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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Cardiomyocyte > ÃÊ°í¼øµµ Cardiomyocyte from human iPSC
hiPS cell À¯·¡ÀÇ ½É±Ù¼¼Æ÷

ÃÊ°í¼øµµ Cardiomyocyte from human iPSC

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Cellartis
Y50025
MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
°¡°Ý¹®ÀÇ   ÁÖ¹® Á¦ÀÛ Y50025_e.v2008.pdf
Cellartis
Y50023
MiraCell¢ç CM Culture Medium v2
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
°¡°Ý¹®ÀÇ  
Cellartis
Y50015
MiraCell¢ç Cardiomyocytes (from ChiPSC12) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 Kit
°¡°Ý¹®ÀÇ   ÁÖ¹® Á¦ÀÛ Y50015_e.v2008.pdf
Cellartis
Y50013
MiraCell¢ç CM Culture Medium
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
°¡°Ý¹®ÀÇ  


* MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12) Kit (Code Y50025)¿Í MiraCell¢ç Cardiomyocytes (from ChiPSC12) Kit (Code Y50015)´Â ÁÖ¹® ÈÄ Á¦À۵Ǵ Á¦Ç°ÀÌ´Ï, ÀÏÁ¤¿¡ Âü°í ¹Ù¶ø´Ï´Ù.
* MED probe¸¦ ÀÌ¿ëÇÑ ½ÉÀå µ¶¼º Æò°¡ ÇÁ·ÎÅäÄÝ (1) ÇØµ¿ ÈÄ MED probe·Î ¼¼Æ÷¸¦ ¿Å±æ ¶§
* MED probe¸¦ ÀÌ¿ëÇÑ ½ÉÀå µ¶¼º Æò°¡ ÇÁ·ÎÅäÄÝ (2) MED probe ³» ¹Ù·Î ÇØµ¿ÇÒ ¶§
Á¦Ç°¼³¸í
  • ½É±Ù ¼¼Æ÷ÀÇ ¼øµµ 95%ÀÌ»ó (cTnT¾ç¼º·ü)
  • Ç×»ýÁ¦¸¦ »ç¿ëÇÑ Á¤Á¦ °úÁ¤ ¾øÀÌ °í¼øµµ ºÐÈ­
  • ÀÚÀ² ¹Úµ¿ ´É·Â º¸À¯
  • °¢Á¾ À̿ Åë·Î À¯ÀüÀÚ¸¦ ¹ßÇö (SCN5A, KCNQ1, CACNA1C, KCNH2µî)
  • °¢Á¾ À̿ Åë·Î Â÷´ÜÁ¦(E-4031, Chromanol 293B, Verapamil, Mexiletine)¿¡ ´ëÇÑ Àü±â »ý¸®ÇÐÀû ¹ÝÀÀ¼º

MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12)´Â Àΰ£ iPS¼¼Æ÷¿¡¼­ ºÐÈ­µÈ °í¼øµµ ½É±Ù¼¼Æ÷ÀÌ¸ç ½É±Ù ¼¼Æ÷ÀÇ Æ¯Â¡°ú ±â´É ÇØ¼® µî¿¡ »ç¿ë °¡´ÉÇÏ´Ù. ¶ÇÇÑ ½É±Ù¼¼Æ÷ÀÇ »ý¸®ÇÐ ÀûÀÎ ±â´ÉÀÇ ÇØ¼®°ú MEA (Multi-electrode array) ½Ã½ºÅÛ µîÀ» ÀÌ¿ëÇÑ ´Ù¾çÇÑ ¾àÁ¦¿¡ ÀÇÇÑ ½ÉÀå µ¶¼º ½ÃÇè µî¿¡ ´ëÇØ¼­µµ Æø³Ð°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº ±³Åä ´ëÇÐ iPS¼¼Æ÷ ¿¬±¸¼Ò¿¡¼­ °³¹ßµÈ Àΰ£ ½É±Ù ¼¼Æ÷ÀÇ Á¦ÀÛ ±â¼úÀ» Takara Bio°¡ iHeart Japan¢ßÀ¸·ÎºÎÅÍ µµÀÔÇÏ¿© °øµ¿ °³¹ßÇÑ Á¦Ç°ÀÌ´Ù. º» ½É±Ù¼¼Æ÷ Á¦ÀÛ ±â¼úÀº ½É±Ù ƯÀÌÀû ÇÁ·Î¸ðÅÍ (¥á-MHCµî)¸¦ ÀÌ¿ëÇÑ Ç×»ýÁ¦(¿¹: puromycine µî) ¼±º° °úÁ¤ ¾øÀÌ °í¼øµµ·Î ½É±Ù ¼¼Æ÷·Î ºÐÈ­°¡ °¡´ÉÇϰí, Àå±â°£ÀÇ ¹è¾ç ½Ã ÀϹÝÀûÀ¸·Î ¹ß»ýÇÏ´Â ¼øµµÀÇ ÀúÇϰ¡ 90Àϰ£ ¹è¾ç ÈÄ¿¡µµ °üÂûµÇÁö ¾Ê¾Ò´Ù (³í¹® Åõ°í Áß).
º» Á¦Ç°ÀÇ Á¦Á¶¿¡ »ç¿ëµÈ human iPS cell line ChiPSC12 (Code Y00285)Àº feeder-free ¹è¾ç Á¶°ÇÀÎ Cellartis¢ç DEF-CS¢â 500 Culture System (Code Y30010)À¸·Î ¹è¾çµÇ¾ú´Ù.

º» Á¦Ç°¿¡´Â ¼¼Æ÷¿Í Àΰ£ ES/iPS¼¼Æ÷ À¯·¡ ½É±Ù ¼¼Æ÷ À¶ÇØ¿ë ¹èÁö (MiraCell¢ç CM Thawing Medium), ½É±Ù ¼¼Æ÷ ¹è¾ç¿ë ¹èÁö (MiraCell¢ç CM Culture Medium v2)°¡ Æ÷ÇԵǾîÀÖ´Ù.
¡Ø MiraCell¢ç Cardiomyocyte v2 (from ChiPSC12) Kit (Code Y50025)´Â ±âÁ¸ Á¦Ç° (MiraCell¢ç Cardiomyocyte (from ChiPSC12) Kit (Code Y50015)ÀÇ ±âº»ÀûÀΠƯ¡ (¼øµµ, viability, ºÎÂøÈ¿À², E-4031 ¹ÝÀÀ¼º µî)À» À¯ÁöÇϰí ÀÖ¾î, ½ÉÀåµ¶¼º Æò°¡¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, MiraCell¢ç Cardiomyocyte v2 (from ChiPSC12) Kit´Â FPD (Filed potential duration, by Fridericia) ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï ½ÉÀå ¹Úµ¿¼ö°¡ Áõ°¡µÇ¾ú´Ù.
³»¿ë

MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12) Kit (Code Y50025)

MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12)

1 vial

£¾3 x 106 cells

MiraCell¢ç CM Thawing Medium

1 bottle

20 ml

MiraCell¢ç CM Culture Medium v2 (Code Y50023)

1 bottle

100 ml


MiraCell¢ç Cardiomyocytes (from ChiPSC12) Kit (Code Y50015)

MiraCell¢ç Cardiomyocytes (from ChiPSC12)

1 tube

£¾3 x 106 cells

MiraCell¢ç CM Thawing Medium

1 bottle

20 ml

MiraCell¢ç CM Culture Medium (Code Y50013)

1 bottle

100 ml


¼ö¼Û

MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12) Kit (Code Y50025)

MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12)

-80 ¡É

MiraCell¢ç CM Thawing Medium, MiraCell¢ç CM Culture Medium v2

-20 ¡É


MiraCell¢ç Cardiomyocytes (from ChiPSC12) Kit (Code Y50015)

MiraCell¢ç Cardiomyocytes (from ChiPSC12)

-80 ¡É

MiraCell¢ç CM Thawing Medium, MiraCell¢ç CM Culture Medium

-20 ¡É

º¸°ü
MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12) Kit (Code Y50025)
MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12): ¾×ü Áú¼Ò º¸Á¸
¼ö·É ÈÄ, Áï½Ã ¾×ü Áú¼Ò¿¡ º¸°üÇϼ¼¿ä.
¾×ü Áú¼Ò º¸Á¸ÀÌ ºÒ°¡´ÉÇϸé, ¹Ù·Î ¼¼Æ÷¸¦ À¶ÇØÇÏ¿© ¹è¾çÇϼ¼¿ä.

MiraCell¢ç CM Thawing Medium, MiraCell¢ç CM Culture Medium v2-20¡É ÀÌÇÏ, À¶ÇØ ÈÄ 4¡É
-20¡É ÀÌÇÏ¿¡¼­ µ¿°á º¸Á¸Çϰí, »ç¿ë Àü¿¡ 4¡É¿¡¼­ overnightÀ¸·Î ¹èÁö¸¦ ÇØµ¿À» Çϼ¼¿ä
À¶ÇØ ÈÄ¿¡´Â 4¡É¿¡¼­ º¸Á¸Çϰí, MiraCell¢ç CM Thawing MediumÀº 1ÁÖÀÏ À̳»,
MiraCell¢ç CM Culture Medium v2´Â 1°³¿ù À̳» »ç¿ëÇϼ¼¿ä(´Ù½Ã µ¿°á À¶ÇØ´Â ºÒ°¡).

MiraCell¢ç Cardiomyocytes (from ChiPSC12) Kit (Code Y50015)
MiraCell¢ç Cardiomyocytes (from ChiPSC12): ¾×ü Áú¼Ò º¸Á¸
¼ö·É ÈÄ, Áï½Ã ¾×ü Áú¼Ò¿¡ º¸°üÇϼ¼¿ä.
¾×ü Áú¼Ò º¸Á¸ÀÌ ºÒ°¡´ÉÇϸé, ¹Ù·Î ¼¼Æ÷¸¦ À¶ÇØÇÏ¿© ¹è¾çÇϼ¼¿ä.

MiraCell¢ç CM Thawing Medium, MiraCell¢ç CM Culture Medium: -20¡É ÀÌÇÏ, À¶ÇØ ÈÄ 4¡É
-20¡É ÀÌÇÏ¿¡¼­ µ¿°á º¸Á¸Çϰí, »ç¿ë Àü¿¡ 4¡É¿¡¼­ overnightÀ¸·Î ¹èÁö¸¦ ÇØµ¿À» Çϼ¼¿ä
À¶ÇØ ÈÄ¿¡´Â 4¡É¿¡¼­ º¸Á¸Çϰí, MiraCell¢ç CM Thawing MediumÀº 1ÁÖÀÏ À̳»,
MiraCell¢ç CM Culture Medium´Â 1°³¿ù À̳» »ç¿ëÇϼ¼¿ä(´Ù½Ã µ¿°á À¶ÇØ´Â ºÒ°¡).
»ç¿ë »óÀÇ ÁÖÀÇ
MiraCell¢ç Cardiomyocytes v2 (from ChiPSC12)Kit, MiraCell¢ç Cardiomyocytes (from ChiPSC12)Kit´Â iHeart Japan¢ß·ÎºÎÅÍ ¶óÀ̼±½º¸¦ ¹Þ°í Takara Bio°¡ Á¦Á¶, ÆÇ¸ÅÇϰí ÀÖ½À´Ï´Ù.


±×¸² 1. MiraCell¢ç Cardiomyocytes(from ChiPSC12)ÀÇ lot°£ Â÷ÀÌ(3 lot Á¦Ç°)ÀÇ È®ÀÎ
A) ½É±Ù ¸¶Ä¿ cTnT(Cardiac TroponinT)¾ç¼º·ü(ÇØµ¿ 2Àϰ)ºñ±³ °á°ú
B) ÇØµ¿ Á÷ÈÄ ¹× ÇØµ¿ 2Àϰ »ýÁ¸¼¼Æ÷ ¼ö ºñ±³ °á°ú
C) MED Probes(Alpha MED Scientific Inc.)¿¡¼­ FPDcF(Field potential duration corrected by Fridericia's formula)ÀÇ ºñ±³ °á°ú ¡ØBPM35ÀÌ»óÀÇ Probe¸¦ »ç¿ë
D) °¢ lotÀÇ IKr ÀúÇØÁ¦(E-4031)¿¡ ÀÇÇÑ FPDc10(FPDcF°¡ 10% ´õ ¿¬ÀåÇÒ ¶§ ¾àÁ¦ ³óµµ)ºñ±³ °á°ú
¾î´À Æò°¡ ¹æ¹ý¿¡¼­µµ lot °£ Â÷ÀÌ´Â ÀûÀº °ÍÀ¸·Î È®ÀεƴÙ.


±×¸² 2. MiraCell¢ç Cardiomyocytes(from ChiPSC12)À» ÀÌ¿ëÇÑ ¾àÁ¦ ¹ÝÀÀ¼º ½ÃÇè
ÇØµ¿ÇÑ ÈÄÀÇ ½É±Ù¼¼Æ÷¸¦ MED Probes(Alpha MED Scientific Inc.)¿¡ ¹è¾ç 4~9Àϰ¿¡ BPM (Beats per minute)ÀÌ 35 ÀÌ»óÀ» ³ªÅ¸³»´Â Probe¿¡ ´ëÇØ, ¼¼Æ÷ ¿Ü ÀüÀ§ ÃøÁ¤ÀåÄ¡¸¦ ÀÌ¿ëÇÏ¿© ¾à¹°¿¡ ´ëÇÑ ¹ÝÀÀ¼ºÀ» È®ÀÎÇß´Ù. IKrÀúÇØÁ¦ (E-4031), IKs¾ïÁ¦Á¦ (Chromanol 293B), Naä³Î ¹× IKrÀúÇØÁ¦ (Mexiletine)¿¡ ´ëÇØ¼­´Â FPDcFÀÇ ¿¬Àå°ú Caä³Î ÀúÇØÁ¦ÀÎ Verapamil¿¡ ´ëÇØ¼­´Â FPDcFÀÇ ´ÜÃàÀÌ È®ÀεƴÙ. ¶ÇÇÑ E-4031 ¹× Mexiletine¿¡ ´ëÇØ °í³óµµ½Ã ºÎÁ¤¸Æ ÇüÅÂÀÇ ÆÄÇü (EAD/TA; Early after depolarization/Triggered activity)ÀÌ °üÂûµÆ´Ù.


±×¸² 3. MiraCell¢ç Cardiomyocytes(from ChiPSC12) ½É±Ù °áÇÕ ´Ü¹éÁúÀÇ ¹ßÇö ÇØ¼®
FibronectinÀ¸·Î ÄÚÆÃµÈ ¹è¾ç Ç÷¹ÀÌÆ®·Î °è´ëÇÑ ÇØµ¿ ÈÄÀÇ ½É±Ù ¼¼Æ÷¸¦ 4% paraformaldehyde·Î °íÁ¤ÇÏ°í °¢ ´Ü¹éÁú¿¡ ƯÀÌÀûÀÎ Ç×ü¸¦ ÀÌ¿ëÇÏ¿© ¿°»öÇß´Ù. ±× °á°ú °ÅÀÇ ¸ðµç ¼¼Æ÷¿¡¼­ ½É±Ù ¸¶Ä¿ ´Ü¹éÁú cTnT°¡ ¹ßÇöÇÏ´Â ¼øµµ ³ôÀº ½É±Ù¼¼Æ÷ÀÓÀÌ È®ÀεǾú´Ù (ÁÂÃø). ¶Ç MLC2A¿Í MLC2VÀÇ ¿°»ö»ó¿¡¼­ MiraCell¢ç Cardiomyocytes (from ChiPSC12)´Â ´ëºÎºÐÀÇ ¼¼Æ÷¿¡¼­ ½É½Ç ±ÙÀ° ¼¼Æ÷ ¸¶Ä¿ÀÎ MLC2V°¡ ¹ßÇöÇÏ´Â °ÍÀ» È®ÀÎÇß°í (Áß¾Ó). ¥á-actinin¿°»öÀ¸·Î Ư¡Àû ±ÙÀý ±¸Á¶°¡ È®ÀεǾú´Ù (¿ìÃø). ±× ¿Ü °¢Á¾ À̿ Åë·Î À¯ÀüÀÚÀÇ ¹ßÇö (SCN5A, KCNQ1, CACNA1C, KCNH2µî)¿¡ ´ëÇØ¼­µµ È®ÀÎÇß´Ù (µ¥ÀÌÅÍ ºñ°ø°³).

Keyword : ½É±Ù¼¼Æ÷,½ÉÀå¼¼Æ÷,iPSÀ¯·¡¼¼Æ÷,½ÉÀ庴,µ¶¼º½ÃÇè,toxicity,toxictest,µ¶¼º

Cardiomyocyte
>
hiPS cell À¯·¡ÀÇ ½É±Ù¼¼Æ÷
ÃÊ°í¼øµµ Cardiomyocyte from human iPSC