÇ ¹è¾ç¹ý¿¡ ÀÇÇØ 1¹ø¸¸ ÁõÆøµÈ ¼¼Æ÷¿¡¼­ plasmid ÃßÃâ kitÀ» »ç¿ëÇÏ°í Á¶Á¦µÇ¾ú´Ù.
1) Gubler and Hoffman procedure [Gene 25, 263-269 (1983)]
Á¦Ç°±¸¼º
¿ë·®: 5 §¶
Çü»ó: 10 mM Tris-HCl (pH8.0), 1 mM EDTA
³óµµ: 200 §¶/§¢
Cloning Vector
cDNA library¿¡ »ç¿ëÇÏ°í ÀÖ´Â pAP3neo vector´Â Okayama-Berg vector (pCD2)À» ¹ÙÅÁÀ¸·Î ¸¸µé¾îÁø °ÍÀ¸·Î SV40 ÇÁ·Î¸ðÅ͸¦ °¡Áö°í ÀÖ¾î Æ÷À¯·ù ¼¼Æ÷¿¡¼­ ¹ßÇö °¡´ÉÇÏ´Ù. ¶ÇÇÑ ssDNA»ý¼º¿¡ ÇÊ¿äÇÑ f1 ori, in vitro RNAÇÕ¼ºÀ» À§ÇÑ T7 ¹× T3 RNA ÁßÇÕ È¿¼Ò ÇÁ·Î¸ðÅ͸¦ °¡Áö°í ÀÖ´Ù(±×¸² 1).
GenBank Accession No.AB003468


±×¸² 1. pAP3neo vector map
Cloning site
1) Insert cDNA´Â pAP3neoÀÇ Bgl II */ Not I »çÀÌÆ®¿¡ cloning µÇ¾î ÀÖ´Ù.
*Adaptor BamH I (Bgl II)-Sma IÀ» ÀÌ¿ëÇÏ¿© cloning µÇ¾úÀ¸¹Ç·Î, cloning ÈÄ Bgl II site´Â ÀÌ¿ëÇÒ ¼ö ¾ø´Ù.
2) Xba I site´Â dam methylaseÀÇ ¿µÇâÀ» ¹Þ°í ÀÖ´Ù.
Human cDNA Library

Á¦Ç°¸í

Poly(A)+RNA source

Code

Primary
library size 1)
(Independent clone)

Ŭ·Î´× ÈÄ avr. 2)
insert size
(kbp)

Human Brain

male Caucasian age 57; cause of death, trauma, sudden death

9503

6.8¡¿106

1.0

Human Heart

pooled from male Caucasians, ages 13-73: cause of death, trauma

9504

6.7¡¿106

1.2

Human Liver

female Caucasian, age 58: cause of death, trauma

9505

2.1¡¿106

1.4

Human Lung

female Caucasian, age 42: cause of death, trauma, hemorrhage

9506

2.2¡¿106

1.2

Human Pancreas

pooled from male/ female Caucasians, ages 27-69: cause of death, trauma

9509

7.0¡¿106

1.1

Human Placenta

Caucasian, age 26

9511

6.6¡¿106

1.4


1) Primary library size (Independent clone): ÁõÆøÀü clone ¼ö
2) Ŭ·Î´× ÈÄ Æò±Õ insert size: ¹«ÀÛÀ§·Î ¼±º°ÇÑ 15°³ colonyÀÇ insert sizeÀÇ Æò±ÕÄ¡

Keyword :

cDNA Library (Human, Mouse, Rat)