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Genome Editing
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À¯ÀüÀÚ ÆíÁý ÈÄ È®ÀÎ
> Genotype Confirmation Kit
°Ô³ðÆíÁý ÈÄÀÇ Monoallelic, biallelic indel ÆÇÁ¤¿¡
Genotype Confirmation Kit
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Clontech
632611
Guide-it¢â Genotype Confirmation Kit
100 rxns
788,000¿ø
985,000¿ø
11.01 ~ 12.27
,
,
Product index
Overivew
CRISPR/Cas9 Overview
CRISPR ½ÇÇè °¡À̵å (Tu..
About Guide-it¢â Kits
CRISPR Selection Guide
[Cancer Research] Gene..
Customer data for Guid..
Guide-it¢â À¯ÀüÀÚ ÆíÁý..
Knock-in FAQ
À¯ÀüÀÚ ÆíÁý Àü ´Ü°è
sgRNA ÇÕ¼º°ú ½ºÅ©¸®´×
[Upgrade] Guide-it¢â L..
Genome-Wide sgRNA Libr..
ÆíÁý À¯ÀüÀÚ µµÀÔ
CRISPR Plasmid System
CRISPR/Cas9 Gesicle Pr..
AAV CRISPR/Cas9 Vector..
AAV CRISPR/SaCas9 Help..
Lenti-X¢â CRISPR/Cas9 ..
Cas9 Ç×ü (Poly/Mono)
RNA Transfection Reage..
iPSC Single-Cell Cloni..
Recombinant Cas9 ´Ü¹é..
À¯ÀüÀÚ ÆíÁý ÈÄ È®ÀÎ
Mutation Detection Kit
¿°±âġȯ, »ðÀÔ º¯ÀÌ °Ë..
Genotype Confirmation ..
Indel Identification K..
CRE recombinase
CRE Recombinase Gesicl..
AAV Helper Free System..
Genome EditingÀ¸·Î »ý¼ºµÈ Monoallelic, biallelic indel µ¿Á¤
ÀçÁ¶ÇÕ Cas9 nuclease¿Í sgRNA·Î in vitro cleavage¸¦ ÀÌ¿ëÇÏ¿© ½±°Ô Genotyping
target genomic DNA¸¦ ¼¼Æ÷·ÎºÎÅÍ direct PCRÇÏ¿© Genotyping °¡´É
Á¦Ç°¼³¸í
CRISPR/Cas9 genome editing (Å©¸®½ºÆÛ °Ô³ðÆíÁý)Àº Ÿ°Ù À¯ÀüÀÚ¿¡ ÇϳªÀÇ allele (monoallelic) ¶Ç´Â µÎ°³ÀÇ allele (biallelic) º¯À̸¦ »ý¼ºÇÏ´Â °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. Guide-it Genotype Confirmation Kit (Code 632611)´Â Genome Editing ÈÄÀÇ ¼¼Æ÷ Ŭ·Ð¿¡¼ º¯ÀÌ À¯ÀüÀÚ¸¦ °£ÆíÇÏ°Ô GenotypingÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î, ÇÑÂÊ allele º¯ÀÌ (monoallelic), ´ë¸³ allele º¯ÀÌ (biallelic)¸¦ ±¸ºÐÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó, Wild type (unchanged) Ŭ·Ð±îÁö ±¸ºÐÇÒ ¼ö ÀÖ´Ù.
Cas9°ú single guide RNA (sgRNA)¸¦ ó¸®ÇÏ¿© °Ô³ðÆíÁýÀ» ½ÃÇàÇÑ ¼¼Æ÷¿¡¼ Ÿ°ÙÀ¯ÀüÀÚ¸¦ direct PCRÇÏ¿© ÁõÆøµÈ ÁõÆø»ê¹° (amplicon)À» tube ³»¿¡¼ ÀçÁ¶ÇÕ Cas9°ú sgRNA¸¦ ó¸®ÇÏ¿© in vitro cleavage¸¦ ¼öÇàÇÑ´Ù. ±× ÈÄ, Àý´Ü»ê¹°À» Agarose gel Àü±â¿µµ¿À» ½ÃÇàÇÔÀ¸·Î½á genotypeÀ» ÆÇÁ¤ÇÒ ¼ö ÀÖ´Ù.
* º» Á¦Ç°¿¡´Â sgRNA¸¦ ÇÕ¼ºÇÏ´Â ½Ã¾àÀÌ Æ÷ÇԵǾîÀÖÁö ¾Ê½À´Ï´Ù. In vitro sgRNA ÇÕ¼ºÀº
Guide-it¢â sgRNA In Vitro Transcription Kit (Code 632635)
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±×¸² 1. Guide-it Genotype Confirmation KitÀÇ ¿ø¸® ¹× Workflow.
±×¸² 2. in vitro Cas9/sgRNA Àý´Ü¿¡ ÀÇÇÑ Genotype ÆÇÁ¤
±×¸² 3. (±×¸² A) Genome EditingÀ» ¼öÇàÇÑ ¼¼Æ÷ (HEK293 Cells)ÀÇ Genotyping °á°ú (±×¸² B) ±×¸² A·ÎºÎÅÍ È¹µæ, ¼±ÅÃÇÑ Å¬·ÐÀÇ ½ÃÄö½Ì ºÐ¼® °á°ú
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CRISPR/Cas9 gene editing
Identification of monoallelic and biallelic mutations after CRISPR/Cas9 gene editing
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Extraction Buffer 1
Extraction Buffer 2
Guide-it Recombinant Cas9 Nuclease(500 ng/¥ì l)
15X Cas9 Reaction Buffer
Terra PCR Direct Polymerase Mix(1.25 U/¥ì l)
2X Terra PCR Direct Buffer (with Mg
2+
, dNTP)
RNase-Free Water
15X BSA
Monoallelic Mutant Control Fragment(20 ng/¥ì l)
Biallelic Mutant Control Fragment(20 ng/¥ì l)
Control Fragment(20 ng/¥ì l)
Control sgRNA(50 ng/¥ì l)
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Keyword : Mutation È®ÀÎ,genotyping,allele,monoalleic,bialleic,indel,NHEJ,KO,Knock out,Knockout,Knock-out,CRISPR,CRISPR/Cas9,Cas9,sgRNA,Å©¸®½ºÆÛ,gene editing,À¯ÀüÀÚÆíÁý
À¯ÀüÀÚ ÆíÁý ÈÄ È®ÀÎ
Genome editing ÀÌÈÄ mutation È®Àοë
Mutation Detection Kit
¾à 4½Ã°£¸¸¿¡ °£´ÜÇÏ°í Á¤¹ÐÇÏ°Ô »ý¼ºµÈ º¯ÀÌ ..
¿°±âġȯ, »ðÀÔ º¯ÀÌ °ËÃâ
°Ô³ðÆíÁý ÈÄÀÇ Monoallelic, biallelic indel ..
Genotype Confirmation Kit
Indel (mutation) ¼¿ È®ÀÎ
Indel Identification Kit