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event
  : [θ] Cloning, NGS, typing , long range PCR ְ (~4/19 !)
ۼ : 2024.04.01
 ۼ : īڸ

Long range PCR ְ, PrimeSTAR GXL DNA Polymerase

 

Cloning, NGS, typing !

پ ̿ GC contents ȿ ϴ long range PCR ְ,

PrimeSTAR GXL DNA Polymerase 20% ΰ ϼ.

-      θ Ⱓ:  2024 4 19 ()

 

  • High Fidelity PCR ȿ ǥ
  • (elongation factor) ÷Ͽ long fragment (human gDNA ~30 kb)
  • GC rich target ȿ
  • Anti-Taq antibody hot start PCR

 

Code

ǰ

Ư¡

R050A/B

PrimeSTAR GXL DNA Polymerase

250 U/1,000 U

ȿ, buffer, dNTP , hot-start

R051A/B

PrimeSTAR GXL Premix

200 ȸ/800 ȸ

2x premix, hot-start

R052A/B

PrimeSTAR GXL Premix Fast, Dye plus

200 ȸ/800 ȸ

2x premix (dye plus),

Fast PCR (ð~10), hot-start

*ذ Ȯ PCR ȿ 

PrimeSTAR Max DNA Polymerase Ver.2 (Code R047A/B) 20% !

ǰ Ȯ ûϱ (ũ)

 

 

*Բ ϸ TA-cloning kit: 20% (~4/19)

Code

ǰ

Ư¡

High fidelity PCR ȿҷ insert ϰ TA cloning

6019

Mighty TA-cloning Reagent Set for PrimeSTAR

20ȸ

A-tailing þ, ligation premix,

T-vector

ʰ ligation 1 solution premix

6023

DNA Ligation Kit Mighty Mix

1 Kit

Ligation ʿ þ Ե premix



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