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Epigenetics
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Methylated DNA ºÐ¸®¿Í ºÐ¼®
> EpiXplore Methylated DNA Enrichment Kit
MBD2/Magnetic Bead¸¦ ÀÌ¿ëÇÏ¿© °£´ÜÇÏ°Ô Methylated DNA¸¦ ³óÃà
EpiXplore Methylated DNA Enrichment Kit
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Clontech
631963
EpiXplore Methylated DNA Enrichment Kit
10 ȸ
(Package)
451,000¿ø
Clontech
631962
EpiXplore Methylated DNA Enrichment Kit
20 ȸ (631963 x 2)
(Package)
857,000¿ø
Clontech
631964
Magnetic Stand
1 Set
114,000¿ø
Product index
Overview
[¼±Åð¡À̵å] Epigeneti..
[°³¿ä] ChIP-Seq Librar..
Cancer ¿¬±¸ °¡À̵å
DNA Methlyation ¿¬±¸
Metly-sensitive/non-se..
TaKaRa EpiTaq HS (for ..
EpiScope¢ç MSP Kit
TB Green¢ç Premix Ex T..
¸ÞÆ¿È/ºñ¸ÞÆ¿È ÄÁÆ®·Ñ..
McrBC
Methylated DNA ºÐ¸®¿Í ºÐ¼®
EpiXplore Methylated D..
Meth-Seq DNA Enrichmen..
Chromatin ¸é¿ª ħ°¨ (ChIP)
Micrococcal Nuclease
Anti-Histone monoclona..
EpiXplore ChIP Assay K..
DNA Fragmentation Kit
ChIP Elute Kit
EpiScope¢ç Nucleosome ..
Capturem¢â IP £¦ Co-IP..
ChIP-seq, Cut&Run-seq (NGS..
[Àû¿ë] ¼Ò·®¼¼Æ÷·ÎºÎÅÍ ..
[Customer Spotlight] C..
[DNA-Seq ±â¼ú°³¿ä] Thr..
[ChIP-seq ³í¹®¸®½ºÆ®] ..
SMARTer¢ç ThruPLEX¢ç D..
DNA SMART ChIP-Seq Kit
SMARTer¢ç DNA index ki..
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Canc..
MicroRNA ¿¬±¸¿ë Á¦Ç° ..
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ß..
Small RNA-Seq Library ..
MicroRNA-Seq Library P..
miRNA ¹ßÇö vector (Çü..
Epigenetics¸¦ À§ÇÑ Cloning..
Online In-Fusion Tools
Mighty TA-cloning Kit
EpiXplore Methylated DNA Enrichment Kit
´Â Àüü genomeÀÇ hypo- & hyper-methylated DNA ¸ðµÎ¸¦ enriching°ú fractionating Çϱâ À§ÇÑ °£´ÜÇÑ magnetic bead ±â¹ÝÀÇ ¹æ¹ýÀÌ´Ù (Figure 1). Enrichment´Â sequencing µ¿¾ÈÀÇ background¸¦ °¨¼Ò½ÃÅ´À¸·Î À¯ÀüÀÚ Á¶Àý ¿¬±¸¿¡ ÀÖ¾î ÃÖ»óÀÇ °á°ú¸¦ °¡Á®´ÙÁØ´Ù (1).
EpiXplore Methylated Enrichment Kit¸¦ ¼±ÅÃÇØ¾ß ÇÏ´Â ÀÌÀ¯
EpiXplore protocolÀº methylated DNA¿Í ƯÀ̼ºÀÌ ³ôÀº Methylated DNA Binding (MDB) ´Ü¹éÁú »çÀÌÀÇ affinity binding¿¡ ±â¹ÝÀ» µÎ°í ÀÖ´Ù.
¾î¶°ÇÑ antibodyµµ ÇÊ¿äÇÏÁö ¾Ê°í ÀÌÈÄÀÇ immunoprecipitation (IP) ÀýÂ÷°¡ ¿ä±¸µÇÁö ¾Ê´Â´Ù.
À¯¿¬ÇÑ ÇÁ·ÎÅäÄÝÀº (methylation ¹ÐµµÂ÷¿¡ ÀÇÇÑ) fractionÀ̳ª ÇÑ ¹øÀÇ fractionÀ¸·Îµµ methylated DNA¸¦ eluteÇϵµ·Ï ÇØÁØ´Ù.
ÀϹÝÀûÀÎ IP-±â¹ÝÀÇ enrichment ¹æ¹ý (MeDIP & MCIp)Àº À¯¿¬ÇÔÀÌ ºÎÁ·Çϱ⠶§¹®¿¡ ÇÊ¿äÇÑ Àü󸮰úÁ¤À» ÇàÇÏ¸é¼ ½ÃÀÛ ¹°ÁúÀÇ °¨¼Ò°¡ ¹ß»ýÇÑ´Ù. Á» ´õ Áß¿äÇÑ Á¡Àº, MeDIP & MCIpÀº methylated DNAÀÇ gradient ºÐ¸®°¡ ÀÌ·ç¾îÁöÁö ¾Ê´Â´Ù´Â Á¡ÀÌ´Ù.
¿Ö methylation ¹Ðµµ¿¡ ÀÇÇØ ºÐ¸®ÇØ¾ß Çϴ°¡?
ÃÖ±ÙÀÇ ¿¬±¸·Î ³·Àº ¹ÐµµÀÇ methylation¿¡ medium°¡ ÀÖ´Â genome ºÎºÐÀÌ º¯È°¡ »ý±â´Â À¯ÀüÀÚ Á¶Àý¿¡¼ Áß¿äÇÑ ¿ªÇÒÀ» ÇÑ´Ù´Â °ÍÀÌ º¸°íµÇ°í ÀÖ´Ù (2-4). EpiXplore Methylated DNA Enrichment Kit¸¸ÀÌ enriched methylated DNA°¡ methylation ¹Ðµµ¿¡ ±â¹ÝÀ» µÐ 3°³ÀÇ fractionÀ¸·Î ³ª´©¾îÁö´Â elution buffer¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
Applications
Mapping methylation sites
Methylation-sensitive restriction enzyme-based assays
Real time and endpoint PCR assays
Bisulfite sequencing
Library preparation for high-throughput sequencing
Sample prep for DNA microarray analysis
References
1.Yegnasubramanian, S. et al. (2006) Nucleic Acids Res. 34(3):e319.
Keyword :
Methylated DNA ºÐ¸®¿Í ºÐ¼®
MBD2/Magnetic Bead¸¦ ÀÌ¿ëÇÏ¿© °£´ÜÇÏ°Ô Methy..
EpiXplore Methylated DNA Enrichment Kit
¸ÞÆ¿È DNA ¼¿ ºÐ¼®À» À§ÇÑ NGS Prep kit (Ep..
Meth-Seq DNA Enrichment Kit