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Real Time PCR È¿À²À» ³ô¿©ÁÖ°í, ½ÇÇè½Ã°£À» ´ÜÃà½ÃÄÑ ÁÙ »õ·Î¿î qRT ½Ã¾à°ú TB Green qPCR premix°¡ Ãâ½ÃµÇ¾ú½À´Ï´Ù.
1. qPCR Àü¿ë cDNA ÇÕ¼º (RT) ½Ã¾à – Á¦Ç° »ó¼¼ÆäÀÌÁö (¸µÅ©)
PrimeScript¢â FAST RT reagent Kit with gDNA Eraser
¢º gDNA Á¦°Å ¹ÝÀÀ 2ºÐ, ¿ªÀü»ç ¹ÝÀÀ 10ºÐ
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¢º Premix ½Ã¾àÀ» µü µÎ °¡Áö¸¦ ¼ø¼´ë·Î ÷°¡ÇÏ´Â
°£ÆíÇÑ Á¶ÀÛ
- #RR092A Á¦Ç° ±¸¼º (20 §¡ ¹ÝÀÀ ±âÁØ 100ȸ)
8X gDNA Eraser Premix |
200 §¡ |
5X RT Premix |
400 §¡ |
RNase Free H2O |
1 §¢¡¿2 |
EASY Dilution II (for Real
Time PCR) |
1 §¢ |
2. Intercalator TB Green °ËÃâÀ» À§ÇÑ qPCR premix – Á¦Ç° »ó¼¼ÆäÀÌÁö (¸µÅ©)
TB Green¢ç Premix Ex Taq¢â II FAST qPCR
¢º Extension time 10ÃÊÀÇ °í¼Ó¹ÝÀÀ
½ÇÇö
¢º Universal reference dye°¡
Æ÷ÇÔµÇ¾î ±â±â¿¡ µû¸¥ º°µµ ¼±ÅðúÁ¤ÀÌ ÇÊ¿ä ¾øÀ½
¢º Visualization dye (ÆĶõ»ö)°¡ Æ÷ÇÔµÇ¾î °¡½Ã¼º Çâ»ó
¢º Àú³óµµ±îÁö Á¤È®È÷ Èñ¼®ÇÏ´Â EASY
Dilution II (for Real Time PCR)ÀÌ Æ÷ÇÔµÇ¾î ½±°Ô serial
dilution °¡´É
- #RR830A Á¦Ç° ±¸¼º (25 §¡ ¹ÝÀÀ ±âÁØ 400ȸ)
TB Green Premix
Ex Taq II FAST qPCR (2¡¿) |
1
§¢
x 5 |
EASY Dilution II (for Real Time PCR) |
1
§¢
x 3 |
Á¦Ç° °ü·Ã ¹®ÀÇ ¶Ç´Â »ùÇà Å×½ºÆ®
½ÅûÀº °í°´Áö¿ø(02-2081-2510, support@takara.co.kr)À¸·Î ¿¬¶ô¹Ù¶ø´Ï´Ù.
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