Application 3) °ËÃâ °¨µµÀÇ ºñ±³ - ¼Ò·®ÀÇ Ç×ü·Îµµ ³ôÀº °¨µµÀÇ °ËÃâ °¡´É
HeLa cell lysate¸¦ ´Ü°èÀûÀ¸·Î Èñ¼®ÇÑ »ùÇÃÀ» ´Ù¾çÇÑ Èñ¼®ºñÀ²ÀÇ Ç×ü·Î Western blottingÀ» ÁøÇàÇÏ¿© GAPDH¸¦ °ËÃâÇÏ¿´´Ù.
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- Sample: HeLa cell lysate 10 §¶ºÎÅÍ ´Ü°èÀûÀ¸·Î 2¹è¾¿ Èñ¼®
- Primary antibody: anti-glyceraldehyde-3-phosphate dehydrogenase, clone 6C5 (1 §·/§¢)
- Secondary antibody: Pierce Goat Anti-Mouse IgG (H+L) Peroxidase conjugated (0.8 §·/§¢)
- Ç×üÈñ¼®ºñÀ²
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Western BLoT Hyper HRP Substrate (Code T7103A)
(³ëÃâ½Ã°£ 1ºÐ, CCD camera·Î °ËÃâ)
Western BLoT Quant HRP Substrate (Code T7102A)
(³ëÃâ½Ã°£ 1ºÐ, CCD camera·Î °ËÃâ)
Western BLoT Hyper HRP Substrate (Code T7103A) ¸¦ »ç¿ëÇÏ¿´À» ¶§,
´õ ÀûÀº ¾çÀÇ Ç×ü·Î °í°¨µµÀÇ °ËÃâÀÌ °¡´ÉÇÏ¿´´Ù.