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NEWS
 Á¦¸ñ : [°øÁö] mRNA ÇÕ¼º ½ÇÇè ½ºÅ¸ÅÍÆÑ!! Takara IVTpro¢â ½Ã¸®Áî
ÀÛ¼ºÀÏ : 2023.12.26
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ


 

Plasmid ÁغñºÎÅÍ in vitro transcription±îÁö Çѹø¿¡ Takara IVTpro¢â!!


´ç»ç¿¡¼­´Â mRNA ÇÕ¼º ½ÇÇèÀ» À§ÇØ IVT¿ë template plasmid Á¦ÀÛ Kit (Cloning Kit for mRNA Template (Code 6143))¿Í template plasmid¿¡¼­ ÃÖ°í ¼öÀ²·Î mRNA¸¦ ÇÕ¼ºÇÏ´Â IVT KitÀÎ (Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144))¸¦ Çϳª·Î ¹­¾î Takara IVTpro¢â mRNA Synthesis System (Code 6141)·Î ÆǸÅÇÏ°í ÀÖ½À´Ï´Ù.

ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é ¾Æ·¡ÀÇ ±×¸²°ú °°ÀÌ KitÀÇ plasmid¿¡ In-Fusion¢ç ½Ã¾àÀ¸·Î CDS (Coding sequence)¸¦ cloningÀ» ÇÏ°í ÀÌÈÄ in vitro transcriptionÀ» ÁøÇàÇØ mRNA¸¦ ÇÕ¼ºÇÑ µÚ Á¤Á¦Çϱ⠱îÁöÀÇ °úÁ¤À» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.

 


±×¸² 1. Takara IVTpro¢â ½Ã½ºÅÛ workflow 

 


±×¸² 2. Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)°ú ½ÃÁßÀÇ ´Ù¸¥ Á¦Ç°ÀÇ mRNA ÇÕ¼º ¼öÀ² ºñ±³ 



 
±×¸² 3. Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)¸¦ »ç¿ëÇÑ ´Ù¾çÇÑ ±æÀÌÀÇ mRNA ÇÕ¼º °á°ú (CleanCap + m1¥× Á¶°Ç)

 

IVT °ü·ÃÁ¦Ç°

Code

Á¦Ç°¸í

¿ëµµ

6143

Cloning Kit for mRNA Template

¨ç Template plasmid Á¦ÀÛ

6144

Takara IVTpro¢â T7 mRNA Synthesis Kit

¨è ÃÖ°í ¼öÀ² mRNA ÇÕ¼º Kit

6141

Takara IVTpro¢â mRNA Synthesis System

¨ç + ¨è

2460A

Vaccinia Capping Enzyme

Capping enzyme (uncapped -> Cap0)

2470A

mRNA Cap 2-O-Methyltransferase

Capping enzyme (Cap0 -> Cap1)

1227A

BspQ I

Template plasmid ¼±ÇüÈ­

6146

Template Vector (BspQ I) for T7 mRNA Synthesis

BspQ I¿ë IVT plasmid


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