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event
 Á¦¸ñ : [À̺¥Æ® Á¾·á] [ÇÁ·Î¸ð¼Ç] TCR/BCR profilingÀº ÀÌÁ¦ NGS°¡ ´ë¼¼ÀÔ´Ï´Ù (20% ÇÒÀÎ Áß)
ÀÛ¼ºÀÏ : 2024.11.25
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ

TCR/BCR ºÐ¼®ÀÇ ÃÖ¼±µÎ ±â¼ú NGS Immune profiling kit


TCR (T-cell receptor)°ú BCR (B-cell receptor)Àº ´Ù¾çÇÑ Ç׿øÀ» ÀνÄÇÒ ¼ö ÀÖµµ·Ï À¯ÀüÀÚ Àç¹è¿­ ¹× µ¹¿¬º¯À̸¦ ÅëÇØ ³ôÀº °¡º¯¼ºÀ» °¡Áö°í ÀÖ°í À̸¦ TCR/BCR Repertoire¶ó°í ÇÕ´Ï´Ù. À̸¦ ºÐ¼®ÇÏ´Â °ÍÀ» immune profilingÀ̶ó Çϴµ¥ Takara Bio Á¦Ç°Àº Immune profiling¿¡¼­ 5¡¯-RACE ±â¼úÀ» »ç¿ëÇϱ⠶§¹®¿¡ ±âÁ¸ÀÇ multiplex PCRÀ» »ç¿ëÇÏ´Â ¹æ¹ý°ú ´Þ¸® PCR bias°¡ Àû°í, SMART-Seq¢ç ±â¼úÀ» »ç¿ëÇϱ⠶§¹®¿¡ ±Ø¼Ò·®ÀÇ »ùÇÿ¡¼­µµ ¸Å¿ì ³ôÀº Á¤È®µµ·Î full-lengthÀÇ NGS library¸¦ Á¦ÀÛÇÏ¿© ºÐ¼®À» ÁøÇàÇÕ´Ï´Ù.


 

 

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¡á   TCR-¥á , TCR-¥â chain (TCR Á¦Ç°È¤Àº ¸ðµç BCR heavy chain (BCR Á¦Ç°)ÀÇ V(D)J¿µ¿ªÀ» ³²±è¾øÀÌ full-length·Î °ËÃâ

¡á   UMI (Unique Molecular Identifier) ±â¹ÝÀ¸·Î PCR duplicate, error º¸Á¤

¡á   CDR3 ¿µ¿ª¸¸ ¼±ÅÃÀûÀ¸·Î ºÐ¼®ÇÏ´Â °Í ¿ª½Ã °¡´É

¡á   UDI (Unique Dual Index)°¡ »ç¿ëµÇ¾î multiplexing °¡´É

 

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NGS±â±â

SMART-Seq¢ç Human TCR (with UMIs) (Code 634780)

PBMCs, T cells, whole blood µî RNA

- V(D)J ºÐ¼®:  Illumina¢ç MiSeq¢â

- CDR3 ºÐ¼®: Illumina¢ç ¸ðµç Ç÷§Æû

SMART-Seq¢ç Mouse TCR (with UMIs) (Code 634815)

Spleen, T cells, whole blood, bone marrow, thymus µî

SMART-Seq¢ç Human BCR (with UMIs) (Code 634777)

PBMCs, B cells, spleen, bone marrow, whole blood µî

SMART-Seq¢ç Mouse BCR (with UMIs) (Code 634352)

PBMCs, spleen, bone marrow, lymph nodes, whole blood µî

 

°ü·Ã ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ Áö¿ª ´ë¸®Á¡ (´ë¸®Á¡ ¾È³»¶Ç´Â °í°´Áö¿ø (02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.  


÷ºÎÆÄÀÏ: °í°¨µµ TCR_BCR profiling ºÐ¼®À» À§ÇÑ SMART-Seq NGS ¼Ö·ç¼Ç.pdf
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