닫기
TaKaRa
로그인  |   회원가입  |   ID/PW 찾기   |  제품상담문의  
제품명/제품코드
키워드검색
검색
닫기
  • 고객지원 
  • 업무안내│제품문의
  • 전화번호│02-2081-2510
  • Email     │support@takara.co.kr
  • 대전지사 
  • 업무안내│대전/충청지역 주문, 제품문의
  • 전화번호│042-828-6525
  • Email     │tkbd@takara.co.kr
  • 업무시간안내
  • [ 평  일 ] 09 : 00 ~ 18 : 00 │ [ 점심시간 ] 12 : 00 ~ 13 : 00
  • 토·일요일, 공휴일은 휴무입니다.
Home > 전제품보기 > 제한효소 · Quickcut > 제한효소 > 제한효소의 Star 활성

제한효소의 Star 활성

-

제한효소의 star activity

특정 반응 조건에서 제한효소는 기질 DNA에 대한 특이성을 잃고 원래 인식부위와는 다른 염기서열을 절단하는 경우가 있다. 이러한 현상을 ‘star activity’ 라고 부르며, 효소, 기질 DNA, 반응 조건에 따라 대부분의 제한효소가 이러한 star activity를 가질 수 있다고 알려져 있다. 또한 인식 부위 이외의 서열을 절단하는 것 이외에도 ‘nicking activity’ (부분적으로 절단된 DNA)가 나타날 수도 있다.
Star activity를 억제하기 위해서는 글리세롤 농도를 낮추고, 중성 pH를 유지하며 염 농도를 높이는 조건에서 반응을 수행하는 것이 좋다. 하지만 이러한 조건으로 인해 반응성이 낮아질 수도 있다. 이러한 특정 실험 조건에서 star activity가 나타날 수 있는 제한효소와 그 경우 인식하는 서열은 아래 표에서 확인할 수 있다 (인식서열 정보 및 반응 조건은 표 하단 참조).

Restriction enzyme

Normal recognition
sequence

Reaction
conditions*

Recognition
sequence**

Reference

Aat II

GACGT↓C

E

 

Takara Bio Inc., unpublished observations

Aor13H I

T↓CCGGA

A, C, E

 

Takara Bio Inc., unpublished observations

BamH I

G↓GATCC

A, B, E, F

GRATCC
GGNTCC
GGANCC
GGATYC

Nath and Azzolina 1981; George, Blakesley, and Chirikjian 1980; George and Chirikjian 1982; Malyguine, Vannier, and Yot 1980

Ban II

GRGCY↓C

A, E, F

 

Takara Bio Inc., unpublished observations

BciT130 I

CC↓WGG

A, E,

 

 

Bgl I

GCCNNNN↓NGGC

F

 

Takara Bio Inc., unpublished observations

Bgl II

A↓GATCT

E

 

Takara Bio Inc., unpublished observations

Bln I

C↓CTAGG

B, E, G

 

 

BmeT110 I

C↓YCGRG

E

 

BspT104 I

TT↓CGAA

A, E

 

Takara Bio Inc., unpublished observations

BstP I

G↓GTNACC

A, F

 

Takara Bio Inc., unpublished observations

Bst1107 I

GTA↓TAC

F

 

Takara Bio Inc., unpublished observations

Dde I

C↓TNAG

A, B, E

 

 

EcoO65 I

G↓GTNACC

E, F

 

Takara Bio Inc., unpublished observations

EcoR I

G↓AATTC

A, B, E, F

NAATTN

Malyguine, Vannier, and Yot 1980; Polisky et al. 1975; Tikchonenko et al. 1978; Hsu and Berg 1978; Woodbury, Hagenbuchle, and von Hippel 1980

EcoR V

GAT↓ATC

E

RATATC
GNTATC
GANATC
GATNTC
GATANC
GATATY

Halford, Lovelady, and McCallum 1986; Takara Bio Inc., unpublished observations

EcoT22 I

ATGCA↓T

F, H

 

Takara Bio Inc., unpublished observations

Fba I

T↓GATCA

A, C, E, F

 

Takara Bio Inc., unpublished observations

Hae III

GG↓CC

A

 

Nath and Azzolina 1981

Hha I

GCG↓C

A, E

 

Malyguine, Vannier, and Yot 1980

Hinc II

GTY↓RAC

E

 

Takara Bio Inc., unpublished observations

Hind III

A↓AGCTT

B, E

RAGCTT
ANGCTT
AAKCTT
AAGMTT
AAGCNT
AAGCTY

Hsu and Berg 1978; Nasri and Thomas 1986; Takara Bio Inc., unpublished observations

Hpa I

GTT↓AAC

A, E

 

Nath and Azzolina 1981; Takara Bio Inc., unpublished observations

Kpn I

GGTAC↓C

A, B, C, E

 

Takara Bio Inc., unpublished observations

Mun I

C↓AATTG

A, F

 

Takara Bio Inc., unpublished observations

Nco I

C↓CATGG

A, E

 

Takara Bio Inc., unpublished observations

Nde I

CA↓TATG

A, B, G

 

 

Nhe I

G↓CTAGC

A, C, E, F

 

Takara Bio Inc., unpublished observations

Nsb I

TGC↓GCA

B

 

Takara Bio Inc., unpublished observations

PshB I

AT↓TAAT

D

 

Takara Bio Inc., unpublished observations

Psp1406 I

AA↓CGTT

E

 

Takara Bio Inc., unpublished observations

Pst I

CTGCA↓G

A, E

 

Nath and Azzolina 1981; Malyguine, Vannier, and Yot 1980; Takara Bio Inc., unpublished observations

Pvu II

CAG↓CTG

A, E

NAGCTG
CNGCTG
CANCTG
CAGNTG
CAGCNG
CAGCTN

Nasri, Sayadi, and Thomas 1985; Nasri and Thomas 1987; Takara Bio Inc., unpublished observations

RspRS II

T↓TAA

A

 

 

Sac I

GAGCT↓C

A, E

 

Takara Bio Inc., unpublished observations

Sal I

G↓TCGAC

A, E

 

Malyguine, Vannier, and Yot 1980; Takara Bio Inc., unpublished observations

Sau3A I

↓GATC

A, E

SATC
GMTC
GAKC
GATS

Pech, Streeck, and Zachau 1979; Takara Bio Inc., unpublished observations

Sca I

AGT↓ACT

B, C, F

 

Grosskopt, R. and Kessler, C., unpublished observations

Sfi I

GGCCNNNN↓NGGCC

B, E

 

Takara Bio Inc., unpublished observations

Smi I

ATTT↓AAAT

A, B, C, E

 

Takara Bio Inc., unpublished observations

Spe I

A↓CTAGT

E, F

 

Takara Bio Inc., unpublished observations

Sse8387 I

CCTGCA↓GG

E, F

 

Takara Bio Inc., unpublished observations

Ssp I

AAT↓ATT

A, C, E, F

 

Takara Bio Inc., unpublished observations

Taq I

T↓CGA

A, B, E, G

 

Takara Bio Inc., unpublished observations

Tth111 I

GACN↓NNGTC

B, C

NACNNNGTC
GNCNNNGTC
GANNNNGTC
GACNNNNTC
GACNNNGNC
GACNNNGTN

Shinomiya et al. 1982

Van91 I

CCANNNN↓NTGG

A

 

Takara Bio Inc., unpublished observations

VpaK11B I

G↓GWCC

A, C, E, F

 

Takara Bio Inc., unpublished observations

Xba I

T↓CTAGA

A, E

 

Nath and Azzolina 1981; Malyguine, Vannier, and Yot 1980


*Reaction conditions:
A: high levels of glycerol
B: in the presence of Mn2+
C: alkaline pH
D: low pH
E: in the presence of DMSO
F: low ionic strength
G: high ionic strength
H: in the presence of 2-mercaptoethanol
**Recognition sequences:
M: A or C
K: G or T
N: A or C or G or T
R: A or G
Y: C or T
W: A or T
S: G or C

References
Barany, F. The TaqI 'star' reaction: strand preferences reveal hydrogen-bond donor and acceptor sites in canonical sequence recognition. Gene 65, 149-65 (1988).
Clarke, C.M. and Hartley, B.S. Purification, properties and specificity of the restriction endonuclease from Bacillus stearothermophilus. Biochem. J. 177, 49-62 (1979).
George, J., Blakesley, R. W., Chirikjian, J. G. Sequence-specific endonuclease Bam HI. Effect of hydrophobic reagents on sequence recognition and catalysis. J. Biol. Chem255, 6521-4 (1980).
George, J. and Chirikjian, J.G. Sequence-specific endonuclease BamHI: relaxation of sequence recognition. Proc. Natl. Acad. Sci. USA 79, 2432-6 (1982).
Gingeras, T.R. and Brooks, J.E. Cloned restriction/modification system from Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 80, 402-6 (1983).
Grosskopt, R. and Kessler, C., unpublished observations.
Halford, S.E., Lovelady, B.M., Mc Callum, S.A. Relaxed specificity of the EcoRV restriction endonuclease. Gene 41, 173-81 (1986).
Heininger, K., Horz, W., Zachau, H. G., Specificity of cleavage by restriction nuclease from Bacillus subtilis. Gene 1, 291-303 (1977).
Hsu, M. and Berg, P. Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+. Biochemistry 17, 131-8 (1978).
Makula, R.A. and Meagher, R.B. A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.  Nucl. Acids Res. 8, 3125-31 (1980).
Malyguine, E. Vannier, P., Yot, P. Alteration of the specificity of restriction endonucleases in the presence of organic solvents. Gene 8, 163-77 (1980).
Nasri, M. and Thomas, D. Relaxation of recognition sequence of specific endonuclease HlndIII. Nucl. Acids Res. 14, 811-22 (1986).
Nasri, M.Sayadi, S. Thomas, D. Relaxation of PvuII recognition sequence. FEBS Lett. 185, 101-4 (1985).
Nasri, M. and Thomas, D. Alteration of the specificity of PvuII restriction endonuclease. Nucl. Acids Res. 15, 7677-87 (1987).
Nath, K. and Azzolina, B. A. Cleavage properties of site-specific restriction endonucleases. Gene Amplif. Anal. 1, 113-30 (1981).
Pech, M.Streeck, R.E., Zachau, H.G. Patchwork structure of a bovine satellite DNA. Cell 18, 883-93 (1979).
Polisky, B. et al. Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc. Natl. Acad. Sci. USA 72, 3310-4 (1975).
Shinomiya, T. et al. A new aspect of a restriction endonuclease Tth111 I. It has a degenerated specificity (Tth111 I). J. Biochem. 92, 1823-32 (1982).
Takara Bio Inc., unpublished observations.
Tikchonenko, T.I. et al. EcoRI activity: enzyme modification or activation of accompanying endonuclease? Gene 4, 195-212 (1978).
Woodbury, C.P., Hagenbuchle, O., Von Hippel, P.H. DNA site recognition and reduced specificity of the Eco RI endonuclease. J. Biol. Chem. 255, 11534-46 (1980).