SNV, CNVÀÇ °ËÃâÀÌ °¡´ÉÇÑ Whole genome amplification (WGA) from single cells
PicoPLEX¢ç Single Cell WGA Kit v3
PicoPLEX Single Cell WGA Kit v3´Â single-tube protocol¸¦ ÀÌ¿ëÇÏ¿©, 1-10°³ÀÇ ¼¼Æ÷ ȤÀº µ¿µîÇÑ pg ¼öÁØÀÇ gDNA·ÎºÎÅÍ ÀçÇö¼º ÀÖ´Â ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Cell lysis, whole genome preamplification, ÁõÆø °úÁ¤ÀÌ 2.5½Ã°£ ³»¿¡ ÁøÇàµÇ¸ç, ÃÖ¼ÒÈµÈ ¿¡·¯¹ß»ý·ü·Î »ùÇÃÀ» 2 ¥ìg ¼öÁØÀ¸·Î ÁõÆøÇÑ´Ù. ¶ÇÇÑ High-fidelity È¿¼Ò¸¦ µµÀÔÇÔÀ¸·Î½á, ±âÁ¸ Á¦Ç°¿¡¼ °¡´ÉÇÑ CNV »Ó ¾Æ´Ï¶ó, SNV °ËÃâ¿¡µµ È°¿ëµÉ ¼ö ÀÖ´Ù.
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- Single cells ȤÀº 15 pg of DNA ¼öÁØ¿¡¼ ÀçÇö¼º ÀÖ´Â °á°ú
- ¿ùµîÇÑ Allele representation
- ÀÚµ¿È ±â±â Àû¿ë¿¡ ÃÖÀû
- ¸ðµç ½ÇÇè Àû¿ë¿¡¼ ÆíÇ⼺ ¾ø´Â °á°ú (e.g., microarray and PCR)
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Clontech |
R300718 |
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R300722 |
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R300723 |
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