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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Hepatocyte > Hepatocyte from human iPSC

Hepatocyte from human iPSC

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Cellartis
Y10133
Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC12) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
°¡°Ý¹®ÀÇ   x98236
Cellartis
Y10134
Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC18) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
°¡°Ý¹®ÀÇ   x98236
Cellartis
Y10135
Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC22) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
°¡°Ý¹®ÀÇ   x98236


Á¦Ç°Æ¯Â¡
  • 3ÁÖ°£ Àå±â¹è¾ç°¡´É
  • ´Ù¾çÇÑ CYPȰ¼º À¯Áö
  • °¢Á¾ Hepatocyte metabolism È®ÀÎ
  • Lot-to-lot variation ÃÖ¼ÒÈ­
  • ¹è¾ç¿¡ ÇÊ¿äÇÑ ±¸¼ºÇ°À» ¸ðµÎ Æ÷ÇÔÇÑ All-in-one Á¦Ç°

Cellartis¢ç Enhanced hiPS-HEP (Y10050, Y10056, Y10058) Á¦Ç°ÀÌ °³¼±µÇ¾î Cellartis¢ç Enhanced hiPS-HEP v2(Y10133, Y10134, Y10135)·Î ¹öÀü¾÷!


±×¸² 1. ±âÁ¸ÀÇ Cellartis¢ç Enhanced hiPS-HEP (HEP v1) Á¦Ç°°ú Cellartis¢ç Enhanced hiPS-HEP v2 (HEP v2)Á¦Ç°ÀÇ ¹è¾ç±â°£°ú Assay ±â°£ ºñ±³
Á¦Ç°¼³¸í
Cellartis¢ç Enhanced hiPS-HEP v2´Â ±âÁ¸ Cellartis¢ç Enhanced hiPS-HEPÁ¦Ç°°ú ºñ±³ÇÏ¿© 20Àϰ£ÀÇ Àå±â¹è¾ç´É·Â°ú ´Ù¾çÇÑ hepatocyte¼¼Æ÷ ±â´ÉÀ» Çâ»ó½ÃŲ Á¦Ç°ÀÌ´Ù.
Y10133, Y10134, Y10135 Á¦Ç°Àº °¢±â ´Ù¸¥ ¼¼Æ÷ÁÖ¿¡¼­ À¯·¡µÈ Á¦Ç°À¸·Î ¼­·Î ´Ù¸¥ genetic background¸¦ °¡Áø´Ù. <¼¼Æ÷ÁÖ Á¤º¸ ¹Ù·Î°¡±â>

º» Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖ´Â ¼¼Æ÷´Â CYP (CYP1A, CYP2C9, CYP3A,µî) Ȱ¼ºÀÌ 20ÀÏ µ¿¾È ¾ÈÁ¤ÇϹǷÎ, in vitro¿¡¼­ µ¶¼º ´ë»ç, °£µ¶¼º, metabolism assay¿¡ ÀûÇÕÇÏ´Ù. ¶ÇÇÑ ¹ÙÀÌ·¯½º °¨¿°°ú ¹é½Å °³¹ß ¿¬±¸µîÀÇ Æò°¡¿¡ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, Á¦Ç°ÀÇ lot°£ ÆíÂ÷°¡ Àû¾î ÀÏÁ¤Çϰí ÀçÇö¼º ÀÖ´Â °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.

Human iPS ¼¼Æ÷ À¯·¡ °£¼¼Æ÷(hepatocyte)´Â human primary hepatocyte¿¡¼­ º¸ÀÌ´Â °¢Á¾ metabolismȰµ¿À» º¸ÀÌ¸ç °³¹ß°úÁ¤¿¡¼­ ¼¼Æ÷ÀÇ LDL, glycogenÀúÀå´É·Â, urea »ý¼º´É·Â ¹× albumin ¹× AFP ¹ßÇöÀ» °ËÁõÇÏ¿´´Ù.

Cellartis¢ç Enhanced hiPS-HEP v2 kit¿¡´Â ¼¼Æ÷ ȸº¹°ú ¹è¾ç¿¡ ÇÊ¿äÇÑ supplement kit¹× hepatocyte Àå±â¹è¾çÀ» À§ÇÑ ¹èÁö°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç ±âº»ÀûÀÎ ¼Ò¸ðǰ ¿Ü¿¡ ¹è¾çÀ» À§ÇÑ
Ãß°¡ÀûÀÎ Á¦Ç°±¸ÀÔÀÌ ÇÊ¿ä ¾ø´Â all-in-one kitÀÌ´Ù.
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  • Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC12) Kit (Code Y10133)
       - Cellartis¢ç Enhanced hiPS-HEP (from ChipSC12) (1 vial - 12 ¡¿ 106 viable cells)
         (¡Ø One 96 well plate ¶Ç´Â multi-well plate Àû¿ë °¡´É)
       - Cellartis¢ç Enhanced hiPS-HEP Thawing and Plating Kit
       - Cellartis¢ç Enhanced hiPS-HEP Long-Term Maintenance Medium
  • Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC18) Kit (Code Y10134)
       - Cellartis¢ç Enhanced hiPS-HEP (from ChipSC18) (1 vial - 12 ¡¿ 106 viable cells)
       - Cellartis¢ç Enhanced hiPS-HEP Thawing and Plating Kit
       - Cellartis¢ç Enhanced hiPS-HEP Long-Term Maintenance Medium
  • Cellartis¢ç Enhanced hiPS-HEP v2 (from ChiPSC22) Kit (Code Y10135)
       - Cellartis¢ç Enhanced hiPS-HEP (from ChipSC22) (1 vial - 12 ¡¿ 106 viable cells)
       - Cellartis¢ç Enhanced hiPS-HEP Thawing and Plating Kit
       - Cellartis¢ç Enhanced hiPS-HEP Long-Term Maintenance Medium
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- 80 ¡É
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  • Cellartis¢ç Enhanced hiPS-HEP : ¾×üÁú¼Ò(Liquid nitrogen)
    - ¼¼Æ÷´Â ¼ö·É ÈÄ Áï½Ã ¾×üÁú¼Ò¿¡ º¸°üÇϽʽÿÀ. ¾×üÁú¼Ò ÀúÀåÀÌ ºÒ°¡´ÉÇÑ °æ¿ì, Áï½Ã ¼¼Æ÷¸¦ ³ì¿© ¹è¾çÇϼ¼¿ä.
  • Cellartis¢ç Enhanced hiPS-HEP Thawing and Plating Kit (Y10132) : -20 ¡É
  • Cellartis¢ç Enhanced hiPS-HEP Long-Term Maintenance Medium (Y30052) : -20 ¡É


±×¸² 2. Enhanced hiPS-HEP v2¿Í primary °£¼¼Æ÷ CYPÈ¿¼Ò mRNA ¹ßÇö ºñ±³
¹è¾ç 24½Ã°£ ÈÄ human primary °£¼¼Æ÷¿Í(»ó´ë°ª 1) ¹è¾ç 20ÀÏ ÈÄEnhanced hiPS-HEP v2ÀÇ CYP mRNA¹ßÇöÀ» qPCR·Î ºñ±³Çß´Ù. ±× °á°ú CYP3A4, 3A5, 3A7, 1A2, 2C19ÀÇ ¹ßÇöÀº Cellartis Enhanced hiPS-HEP v2¿¡¼­primary°£¼¼Æ÷¿Í À¯»çÇÔÀ» È®ÀÎÇß´Ù.


±×¸² 3. ¾ËºÎ¹Î »ý¼º´É·Â
a. hiPS-HEP v2(C18) ¹è¾ç 12 Àϰ, Àΰ£ °£¼¼Æ÷´Â ¹è¾ç 24 ½Ã°£ ÈÄ¿¡ ¾ËºÎ¹ÎÀÇ ¹ßÇöÀ» È®ÀÎÇß´Ù.
b. hiPS-HEP v2(n=2) ¹è¾ç 13 Àϰ¿Í 20 Àϰ¿¡ Àΰ£ °£¼¼Æ÷(n =3)´Â ¹è¾ç 24 ½Ã°£ ÈÄ¿¡ ¾ËºÎ¹Î ¾çÀ» ELISA·Î ÃøÁ¤Çß´Ù. ±× °á°ú Àΰ£ °£¼¼Æ÷¿Í µ¿µîÇÑ ¾çÀ» »ý¼ºÇÔÀ» È®ÀÎÇÒ ¼ö ÀÖ´Ù.


hiPS-HEP v2 (C18)

Àΰ£ °£¼¼Æ÷

±×¸²4. Glycogen ÀúÀå´É·Â
hiPS-HEP´Â ¹è¾ç ÈÄ 12 Àϰ¿¡, Àΰ£ °£¼¼Æ÷´Â 24 ½Ã°£ ÈÄ PAS ¿°»öÀ» ½Ç½ÃÇß´Ù. ¼¼Æ÷ ³» ±Û¸®ÄÚ°ÕÀÌ ÀúÀåµÇ´Â °Í (ÇÎÅ©)À» È®ÀÎÇß´Ù.


±×¸²5. Cellartis Enhanced hiPS-HEP v2¸¦ ÀÌ¿ëÇÑ ¸¸¼º µ¶¼º ½ÃÇè
À¶ÇØÇÑ hiPS-HEP¿¡ Amiodarone, Aflatoxin, Troglitazone, ChlorpromazineÀ» ó¸® ÈÄ (¹è¾ç 4ÀÏ¿¡¼­ 18ÀÏ »çÀÌ) ¹è¾ç 2 Àϰ, 7 Àϰ, 14 Àϰ¿¡ ¼¼Æ÷ Áõ½Ä ºÐ¼®À» ½Ç½ÃÇß´Ù. 
±× °á°ú (a) Àΰ£ °£¼¼Æ÷¿Í µ¿ÀÏÇÑ º¯È­¸¦ º¸¿´À¸¸ç (b) ³óµµ ÀÇÁ¸ÀûÀÎ °¨¼ö¼ºÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù. ÀÌ´Â ÀÌ ¼¼Æ÷°¡ Àå±â ¸¸¼º µ¶¼º ½ÃÇè¿¡ »ç¿ëµÉ ¼ö ÀÖÀ½À» º¸¿©ÁØ´Ù.

1) À¶ÇØÁ÷ÈÄ


3) 12ÀÏ ÈÄ
2) 4ÀÏ ÈÄ


4) 19ÀÏ ÈÄ
±×¸²6. Enhanced hiPS-HEP v2ÀÇ À¶ÇØ ÈÄ 19ÀϱîÁö ¼¼Æ÷ ÇüŸ¦ È®ÀÎÇß´Ù.
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1. Annika Asplund et al . 
One Standardized Differentiation Procedure Robustly Generates HomogenousHepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells . 
Stem Cell Rev. 2016 12 (1) : 90-104.
2. Nidal, G. et al . 
Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines 
Stem Cells International . 2016 10 .1155 / 2016 / 8648356.
3. Ulvestad M et al.
Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells . 
Biochem Pharmacol. 2013 Sep 1; 86 (5) : 691-702.
4. Yildirimman R et al.
Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. 
Toxicol. Sci. 2011 Dec; 124 (2) : 278-290.
5. Mantel N et al.
Potential markers of attenuation of YF virus after infection of stem cell -derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus. 
6. Heins et al. Stem Cells 2004; 22 : 367-376. 
United States National Stem Cell Bank; http://www.nationalstemcellbank.org
°ü·ÃÁ¦Ç°
- Human albumin ÃøÁ¤¿ë pre-coated EIA kit: Human Albumin EIA Kit
- Mouse albumin ÃøÁ¤¿ë pre-coated EIA kit: Mouse Albumin EIA Kit

Keyword : °£¼¼Æ÷,HepaRG,HepG2,toxicitytest,µ¶¼º½ÃÇè,°£µ¶¼º