Ç×»ó Lentivirus ³óÃàÇÏ´À¶ó Ultracentrifuge¿Í ÇÔ²² ¹ã»ùÇϽóª¿ä?
Lenti-X¢â Concentrator (Code 631232)¸¦ »ç¿ëÇϽøé ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Îµµ Çѽ𣸸¿¡ 100¹è±îÁö ³óÃàÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
Lentivirus ³óÃà ´õ ÀÌ»ó °í»ýÇϽÃÁö ¸»°í Lenti-X¢â·Î ÇÔ²²Çϼ¼¿ä.
Ư¡ 1. Mix, Wait, SpinÀÇ 3 ´Ü°è ½ÉÇÃÇÑ ÇÁ·ÎÅäÄÝ
- Lenti-X¢â Concentrator´Â ºü¸£°í °£´ÜÇϸ鼵µ ¸Å¿ì È¿°úÀûÀÎ ³óÃàÀÌ °¡´ÉÇÕ´Ï´Ù.
lentivirus¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Â ¹è¾ç »óµî¾×À» Lenti-X¢â Concentrator¿Í ¼¯¾îÁÖ°í, Àá½Ã incubation ÈÄ, ¿ø½ÉºÐ¸®ÇÏ´Â °ÍÀ¸·Î ½ÇÇèÀÌ ¸¶¹«¸® µË´Ï´Ù.
ÀÌ·± ½ÉÇÃÇÑ ÇÁ·ÎÅäÄÝ·Î ³óÃà °úÁ¤¿¡¼ ¼Õ½ÇµÇ´Â lentivirus¸¦ ÃÖ¼ÒÈÇÏ¸é¼ 1½Ã°£ ¾È¿¡ ÃÖ´ë 100¹è±îÁö ³óÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.
Ư¡ 2. 90%ÀÇ È¸¼öÀ²·Î 100¹è±îÁö ³óÃà
- Lenti-X¢â Concentrator¸¦ »ç¿ëÇϸé 107 IFU/§¢ÀÇ lentivirus¸¦ Æ÷ÇÔÇÑ ¹è¾ç »óµî¾×À» 109 IFU/§¢±îÁö, 90%ÀÇ È¸¼öÀ²·Î ³óÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.
ÀÌ·¯ÇÑ °á°ú´Â scale up ȤÀº scale down½Ã¿¡µµ µ¿ÀÏÇÕ´Ï´Ù.
Ư¡ 3. Ultracentrifugation¾øÀÌ ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Î ´õ¿í °£´ÜÇÑ »ç¿ë
- Lenti-X¢â Concentrator´Â ±âÁ¸ÀÇ ultracentrifugationÀ» »ç¿ëÇÏ´Â ¹æ¹ý°ú ºñ±³ÇØ ´õ¿í flexibleÇÏ°í ºü¸£¸ç ½¬¿î ³óÃà ¹æ¹ýÀÔ´Ï´Ù.
Feature | Lenti-X Concentrator | Ultracentrifugation |
Easily Scalable | Yes | No |
Specialized Equipment | No | Yes |
Time Required | ~1 hr | 4 hr to overnight |
Ease-of-Use | ++++ | + |
Yield | £¾90% | £¾90% |
Ư¡ 4. ´Ù¾çÇÑ ¾ç°ú ¿ª°¡ÀÇ »ùÇÿ¡ flexibleÇÏ°Ô Àû¿ë
- Lenti-X¢â Concentrator¸¦ »ç¿ëÇÏ¸é ³óÃàÇÏ°íÀÚ ÇÏ´Â ¾ç°ú ¿ª°¡¿¡ ±¸¾Ö¹ÞÁö ¾Ê°í ´Ù¾çÇÑ »ùÇÿ¡ Àû¿ë °¡´ÉÇÕ´Ï´Ù.
¾Æ·¡ÀÇ µ¥ÀÌÅ͸¦ º¸¸é 250 §¢ÀÇ »ùÇÿ¡¼µµ 95% ÀÌ»óÀÇ È¸¼öÀ²·Î 100¹è ³óÃàÇÑ °á°ú¿Í (±×¸² A), ´Ù¾çÇÑ ¿ª°¡ÀÇ »ùÇÿ¡¼ ³óÃàÇÑ °á°ú¸¦ (±×¸² B) È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
ÀÌ¿Ü lentivirus ½ÇÇèÀÌ ´õ¿í ÆíÇØÁö´Â Lenti-X¢â Á¦Ç°µé
Á¦Ç°ÄÚµå | Á¦Ç°¸í | ¿ëµµ |
632164 | Lentivirus »ý»êÀ» À§ÇÑ all-in-one ½Ã½ºÅÛ | |
632180 | °í¿ª°¡ lentivirus »ý»ê¿ë packaging cell | |
631235 | Á¤È®ÇÏ°í °£ÆíÇÑ physical lentivirus ¿ª°¡ ÃøÁ¤ | |
631476 | ||
631280 ¿Ü | ½º¸¶Æ® ÆùÀ» ÅëÇÑ 15ºÐ °£ÀÌ ¿ª°¡ ÃøÁ¤ | |
T100A/B | Àúµ¶¼º °íÈ¿À² transduction enhancer | |
631239 | Genome¿¡ »ðÀÔµÈ lentivirus (provirus) ¿ª°¡ ÃøÁ¤ |
À§ Á¦Ç°µé¿¡ ´ëÇÑ ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ Áö¿ª ´ë¸®Á¡ (´ë¸®Á¡ ¾È³») ¶Ç´Â ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
°¨»çÇÕ´Ï´Ù.