In-Fusion technology allows easy cloning of single or multiple DNA fragments directly into large vectors (e.g., adenoviral vectors at 32.6 - 36 kb, cosmids at 46 kb*) in a single reaction, without intermediate cloning into transfer/shuttle vectors. (Please see Figures 1, 2, 5, and Table III of the Adeno-X Adenoviral System 3 Brochure for details.)

* The 46-kb cosmid vector, assembled by In-Fusion HD Cloning, was transformed in chemically competent bacteria, allowing the rescue of the recombinant vector. It was not used in the actual cosmid packaging reaction.