제품 설명
본 제품은 siRNA 나 plasmid 도입 확인용 제품으로 각 control은 Cy3 나 fluorescein으로 labeling 되어 있다.
in vitro and in vivo에 적용 가능하다.
Fluorophore |
Excitation Wavelength (nm) |
Emission Wavelength (nm) |
Cy®3 |
549 |
570 |
Fluorescein |
495 |
518 |
Label IT RNAi Delivery Control 적용
The Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications.
Figure 1. HeLa cells were transfected in serum-containing media with (A) Label IT® Cy®3 RNAi Delivery Control (red) using the TransIT-siQUEST® Transfection Reagent and (B) Label IT® Fluorescein RNAi Delivery Control (green) using the TransIT-TKO® Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (green).
Visualization of the Label IT® Cy®3 RNAi Delivery Control in Liver Sections Following Tail Vein Injection.
Figure 2. TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 μg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested. Sections were processed to stain the nuclei (blue) and to stain the actin (green).
Label IT Plasmid Delivery Control 적용
The Label IT® Cy®3 Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency.
Figure 3. HeLa cells were transfected in serum-containing media with the
Label IT® Cy®3 Plasmid Delivery Control (red) using the TransIT®-LT1 Transfection Reagent. Twenty-four hours post-transfection,
the cells were fixed, then counterstained to locate the nuclei (blue) and to stain the actin (green).
Visualization of Label IT® Fluorescein Plasmid Delivery Control in Liver Sections Following Tail Vein Injection.
Figure 4. TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 μg of Label IT® Fluorescein Plasmid Control (green) to a mouse using hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the actin (red).
보존
제품별 상이 (매뉴얼 참조)
