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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > High input DNA-seq > ThruPLEX¢ç DNA-Seq HV
FFPE, cell-free DNA-seq ºÐ¼® Á¦Ç°

ThruPLEX¢ç DNA-Seq HV

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
R400782
ThruPLEX¢ç DNA-Seq HV PLUS Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24 ȸ
(Package)
1,691,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX DNA-Seq HV PLUS User Manual-020821.pdf
Clontech
R400783
ThruPLEX¢ç DNA-Seq HV PLUS Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
5,478,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400741
ThruPLEX¢ç DNA-Seq HV
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24 ȸ
1,466,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX DNA-Seq HV User Manual_031320.pdf
Clontech
R400740
ThruPLEX¢ç DNA-Seq HV
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
4,660,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400739
ThruPLEX¢ç HV UDI 1-24
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 ȸ
518,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX HV UDI Protocol-at-a-Glance_022720.pdf
Clontech
R400738
ThruPLEX¢ç HV UDI Set A
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
1,855,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX HV UDI Protocol-at-a-Glance_022720.pdf

* ThruPLEX¢ç DNA-Seq HV PLUS Kit ³»¿¡´Â enzymatic fragmentation moduleÀ» Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.
* Á¦Ç° ³»¿¡´Â HV Àü¿ë UDI°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, Ãß°¡·Î ÇÊ¿ä½Ã¿¡¸¸ ±¸¸Å ¹Ù¶ø´Ï´Ù. HV Àü¿ëÀÌ ¾Æ´Ñ index´Â ȣȯµÇÁö ¾ÊÀ¸´Ï, Âü°í ¹Ù¶ø´Ï´Ù.


* À§ Á¦Ç°Àº high volumeÀ» À§ÇÑ Á¦Ç°ÀÔ´Ï´Ù. Low volume DNA¸¦ »ç¿ëÇÏ°íÀÚ ÇÒ ¶§¿¡´Â ¾Æ·¡ÀÇ Á¦Ç°À¸·Î »ç¿ë ¹Ù¶ø´Ï´Ù.

- Low volume DNA-seq library preparation) ThruPLEX¢ç DNA-Seq Kit (Code R400674)
ºÐ·ù

Low volume input

High volume input

input DNA

1 ~ 50 ng of DNA

5 ~ 200 ng of DNA

sample

10 §¡

30 §¡


  • »ùÇÃÀ» ³óÃàÇÒ ÇÊ¿ä ¾øÀÌ single-tube workflow·Î Illumina¢ç NGS library Á¦ÀÛ
  • Enzymatic fragmentation module Æ÷ÇÔ (300 bp, 450 bp ¼±Åà °¡´É)
  • FFPE, cfDNA 5 ~200 ng ·ÎºÎÅÍ °íÇ°ÁúÀÇ µ¥ÀÌÅÍ È¹µæ
  • High-throughput sequencer (e.g. NovaSeq¢â)À» Æ÷ÇÔÇÑ ¸ðµç ±â±â¿¡¼­ ºÐ¼® °¡´ÉÇÑ ³ôÀº ¼öÀ²ÀÇ library ȹµæ
  • ³ôÀº GC ºÎÀ§¿¡¼­µµ bias ¾øÀÌ Çâ»óµÈ ¼º´É
  • Low-frequency º¯À̸¦ ÀçÇö¼º°ú ¹Î°¨µµ ³ô°Ô NGS·Î ºÐ¼®
  • Amplification°ú sequencing ¿À·ù ÃÖ¼ÒÈ­

Á¦Ç° ¼³¸í
ThruPLEX¢ç DNA-Seq HV´Â 96 indexed library¸¦ ÀÌ¿ëÇØ Illumina¢ç NGS library¸¦ ÀÌ¿ëÇÑ multiplex ºÐ¼®À» À§ÇØ Á¦À۵Ǿú´Ù. ThruPLEX¢ç DNA-Seq HV´Â ÃÖ´ë 30 §¡ÀÇ »ùÇÃÀ» ÀÌ¿ëÇØ ³ôÀº ´Ù¾ç¼º°ú GC representationÀ» º¸À¯ÇÑ DNA library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï ÃÖÀûÈ­ µÇ¾ú´Ù. Library Á¦ÀÛÀ» À§ÇØ, 5 - 200 ngÀÇ fragmentated double-stranded DNA °¡ Àû¿ëµÉ ¼ö ÀÖ´Ù. ½ÇÇè Àü °úÁ¤Àº single tube ȤÀº single well ³»¿¡¼­ 3-stepÀ¸·Î ÁøÇàµÇ¸ç, 2½Ã°£ ³» ¿Ï·áµÈ´Ù. ½ÇÇè Áß°£¿¡ Á¤Á¦ °úÁ¤ÀÌ ÁøÇàµÇ°Å³ª, »ùÇÃÀ» ¿Å±â´Â °úÁ¤ÀÌ ºÒÇÊ¿äÇϱ⿡, ±×·Î ÀÎÇÑ »ùÇà ¼Õ½ÇÀ̳ª handling error¸¦ ¹æÁöÇÒ ¼ö ÀÖ´Ù
ThruPLEX¢ç DNA-Seq HV´Â ThruPLEX¢ç HV unique dual indexes (UDIs)¸¦ ÇÔ²² »ç¿ëÇÔÀ¸·Î½á 96-NGS-ready libraries¿¡ ´ëÇØ multiplex ºÐ¼®¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. Á¤Á¦, Á¤·®ÀÌ ¿Ï·áµÈ library´Â ±âº»ÀûÀ¸·Î »ç¿ëµÇ´Â Illumina sequencing ½Ã¾à°ú ÇÁ·ÎÅäÄÝÀ» ÀÌ¿ëÇØ Illumina NGS ±â±â¿¡¼­ ¹Ù·Î ºÐ¼® °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°Àº de novo sequencing, whole genome resequencing, whole exome sequencing ȤÀº ÀÌ¿ÜÀÇ enrichment ±â¹ýÀÇ deep sequencingÀ» À§ÇÑ ³ôÀº coverage¿Í ÇÔ²² ÃÖ°íÀÇ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. Cell-free plasma DNA ȤÀº FFPE À¯·¡ÀÇ ¼Õ»óµÈ DNA¿Í °°Àº ÀÛÀº Å©±âÀÇ DNA¿¡ °¡Àå ÀÌ»óÀûÀ¸·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.
ThruPLEX¢ç DNA-Seq HV PLUS Kit ³» Æ÷ÇԵǾî ÀÖ´Â ThruPLEX¢ç HV PLUS Enzymatic Fragmentation ModuleÀº enzymeÀ» ÀÌ¿ëÇÑ DNA fragmentation°ú repair °úÁ¤À» ÇÔ²² ¼öÇàÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÇ¾ú´Ù. µû¶ó¼­, ThruPLEX¢ç HV¸¦ ÀÌ¿ëÇÑ sequencing library Á¦ÀÛ »ùÇà Áغñ°úÁ¤¿¡¼­ º°µµÀÇ ±â±â³ª Ãß°¡ÀûÀÎ È¿¼Ò 󸮰úÁ¤ ¾øÀ̵µ ¿øÇÏ´Â ±æÀÌÀÇ DNA (300 bp, 450 bp)¸¦ ¾òÀ» ¼ö ÀÖ´Ù.


±×¸² 1. Single tube¿¡¼­ ThruPLEX¢ç DNA-Seq HV¸¦ ÀÌ¿ëÇÑ library Á¦ÀÛ °úÁ¤.
The ThruPLEX¢ç HV workflow consists of three simple steps that take place in the same well or PCR tube, eliminating the need to purify or transfer the sample material. In this latest version of the ThruPLEX technology, a higher input volume (30 ¥ìl) and range of input amounts (up to 200 ng) at the start of the protocol enables the generation of higher-complexity libraries and eliminates the need for sample concentration.


±×¸² 2. Input ¾ç°ú °ü°è¾øÀÌ ³ôÀº ÀçÇö¼º°ú µ¿ÀÏÇÑ ¾ç»óÀÇ coverage È®ÀÎ
Correlation plots are shown for replicate library preparations generated by ThruPLEX¢ç DNA-Seq HV with 5, 50, and 200 ng of EMD Millipore gDNA and downsampled to 20 million total reads. Coverage of each 10-kb region of hg19 was compared across inputs. Comparison of two independent 5-ng library preps (left), and two different starting inputs of 5 and 200 ng (right), demonstrate the high reproducibility of the system.

ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

[ThruPLEX¢ç DNA-Seq HV]
- ThruPLEX DNA-Seq HV Core Components
- ThruPLEX HV UDI 1-24 or Set A
- Control Fragmented Human gDNA (5 ng / ¥ìl)

[ThruPLEX¢ç DNA-Seq HV PLUS Kit]
- ThruPLEX HV PLUS Enzymatic Fragmentation Module Set A
- ThruPLEX DNA-Seq HV Core Components
- ThruPLEX HV UDI 1-24 or Set A
- Control Fragmented Human gDNA (5 ng / ¥ìl)

Keyword : High volume,DNA-seq,FFPE,cfDNA,cell-free DNA,DNA fragment,Enzymatic fragmentation,NGS,Next generation sequencing,NovaSeq,UDI,Unique dual index,Rubicon,·çºñÄÜ,Cancer research,¾Ï¿¬±¸,Cancer

High input DNA-seq
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FFPE, cell-free DNA-seq ºÐ¼® Á¦Ç°
ThruPLEX¢ç DNA-Seq HV
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