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Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ÀûÇÕÇÑ ¹èÁö ÷°¡Á¦

NDiff N2

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
µ¥ÀÌÅÍ°¡ ¾ø½À´Ï´Ù


  • Completely defined, serum-free formulation
  • Use with RHB-Basal medium for optimal performance
Á¦Ç°¼³¸í
NDiff N2´Â ¾Ë·ÁÁ® ÀÖ´Â ¼ººÐÀ¸·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, ¹«Ç÷û¹èÁö ÷°¡ÀÎÀÚÀÌ´Ù.
º» Á¦Ç°Àº Bottenstein Á¶¼º¿¡ ±âÃÊÇÏ°í ÀÖÀ¸¸ç (Âü°í ¹®Çå 1), ¸¶¿ì½º ES ¼¼Æ÷°¡ Mono-layer ¹è¾ç Á¶°Ç¿¡¼­ ½Å°æ ¼¼Æ÷·Î ºÐÈ­Çϵµ·Ï µÇ¾î ÀÖ´Ù (Âü°í ¹®Çå 2, 3). 
¹«Ç÷û, feeder-free culture Á¶°Ç¿¡¼­ Mouse ¹× human ½Å°æÁٱ⼼Æ÷ (NSC)¿Í ¸¶¿ì½º ES ¼¼Æ÷ÀÇ À¯µµ, À¯Áö ¹× ¹è¾ç¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
NDiff N2´Â Àν¶¸°, APO-transferrin, progesterone, putrescine°ú sodium selenite µîÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.

Product

Size

Demonstrated Applications*

NDiff N2

5 ml
(¡¿200)

  • Derivation, maintenance and expansion of mouse ES cells
  • Neural differentiation of mouse ES cells in monolayer culture
  • Derivation, maintenance and expansion of tissue-derived, adherent mouse and human NS cells
  • Differentiation of mouse and human NS cells into functional neurons

* with basal medium & supplement
³»¿ë
5 ml (¡¿200)
º¸Á¸
-20 ¡É Â÷±¤ 
(À¶ÇØ ÈÄ 4 ¡É¿¡¼­ Â÷±¤ º¸°üÇÏ°í 4ÁÖ À̳»¿¡ »ç¿ëÇÑ´Ù. ÇÑ ¹ø¿¡ ¸ðµÎ »ç¿ëÇÏÁö ¾ÊÀ¸¸é ºÐÁÖÇÏ¿© -20 ¡É¿¡¼­ Â÷±¤ÇÏ¿© º¸°üÇϸç, µ¿°á À¶Çظ¦ ¹Ýº¹ÇÏÁö ¾Ê´Â´Ù.)
ÁÖÀÇ : º» Á¦Ç°Àº ºû¿¡ ´ëÇØ ¹Î°¨Çϱ⠶§¹®¿¡ ¾îµÎ¿î °÷¿¡ º¸°üÇØ¾ß ÇÑ´Ù.
¹èÁöÁ¶Á¦ ¹æ¹ý
º» Á¦Ç°À» ½Ç¿Â¿¡¼­ Çص¿ÇÑ´Ù. ¹Ì¸® º¸¿ÂÇÑ ¹èÁö¿¡ º» Á¦Ç°À» 1/200·Î ÷°¡ÇÏ¿© (1 ¡¿ ³óµµ) ¿ÏÀüÈ÷ ±ÕÀÏÇÏ°Ô µÉ ¶§±îÁö È¥ÇÕÇÑ´Ù.
Âü°í : À§ Á¶°ÇÀº Ç¥ÁØ Á¶°ÇÀ¸·Î, ½ÇÇè ¸ñÀûÀ̳ª ¼¼Æ÷ÀÇ Á¾·ù¿¡ µû¶ó Á¶°Ç °ËÅä°¡ ÇÊ¿äÇÏ´Ù. Á¶°Ç °ËÅä½Ã¿¡´Â 1 : 400 (¡¿0.5) ~ 1 : 40 (¡¿5)ÀÇ ¹üÀ§¿¡¼­ ³óµµ¸¦ °ËÅäÇÑ´Ù.
¿ëµµ
½ÇÇè ¸ñÀû¿¡ ÇÊ¿äÇÑ Basal medium°ú supplement¸¦ ÷°¡ÇÏ¿© ÁøÇàÇÑ´Ù.
- ¸¶¿ì½º, Rat, »ç¶÷ ½Å°æÁٱ⼼Æ÷(NSC)ÀÇ À¯µµ, À¯Áö ¹× ¹è¾ç
- ¸¶¿ì½º, »ç¶÷ÀÇ mono-layer Á¶°Ç¿¡¼­ ES ¼¼Æ÷ÀÇ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­
- ¸¶¿ì½º, Rat, »ç¶÷ÀÇ ÁßÃß ½Å°æ Á¶Á÷°ú ES ¼¼Æ÷ÁÖ¿¡¼­ ½Å°æ Áٱ⠼¼Æ÷ÀÇ ºÐ¸® ¡¤ À¯µµ
- ¸¶¿ì½º, »ç¶÷ÀÇ ½Å°æÁٱ⼼Æ÷(NSC)·ÎºÎÅÍ ±â´ÉÀûÀÎ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­
»ç¿ë¿¹

±×¸² 1. NDiff N2¸¦ ÷°¡ÇÑ ¹èÁö¿¡¼­ ¸¶¿ì½º ES ¼¼Æ÷ (E14)¸¦ mono-layer ¹è¾ç ÈÄ, Tuj1 ¾ç¼º ½Å°æ ¼¼Æ÷·Î ºÐÈ­ÇÑ ¿¹


±×¸² 2. NDiff N2¸¦ ÷°¡ÇÑ ¹èÁö¿¡¼­ »ç¶÷ ½Å°æÁٱ⼼Æ÷(NSC)¸¦ mono-layer ¹è¾ç ÈÄ, Tuj1 ¾ç¼º ½Å°æ ¼¼Æ÷·Î ºÐÈ­ÇÑ ¿¹

Keyword : Cellartis,½Å°æÁٱ⼼Æ÷¹èÁö,Supplement,ESCell,¹è¾ÆÁٱ⼼Æ÷,Neuralstemcell,Neuralcell,brain,½Å°æ¼¼Æ÷ºÐÈ­

Neural Stem cell (NSC)
>
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
>
Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2