Á¦¸ñ : [ÇÁ·Î¸ð¼Ç] ´Ù¾çÇÑ DNA »ùÇÃÀÇ NGS, PFA·Î °íÁ¤ÇÑ single cellÀÇ WGA ºÐ¼®À» ¿øÇÑ´Ù¸é? ´ÙÄ«¶ó NGS Kit Àüü 20% ÇÒÀÎ Áß (~6/19)
´Ù¾çÇÑ DNA »ùÇÿ¡¼ °íǰÁúÀÇ NGS library Á¦ÀÛÀ» À§ÇÑ
´ÙÄ«¶ó NGS Kit! 20% ÇÒÀÎ Áß
FFPE, cell-free DNA NGS libraryÁ¦ÀÛ¿¡ ÃÖÀû
- ±Ø¼Ò·® (pg¼öÁØ) fragmented dsDNA¸¸ ÀÖÀ¸¸é Illumina ºÐ¼®¿ë NGS library Á¦ÀÛ °¡´É
- ºÐ¼®ÀÌ ¾î·Á¿î FFPE À¯·¡ DNA, cfDNA µîÀÇ ºÐ¼®¿¡ ÃÖÀû
- Input¾ç¿¡ °ü°è¾øÀÌ ÀçÇö¼ºÀÌ ³ô°í ±ÕÀÏÇÑ Ä¿¹ö¸®Áö ½ÇÇö, GC ÇÔ·®ÀÌ ³ôÀº ¿µ¿ªµµ ±ÕÀÏÇÑ Ä¿¹ö¸®Áö À¯Áö
- °£ÆíÇÑ single tube workflow·Î »ùÇà loss ¹æÁö ¹× ½Ã°£ Àý¾à °¡´É
±×¸² 1. ThruPLEX¢ç DNA-Seq FLEX¸¦ ÀÌ¿ëÇÑ single tube, 3-step workflow
PicoPLEX¢ç ±â¼ú·Î ³ôÀº ÀçÇö¼ºÀÇ WGA ºÐ¼®
- PFA °íÁ¤ single celló·³ ºÐ¼®ÀÌ ¾î·Á¿î ±Ø¼Ò·® »ùÇõµ °¡´É
- Illumina¿ë NGS library Á¦ÀÛÀ» 3½Ã°£¸¸¿¡ Á¦ÀÛ °¡´É
±×¸² 2. Quasi-random primer¸¦ ÀÌ¿ëÇÏ¿© ¼Ò·®ÀÇ »ùÇ÷κÎÅÍ ´Ù·®ÀÇ library¸¦ Á¦ÀÛ
NGS library Á¦ÀÛ Àü, pre-amplification ¹× Ÿ°Ù È®ÀÎÀ» À§ÇÑ High Fidelity PCR È¿¼Ò!
PrimeSTAR¢ç ½Ã¸®Áî
PrimeSTAR¢ç Max Ver. 2 | PrimeSTAR¢ç GXL | PrimeSTAR¢ç LongSeq |
R047A | R050A | R055A |
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°¡Àå Á¤È®ÇÑ PCR È¿¼Ò (Wild-type Taqº¸´Ù 420X ³ôÀº Á¤È®µµ) ½ÅÀå½Ã°£ 5~10ÃÊ·Î °í¼Ó¹ÝÀÀ Cloning¿ë insert PCR¿¡ ÃÖÀû | Long rangeÀÇ high fidelity È¿¼Ò ~30 kb & GC rich target¿¡ ÃÖÀû È¿¼Ò 2¹è »ç¿ë ½Ã 10 sec/kbÀÇ °í¼Ó¹ÝÀÀ | Super long target¿ë high fidelity È¿¼Ò ~50 kb & GC/AT rich target¿¡ ÃÖÀû Long read NGS, multiplex µî ´Ù¾çÇÑ application Àû¿ë °¡´É |
´ÙÄ«¶ó "´ë¿ë·® ÇÒÀο¡ ºüÁö´Ù: Cloning & Virus" ÇÁ·Î¸ð¼Ç Áß (~6/19) (ÀÚ¼¼ÇÑ ³»¿ëÀº ¾Æ·¡ ¹è³Ê¸¦ Ŭ¸¯ÇØÁÖ¼¼¿ä)
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