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Home > 전제품보기 > DNA/RNA 정제 · 표식 > DNA/RNA 표식 > Label IT Nucleic Acid Modifying Reagent

Label IT Nucleic Acid Modifying Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 3925
Label IT AMINE Nucleic Acid Labeling Kit
관련학술 관심상품등록 구매하기
Labels 25 ug
가격문의   ml012_label_it_nucleic_acid_modifying_kit (1).pdf
Mirus
MIR 3900
Label IT AMINE Nucleic Acid Labeling Kit
관련학술 관심상품등록 구매하기
Labels 100 ug
가격문의  

  • DNA나 RNA 주형의 변형 - nucleic acid의 amine 그룹에 직접 공유 결합
  • One-step Chemical Method - nucleic acid modification의 농도(밀도)를 일관되고 쉽고 조절
  • Covalent Mechanism - 영구적이고 비파괴적인 nucleic acid modification으로 protein이나 peptide와 결합하거나 유리표면이나 bead 또는 plate에 부착 가능
Kit Components
Label IT Reagent, Label IT Reconstitution Solution, Labeling Buffer, Solutions and G50 Spin Columns.



Figure 1. The Label IT® Nucleic Acid Modifying Kit. The Label IT nucleic acid modifying reagent is composed of three regions: the reactive NH2 modifying group (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT modifying reagent to any reactive heteroatom within nucleic acids. Attachment of the Label IT modifying reagent to nucleic acids does not alter the structure of the nucleic acids or affect downstream hybridization performance. Once labeled, the nucleic acids can be coupled to reactive substrates of other chemical entities through the added NH2 reactive groups.



Figure 2. Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five μg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 - 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency.

Keyword : MIR3900, MIR3925, v3900,v3925
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