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Transduction Sponge
Lentivirus ½ÇÇèÀ» ÁøÇàÇÏ½Ã¸é¼ transduction È¿À²ÀÌ ³·°Å³ª µ¶¼º¿¡ ³Ê¹« ¹Î°¨ÇÑ ¼¼Æ÷¸¦ ´Ù·ïº» °æÇèÀÌ ÀÖÀ¸½Å°¡¿ä?
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Transduction Sponge¸¦ Å×½ºÆ® ÇØ º¸¼¼¿ä!!
12¿ù 27ÀϱîÁö 20% ÇÒÀÎµÈ °¡°ÝÀ¸·Î ¸¸³ªº¸½Ç ¼ö ÀÖ½À´Ï´Ù.
Lenti-X¢â Transduction Sponge (Code 631478)
- ´Ù°ø¼º ½ºÆÝÁö·Î ºü¸£°í È¿°úÀûÀ¸·Î lentivirus
transduction ÃËÁø
- SpinoculationÀ̳ª chemical ½Ã¾àÀ»
»ç¿ëÇÏÁö ¾Ê¾Æ µ¶¼ºÀ» ÃÖ¼ÒÈ
- ´Ù¾çÇÑ cell-lineÀ̳ª
primary cell µî¿¡ µ¿ÀÏÇÑ ÇÁ·ÎÅäÄÝ·Î ÃÖÀûÈ ¾øÀÌ »ç¿ë °¡´É
- 24 well plate Å©±âÀÇ 1 well¿¡¼ 105~107 cellÀ» Çѹø¿¡ ó¸® °¡´É
¡á ½ºÆÝÁö¸¦ »ç¿ëÇÑ microfluidic ¿ø¸®ÀÇ transduction enhancer
ÀÌ Á¦Ç°Àº ´Ù°ø¼ºÀÇ alginate (¾Ë±ä»ê)·Î ±¸¼ºµÈ ½ºÆÝÁöÀÔ´Ï´Ù.
½ºÆÝÁöÀÇ ÀÛÀº
´Ù°ø¼º ±¸¸ÛÀÌ microfluidic channel°ú °°Àº ¿ªÇÒÀ» ÇØ Á¼Àº °ø°£ ¾È¿¡ ¼¼Æ÷¿Í lentivirus°¡ ¹ÐÁ¢ÇØ ÀÖµµ·Ï ÇØ transductionÀ» ÃËÁøÇÕ´Ï´Ù.
±×¸²1. Lenti-X¢â
Transduction Sponge (Code 631478) ¿ø¸® ¿À¹öºä
¡á ¼ÕÀÌ ´ú°¡´Â workflow
Ÿ°Ù ¼¼Æ÷¿Í lentivirus¸¦ È¥ÇÕÇؼ ½ºÆÝÁö¿¡ Èí¼ö½ÃŲ ÈÄ CO2 ÀÎÅ¥º£ÀÌÅÍ¿¡¼ 1½Ã°£ ¹è¾ç ÈÄ ¹èÁö¸¦ ÷°¡ÇÏ¿© ´Ù½Ã 16~24½Ã°£ ¹è¾çÇÏ´Â °ÍÀ¸·Î transductionÀÌ ¸¶¹«¸®µË´Ï´Ù. ÀÌÈÄ Release Buffer·Î ½ºÆÝÁö¸¦ ³ì¿© ¼¼Æ÷¸¦ ȸ¼öÇÒ ¼ö ÀÖ½À´Ï´Ù.
(ÀÚ¼¼ÇÑ ³»¿ëÀº »ç¿ëÀÚ ¸Þ´º¾óÀ» Âü°í ºÎŹµå¸³´Ï´Ù.)