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Home > ÀüÁ¦Ç°º¸±â > Epigenetics > Methylated DNA ºÐ¸®¿Í ºÐ¼® > EpiXplore Methylated DNA Enrichment Kit
MBD2/Magnetic Bead¸¦ ÀÌ¿ëÇÏ¿© °£´ÜÇÏ°Ô Methylated DNA¸¦ ³óÃà

EpiXplore Methylated DNA Enrichment Kit

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Clontech
631963
EpiXplore Methylated DNA Enrichment Kit
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10 ȸ
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Clontech
631962
EpiXplore Methylated DNA Enrichment Kit
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20 ȸ (631963 x 2)
(Package)
857,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631964
Magnetic Stand
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1 Set
114,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

EpiXplore Methylated DNA Enrichment Kit´Â Àüü genomeÀÇ hypo- & hyper-methylated DNA ¸ðµÎ¸¦ enriching°ú fractionating Çϱâ À§ÇÑ °£´ÜÇÑ magnetic bead ±â¹ÝÀÇ ¹æ¹ýÀÌ´Ù (Figure 1). Enrichment´Â sequencing µ¿¾ÈÀÇ background¸¦ °¨¼Ò½ÃÅ´À¸·Î À¯ÀüÀÚ Á¶Àý ¿¬±¸¿¡ ÀÖ¾î ÃÖ»óÀÇ °á°ú¸¦ °¡Á®´ÙÁØ´Ù (1).
EpiXplore Methylated Enrichment Kit¸¦ ¼±ÅÃÇØ¾ß ÇÏ´Â ÀÌÀ¯
EpiXplore protocolÀº methylated DNA¿Í ƯÀ̼ºÀÌ ³ôÀº Methylated DNA Binding (MDB) ´Ü¹éÁú »çÀÌÀÇ affinity binding¿¡ ±â¹ÝÀ» µÎ°í ÀÖ´Ù.
  • ¾î¶°ÇÑ antibodyµµ ÇÊ¿äÇÏÁö ¾Ê°í ÀÌÈÄÀÇ immunoprecipitation (IP) ÀýÂ÷°¡ ¿ä±¸µÇÁö ¾Ê´Â´Ù.
  • À¯¿¬ÇÑ ÇÁ·ÎÅäÄÝÀº (methylation ¹ÐµµÂ÷¿¡ ÀÇÇÑ) fractionÀ̳ª ÇÑ ¹øÀÇ fractionÀ¸·Îµµ methylated DNA¸¦ eluteÇϵµ·Ï ÇØÁØ´Ù.
ÀϹÝÀûÀÎ IP-±â¹ÝÀÇ enrichment ¹æ¹ý (MeDIP & MCIp)Àº À¯¿¬ÇÔÀÌ ºÎÁ·Çϱ⠶§¹®¿¡ ÇÊ¿äÇÑ Àü󸮰úÁ¤À» ÇàÇϸ鼭 ½ÃÀÛ ¹°ÁúÀÇ °¨¼Ò°¡ ¹ß»ýÇÑ´Ù. Á» ´õ Áß¿äÇÑ Á¡Àº, MeDIP & MCIpÀº methylated DNAÀÇ gradient ºÐ¸®°¡ ÀÌ·ç¾îÁöÁö ¾Ê´Â´Ù´Â Á¡ÀÌ´Ù.
¿Ö methylation ¹Ðµµ¿¡ ÀÇÇØ ºÐ¸®ÇØ¾ß Çϴ°¡?
ÃÖ±ÙÀÇ ¿¬±¸·Î ³·Àº ¹ÐµµÀÇ methylation¿¡ medium°¡ ÀÖ´Â genome ºÎºÐÀÌ º¯È­°¡ »ý±â´Â À¯ÀüÀÚ Á¶Àý¿¡¼­ Áß¿äÇÑ ¿ªÇÒÀ» ÇÑ´Ù´Â °ÍÀÌ º¸°íµÇ°í ÀÖ´Ù (2-4). EpiXplore Methylated DNA Enrichment Kit¸¸ÀÌ enriched methylated DNA°¡ methylation ¹Ðµµ¿¡ ±â¹ÝÀ» µÐ 3°³ÀÇ fractionÀ¸·Î ³ª´©¾îÁö´Â elution buffer¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.



Applications
  • Mapping methylation sites
  • Methylation-sensitive restriction enzyme-based assays
  • Real time and endpoint PCR assays
  • Bisulfite sequencing
  • Library preparation for high-throughput sequencing
  • Sample prep for DNA microarray analysis
References
1.Yegnasubramanian, S. et al. (2006) Nucleic Acids Res. 34(3):e319.

Keyword :

Methylated DNA ºÐ¸®¿Í ºÐ¼®
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