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NDiff277

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Cellartis
Y40002
NDiff¢ç 227
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500 ml
°¡°Ý¹®ÀÇ   Y40002_DS.v2412.pdf

* [Àû¿ë] NDiff 277À» ÀÌ¿ëÇÑ mouse organoid Á¦ÀÛ

  • Á¢Âø ¹è¾ç»óÅ¿¡¼­ ¸¶¿ì½º ES ¼¼Æ÷ÀÇ ½Å°æ ºÐÈ­¿¡ »ç¿ë
  • ÀûÀýÇÑ ÀÎÀÚ¸¦ ÷°¡ÇÏ¿© ¸¶¿ì½º ES ¼¼Æ÷ ¹è¾çµµ °¡´É
Á¦Ç°¼³¸í
NDiff 227 media´Â Á¢Âø ´ÜÃþ ¹è¾ç Á¶°Ç(adherent monolayer culture condition)ÀÇ ¸¶¿ì½º ES ¼¼Æ÷¸¦ ½Å°æ ¼¼Æ÷·Î ºÐÈ­½ÃÅ°±â À§ÇÑ ¹«Ç÷û¹èÁö(serum-free media)ÀÌ´Ù(Á¶¼º ºñ°ø°³). ÀÌ media´Â ÷°¡ ÀÎÀÚ¸¦ Ãß°¡ÇÏ¿© ¸¶¿ì½º ES ¼¼Æ÷¸¦ feeder free culture¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù (Âü°í ¹®Çå 2). ¶ÇÇÑ ¼ºÀå ÀÎÀÚ¸¦ ÷°¡ÇÔÀ¸·Î½á Àΰ£ ES ¼¼Æ÷ ¹è¾ç¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù
(Âü°í ¹®Çå 3,4).
NDiff 227´Â FGF¸¦ ÷°¡ÇÏ¿© ¸¶¿ì½º ES ¼¼Æ÷¿¡¼­ °£À̳ª ÃéÀåÀ¸·Î ºÐÈ­ ÇÒ ¼ö ÀÖ´Â ADE (Anterior Definitive Endoderm) Àü±¸Ã¼¸¦ ³¾ ¼öµµ ÀÖ´Ù (Âü°í ¹®Çå 5).


Figure 1. Neural differentiation of mouse ES cells
º¸Á¸
-20 ¡É (À¶ÇØ ÈÄ 4 ¡É¿¡¼­ ÀúÀåÇÏ°í, 4 ÁÖ À̳»¿¡ »ç¿ë)
»ç¿ë ¿¹½Ã
[¸¶¿ì½º ES ¼¼Æ÷·ÎºÎÅÍ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­]
1. ³·Àº passageÀÇ feeder-free ES ¼¼Æ÷¸¦ 2.5 ~ 10 ¡¿ 10^3 cells / cm2 ¹Ðµµ·Î Á©¶óƾ ÄÚÆà Ç÷¹ÀÌÆ®¿¡ NDiff 227 ¹èÁö¸¦ ÀÌ¿ëÇÏ¿© ÆÄÁ¾ÇÑ´Ù.
2. 1 ~ 2ÀÏ ¸¶´Ù ¹èÁö¸¦ ±³È¯ÇÑ´Ù. ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­ Ãʱ⿡´Â »ó´ç¼öÀÇ Á×Àº ¼¼Æ÷°¡ °üÂûµÈ´Ù.
3. ¼¼Æ÷·ÎÀÇ ºÐÈ­¸¦ ÇüÅ °üÂû ¶ÇÇÑ ¸¶Ä¿ÀÇ ¹ßÇö µîÀ¸·Î °üÂûÇÑ´Ù. ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­´Â 4 ~ 6 ÀÏ°ºÎÅÍ °üÂûµÇ¾î 7 ~ 9 ÀÏ¿¡ ½Å°æ ¼º¼÷ÀÌ º¸ÀδÙ.
[ÁÖÀÇ] À§ Á¶°ÇÀº Ç¥ÁØÀÌ¸ç °¢ ¼¼Æ÷ÁÖ¿¡ ÀÇÇØ Á¶°Ç °ËÅä°¡ ÇÊ¿äÇÏ´Ù.

Âü°í ¹®Çå
1.   RYBP-PRC1 Complexes Mediate H2A Ubiquitylation at Polycomb Target Sites Independently of PRC2 and H3K27me3.
     Tavares L, et al. Cell 2012 148:664-678.
2.   Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
     Engelen E, et al. Nat Genet. 2011 43(6):607-11.
3.   Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays.
     Hook L, et al. Neurochem Int. 2011 59(3): 432-44.
4.   Anterior definitive endoderm from ESCs reveals a role for FGF signaling.
     Morrison G, et al. Cell Stem Cell 2008 3:402-415.
5.   Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
     Yao S, et al. PNAS 2006 103(18):6907-6912.
6.   Expansion of Human Embryonic Stem Cells in Defined Serum-Free Medium Devoid of Animal-Derived Products.
     Li Y, et al. Biotechnology and Bioengineering 2005 91:688-698
7.   Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
     Ying QL, et al. Nature Biotechnology 2003 21:183-186.
8.   BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3.
     Ying QL, et al. Cell 2003 115:281-292.
9.   Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors.
     Zhang K., et al. Protein Cell, 2014 DOI 10.1007/s13238-013-0011-2
10.  Imprinted expression of UBE3A in non-neuronal cells from a Prader-Willi syndrome patient with an atypical deletion.
      Martins-Taylor K., et al. Hum. Mol. Genet. 2013 doi: 10.1093/hmg/ddt628

Keyword :

Neural Stem cell (NSC)
>
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
>
Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2