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 Á¦¸ñ : [À̺¥Æ® Á¾·á] [Ä·ÆäÀÎ] Lentivirus º£½ºÆ®¼¿·¯ Lenti-X¢â Concentrator ÇÒÀÎ Áß (~6/30)
ÀÛ¼ºÀÏ : 2023.05.23
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ

 

Second slide

Lentivirus ½ÇÇè °á°ú ¿ª°¡°¡ ±â´ëÇÑ °Í º¸´Ù ³·´Ù°í ½Ç¸ÁÇϼ̳ª¿ä?

Lenti-X¢â Concentrator (Code 631231 ¿Ü)·Î ÀÏ¹Ý ¿ø½ÉºÐ¸®±â¿¡¼­ °£´ÜÇÏ°Ô 1½Ã°£À¸·Î 100¹è±îÁö Lentivirus¸¦ ³óÃàÇؼ­ »ç¿ëÇϼ¼¿ä.

ÀÌ Á¦Ç°Àº ´ÙÄ«¶óÄÚ¸®¾Æ¿¡¼­ ÆǸŵǴ ½Ã½ºÅÛ (Lenti-X¢â, LVpro µî)À¸·Î Á¦ÀÛµÈ lentivirus ÀÌ¿Ü¿¡µµ Ÿ»ç ½Ã½ºÅÛÀ» ÀÌ¿ëÇØ Á¦ÀÛÇÑ lentivirus ¿ª½Ã Àû¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.

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Ư¡ 1. Mix, Wait, SpinÀÇ 3 ´Ü°è ½ÉÇÃÇÑ ÇÁ·ÎÅäÄÝ
- Lenti-X¢â Concentrator´Â ºü¸£°í °£´ÜÇϸ鼭µµ ¸Å¿ì È¿°úÀûÀÎ Lentivirus ³óÃà ¹æ¹ýÀÔ´Ï´Ù.

½ÇÇè°úÁ¤Àº lentivirus¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Â ¹è¾ç »óµî¾×À» Lenti-X¢â Concentrator¿Í ¼¯¾îÁÖ°í, Àá½Ã incubation ÈÄ, ¿ø½ÉºÐ¸®ÇÏ¸é µË´Ï´Ù. 

ÀÌ·± ½ÉÇÃÇÑ ÇÁ·ÎÅäÄÝ·Î ³óÃà °úÁ¤¿¡¼­ ¼Õ½ÇµÇ´Â lentivirus¸¦ ÃÖ¼ÒÈ­Çϸ鼭 1½Ã°£ ¾È¿¡ ÃÖ´ë 100¹è±îÁö ³óÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.



Ư¡ 2. 90%ÀÇ È¸¼öÀ²·Î 100¹è±îÁö ³óÃà
Lenti-X¢â Concentrator¸¦ »ç¿ëÇÏ¸é ¾Æ·¡ µ¥ÀÌÅÍ¿¡¼­ º¼ ¼ö ÀÖ´Â °Í ó·³ 107 IFU/§¢ÀÇ lentivirus¸¦ Æ÷ÇÔÇÑ ¹è¾ç »óµî¾×À» 109 IFU/§¢±îÁö, 90%ÀÇ È¸¼öÀ²·Î ³óÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.

ÀÌ·¯ÇÑ °á°ú´Â scale up ȤÀº scale down½Ã¿¡µµ µ¿ÀÏÇÕ´Ï´Ù.

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Ư¡ 3. Ultracentrifugation¾øÀÌ ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Î ´õ¿í °£´ÜÇÑ »ç¿ë
Lenti-X¢â Concentrator´Â ±âÁ¸ÀÇ ultracentrifugationÀ» »ç¿ëÇÏ´Â ¹æ¹ý°ú ºñ±³Çؼ­ ´õ¿í flexibleÇÏ°í ºü¸£¸ç ½¬¿î ³óÃà ¹æ¹ýÀÔ´Ï´Ù.

Feature

Lenti-X Concentrator

Ultracentrifugation

Easily Scalable

Yes

No

Specialized Equipment

No

Yes

Time Required

~1 hr

4 hr to overnight

Ease-of-Use

++++

+

Yield

£¾90%

£¾90%

 

Ư¡ 4. ´Ù¾çÇÑ ¾ç°ú ¿ª°¡ÀÇ »ùÇÿ¡ flexibleÇÏ°Ô Àû¿ë
Lenti-X¢â Concentrator¸¦ »ç¿ëÇÏ¸é ³óÃàÇÏ°íÀÚ ÇÏ´Â ¾ç°ú ¿ª°¡¿¡ ±¸¾Ö¹ÞÁö ¾Ê°í ´Ù¾çÇÑ »ùÇÿ¡ Àû¿ë°¡´ÉÇÕ´Ï´Ù. 

¾Æ·¡ÀÇ µ¥ÀÌÅ͸¦ º¸¸é 250 §¢ÀÇ »ùÇÿ¡¼­µµ 95% ÀÌ»óÀÇ È¸¼öÀ²·Î 100¹è ³óÃàÇÑ °á°ú¿Í (±×¸² A),

´Ù¾çÇÑ ¿ª°¡ÀÇ »ùÇÿ¡¼­ ³óÃàÇÑ °á°ú¸¦ (±×¸² B) È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.

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ÀÌ¿Ü lentivirus ½ÇÇèÀÌ ´õ¿í ÆíÇØÁö´Â Lenti-X¢â Á¦Ç°µé

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Á¦Ç°ÄÚµå

Á¦Ç°¸í

¿ëµµ

632164

Lenti-X¢â Expression System

Lentivirus »ý»êÀ» À§ÇÑ all-in-one ½Ã½ºÅÛ 

632180

Lenti-X¢â 293T Cell Line

°í¿ª°¡ lentivirus »ý»ê¿ë packaging cell

631235

Lenti-X¢â qRT-PCR Titration Kit

Á¤È®ÇÏ°í °£ÆíÇÑ physical lentivirus ¿ª°¡ ÃøÁ¤

632200

Lenti-X¢â p24 Rapid Titer Kit

631280 ¿Ü

Lenti-X¢â GoStix¢â Plus

½º¸¶Æ® ÆùÀ» ÅëÇÑ 15ºÐ °£ÀÌ ¿ª°¡ ÃøÁ¤

T100A/B

RetroNectin¢ç

Àúµ¶¼º °íÈ¿À² transduction enhancer

631239

Lenti-X¢â Provirus Quantitation Kit

Genome¿¡ »ðÀÔµÈ lentivirus (provirus) ¿ª°¡ ÃøÁ¤

À§ Á¦Ç°µé¿¡ ´ëÇÑ ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ Áö¿ª ´ë¸®Á¡ (´ë¸®Á¡ ¾È³») ¶Ç´Â ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.

 

°¨»çÇÕ´Ï´Ù. 

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