Plasmid ÁغñºÎÅÍ in vitro transcription±îÁö Çѹø¿¡ Takara IVTpro¢â!!
´ç»ç¿¡¼´Â mRNA ÇÕ¼º ½ÇÇèÀ» À§ÇØ IVT¿ë template plasmid Á¦ÀÛ
Kit (Cloning Kit for mRNA
Template (Code 6143))¿Í template plasmid¿¡¼ ÃÖ°í ¼öÀ²·Î mRNA¸¦ ÇÕ¼ºÇÏ´Â IVT KitÀÎ (Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144))¸¦
Çϳª·Î ¹¾î Takara IVTpro¢â mRNA Synthesis System (Code 6141)·Î ÆǸÅÇÏ°í ÀÖ½À´Ï´Ù.
ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é ¾Æ·¡ÀÇ ±×¸²°ú °°ÀÌ KitÀÇ plasmid¿¡ In-Fusion¢ç ½Ã¾àÀ¸·Î CDS (Coding sequence)¸¦ cloningÀ» ÇÏ°í ÀÌÈÄ in vitro transcriptionÀ» ÁøÇàÇØ mRNA¸¦ ÇÕ¼ºÇÑ µÚ Á¤Á¦Çϱ⠱îÁöÀÇ
°úÁ¤À» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
±×¸² 1.
Takara IVTpro¢â ½Ã½ºÅÛ workflow
±×¸² 2.
Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)°ú ½ÃÁßÀÇ ´Ù¸¥ Á¦Ç°ÀÇ mRNA ÇÕ¼º ¼öÀ² ºñ±³
±×¸² 3.
Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)¸¦ »ç¿ëÇÑ ´Ù¾çÇÑ ±æÀÌÀÇ mRNA ÇÕ¼º °á°ú (CleanCap + m1¥× Á¶°Ç)
IVT °ü·ÃÁ¦Ç°
Code |
Á¦Ç°¸í |
¿ëµµ |
6143 |
¨ç Template plasmid Á¦ÀÛ | |
6144 |
Takara
IVTpro¢â T7 mRNA Synthesis Kit |
¨è ÃÖ°í ¼öÀ² mRNA ÇÕ¼º Kit |
6141 |
Takara
IVTpro¢â mRNA Synthesis System |
¨ç + ¨è |
2460A |
Capping enzyme (uncapped -> Cap0) | |
2470A |
mRNA
Cap 2-O-Methyltransferase |
Capping enzyme (Cap0 -> Cap1) |
1227A |
Template plasmid ¼±ÇüÈ | |
6146 |
Template
Vector (BspQ I) for T7 mRNA Synthesis |
BspQ I¿ë IVT plasmid |