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[Legacy] NGS Products
> (Legacy) Human TCR a/b Profiling Kit
TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ Full-length sequence °¡´É
(Legacy) Human TCR a/b Profiling Kit
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[Legacy] NGS Products
(Legacy) Human TCR a/b..
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* º» Á¦Ç°Àº ÃֽŹöÀüÀÎ
SMART-Seq
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Human TCR (with UMIs)
(Code 634779-634781)·Î ¾÷±×·¹ÀÌµå µÇ¾ú½À´Ï´Ù.
* ÇϳªÀÇ ¿¬±¸ ÇÁ·ÎÁ§Æ®¿¡´Â µ¿ÀÏÇÑ ¹öÀüÀÇ Á¦Ç°À» »ç¿ëÇϽô °ÍÀ» ±ÇÀåµå¸³´Ï´Ù.
TCR-alpha, TCR-beta chain
À» µ¿½Ã¿¡ ¶Ç´Â °³º°ÀûÀ¸·Î NGS Library Á¦ÀÛ °¡´É
Complete V(D)J sequence information
: T-cell receptor (TCR)ÀÇ V(D)J °¡º¯¿µ¿ª (V(D)J variable regionÀÇ Full-length sequencing
Flexible sample inputs
: ¼Ò·®ÀÇ Human blood RNA sample (10ng - 3ug) ¶Ç´Â intact lymphocytes (50 - 10,000 purified T cells)¿¡¼ NGS Library¸¦ Á¦ÀÛ °¡´É
Illumina-ready sequencing libraries
: ligation-independent ¹æ½ÄÀ¸·Î illumine adaptor ¹× index sequence ºÎÂø °¡´É
Exceptional sensitivity, and excellent reproducibility
Á¦Ç°¼³¸í
SMARTer Human TCR a/b Profiling KitÀº T-cell receptor (TCR) repertoireÀÇ NGS ºÐ¼®À» À§ÇÑ Illumina platform NGS Library Á¦ÀÛ Å°Æ®ÀÌ´Ù. º» Á¦Ç°Àº ¹Ì·®ÀÇ Human blood RNA sample (10ng - 3ug), ¶Ç´Â intact lymphocytes (50 - 10,000 purified T cells)·ÎºÎÅÍ TCR Àü»çüÀÇ V(D)J °¡º¯¿µ¿ª (variable region) ºÐ¼®À» À§ÇÏ¿© 5¡¯ RACE À¯»ç ±â¼úÀ» Á¢¸ñÇÏ¿´À¸¸ç, TCR mRNAÀÇ Full length sequence¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé TCR-¥á, TCR-¥â chainÀ» µ¿½Ã¿¡ ¶Ç´Â chain¿¡ ´ëÇÑ ¶óÀ̺귯¸®¸¦ Á¦ÀÛÇÏ¿© ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
Multiplexing PCRÀ» ÀÌ¿ëÇÏ´Â ±âÁ¸ÀÇ TCR profiling ¹æ¹ý°ú ´Þ¸®, º» Á¦Ç°Àº °¢°¢ÀÇ TCR-¥á¿Í TCR-¥â chain¿¡ ´ëÇÑ single primer¸¦ »ç¿ëÇÏ´Â 2´Ü°èÀÇ PCR ¹æ¹ýÀ¸·Î Illumina¿ë ¶óÀ̺귯¸®¸¦ ÁõÆøÇÑ´Ù.
º» Á¦Ç°Àº LNA ±â¼úÀÌ °áÇÕµÈ SMART (
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echanism
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emplate)¹ýÀ» ÀÌ¿ëÇϹǷΠ¹Î°¨µµ°¡ ¸Å¿ì ³ô°í ÆíÇ⼺ÀÌ ÀûÀº TCR mRNA sequence ºÐ¼®À» ÇÒ ¼ö ÀÖ´Ù.
±×¸² 1. Protocol overview and timeline
±×¸² 2. SMARTer TCR profiling ±â¼úÀÇ Library Á¦ÀÛ °úÁ¤ ¹× ¿ø¸®
Panel A. TCR subunit mRNAÀÇ ¿ªÀü»ç¹ÝÀÀ°ú PCR ÁõÆø °úÁ¤
Panel B. TCR-¥á, TCR-¥â chainÀÇ ÁõÆøÀ» À§ÇÑ semi-nested PCR
±×¸² 3. TCR sequencing libraryÀÇ Electropherogram profiles
Panel A.
Typical Fragment Analyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from peripheral blood leukocyte RNA
Panel B.
Typical Bioanalyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from peripheral blood leukocyte RNA (same library as Panel A)
Panel C
. Typical Fragment Analyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from monoclonal Jurkat T-cell RNA.
Panel D.
Typical Bioanalyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from Jurkat T-cell RNA (same library as Panel C)
Application
Human TCR repertoire analysis (TCR-alpha and TCR-beta subunits)
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer Human TCR a/b Profiling Kit (Code 635014)
SMARTer Human TCR a/b Profiling Kit Components (Cat. No. 635017; Not sold separately)
TCR a/b Human Indexing Primer Set HT for Illumina - 12 (Cat. No. 635020; Not sold separately)
SeqAmp DNA Polymerase (Cat. No. 638504)
Keyword : TCR,CDR3,T cell receptor,repertoire,variable region,Immune cell,Immune-seq,Immunology,,SMART,smart-seq,NGS,Next generation sequencing,cancer,Cancer research,¾Ï¿¬±¸,vaccine,¹é½Å,¹é½Å¿¬±¸
[Legacy] NGS Products
TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ Full-length seque..
(Legacy) Human TCR a/b Profiling Kit
Single cell, 10pg DNA - Whole Genome Amplifi..
(Legacy) PicoPLEX¢ç WGA Kit