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Home > 전제품보기 > Genome Editing > CRISPR-Cas9 gene delivery > Guide-it™ Recombinant Cas9 (Electroporation-Ready)
Electroporation delivery를 위한 Cas9 단백질

Guide-it™ Recombinant Cas9 (Electroporation-Ready)


제조사 제품코드 제품명 용량 가격
비고 사용자매뉴얼
Guide-it™ Recombinant Cas9 (Electroporation-Ready)
관련학술 관심상품등록 구매하기 라이선스 
901,000원  제조사 페이지로 바로가기 122911
Guide-it™ Recombinant Cas9 (Electroporation-Ready)
관련학술 관심상품등록 구매하기 라이선스 
376,000원  제조사 페이지로 바로가기

  • The Cas9 protein solution is sterile and well-tolerated by mammalian cells
  • Highly efficient editing when combined with Guide-it Complete sgRNA Screening System (Code 632636)
  • Minimizes off-target effects-better than plasmid-based delivery systems
  • Works well in hard-to-transfect cell lines
Guide-it™ Recombinant Cas9 (Electroporation-Ready)은 Streptococcus pyogenes 유래의 Cas9 재조합 단백질로써 CRISPR/Cas9 gene editing 실험에 사용할 수 있도록 C-terminal nuclear-localization signal (NLS) 변형이 되어 있다. Cas9-sgRNA ribonucleoprotein(RNP) 복합체의 electroporation은 빠른 시간 내 gene editing이 가능하도록 하며, 또한 off-target effect를 최소화 할 수 있다.

그림 1. Gene knockouts in the Cellartis hiPSC-18 cell line. The strength of the cleaved bands gives a semiquantitive estimate of the percentage of edited cells. As can be seen, the Guide-it recombinant Cas9, when combined with sgRNA from the Guide-it In Vitro Transcription Kit, provides consistent and effective gene editing for many targets. Numbers at the bottom of each gel represent gene editing efficiencies (expressed as a %) for the indicated RNPs.

그림 2. Homology-directed repair at the AAVS1 or CXCR4 genes. A template containing a HindIII restriction site was inserted into the AAVS1 gene, and a template containing both a HindIII and a BamHI restrictrion site was inserted into the CXCR4 gene. Gene targets were then amplified from target cells using PCR and analyzed by restriction digest. Editing efficiencies are shown above each positive well on the gel. The extra band in the BamHI digest of the CXCR4 gene is due to a second BamHI site already present in the wild-type gene before editing.
관련 제품
Guide-it Complete sgRNA Screening System (Code 632636)
- Gene delivery에 적용가능한 10-20ug의 고수율, 고순도 sgRNA 합성
- sgRNA candidates 중 가장 editing 효율이 높은 sgRNA 스크리닝

Keyword : CRISPR,Cas9 protein,CRISPR/Cas9,CRISPR-Cas9,크리스퍼,gene editing,sgRNA
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Electroporation delivery를 위한 Cas9 단백질
> Guide-it™ Recombinant Cas9 (Electroporation-Ready)