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NEWS
  : [] mRNA ռ Ÿ!! Takara IVTpro ø
ۼ : 2023.12.26
 ۼ : īڸ


 

Plasmid غ in vitro transcription ѹ Takara IVTpro!!


mRNA ռ IVT template plasmid Kit (Cloning Kit for mRNA Template (Code 6143)) template plasmid ְ mRNA ռϴ IVT Kit (Takara IVTpro T7 mRNA Synthesis Kit (Code 6144)) ϳ  Takara IVTpro mRNA Synthesis System (Code 6141) Ǹϰ ֽϴ.

ǰ ϸ Ʒ ׸ Kit plasmid In-Fusion þ CDS (Coding sequence) cloning ϰ in vitro transcription mRNA ռ ϱ ֽϴ.

 


׸ 1. Takara IVTpro ý workflow 

 


׸ 2. Takara IVTpro T7 mRNA Synthesis Kit (Code 6144) ٸ ǰ mRNA ռ  



 
׸ 3. Takara IVTpro T7 mRNA Synthesis Kit (Code 6144) پ mRNA ռ (CleanCap + m1 )

 

IVT ǰ

Code

ǰ

6143

Cloning Kit for mRNA Template

Template plasmid

6144

Takara IVTpro T7 mRNA Synthesis Kit

ְ mRNA ռ Kit

6141

Takara IVTpro mRNA Synthesis System

+

2460A

Vaccinia Capping Enzyme

Capping enzyme (uncapped -> Cap0)

2470A

mRNA Cap 2-O-Methyltransferase

Capping enzyme (Cap0 -> Cap1)

1227A

BspQ I

Template plasmid ȭ

6146

Template Vector (BspQ I) for T7 mRNA Synthesis

BspQ I IVT plasmid