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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Beta cell > Beta-cell from human iPSC
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Beta-cell from human iPSC

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Cellartis
Y10100
Cellartis¢ç hiPS Beta Cells (from ChiPSC12) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
°¡°Ý¹®ÀÇ   Cellartis_Beta_Cells_User_Manual_020218.pdf
Cellartis
Y10106
Cellartis¢ç hiPS Beta Cells (from ChiPSC22) Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
°¡°Ý¹®ÀÇ  


  • Àν¶¸°/ C-Peptide, MAFA, NKX6.1, PDX1, UCN3 µîÀÇ ÃéÀå ¥â¼¼Æ÷ ¸¶Ä¿¸¦ °í¹ßÇöÇÔ.
  • iPS¼¼Æ÷¿¡¼­ ³»¹è¿± ºÐÈ­¸¦ °ÅÃÄ ÃéÀå ¼¼Æ÷(ÃéÀå ¥â ¼¼Æ÷)·Î ºÐÈ­µÊ.
  • ¥â ¼¼Æ÷ ±â´É¿¬±¸¿Í ½Å¾à °³¹ß ½ºÅ©¸®´× µîÀÇ in vitro ½ÃÇè¿¡ ÃÖÀû
  • GSIS(glucose-stimulated insulin secretion)¿¬±¸³ª incretin ³óµµ ÀÇÁ¸ÀûÀÎ Àν¶¸° ºÐºñ ´É·Â º¸À¯
  • ChipPSC22 À¯·¡ ¥â ¼¼Æ÷´Â 1Çü ´ç´¢º´ Ãë¾à ´ë¸³À¯ÀüÀÚ HLA-A*0201¸¦ °¡Áö°í ÀÖ´Ù
  • ISSCR2017 Poster: Advanced diabetes drug discovery¸¦ À§ÇÑ hiPSC-derived beta cells (Ŭ¸¯)
Á¦Ç°¼³¸í
Cellartis hiPS Beta Cells(from ChiPSC12)Àº Àΰ£ iPS¼¼Æ÷·ÎºÎÅÍ in vitroÀÇ ³»¹è¿± ºÐÈ­¸¦ °ÅÃÄ À¯µµÇÑ ÃéÀå ¥â ¼¼Æ÷·Î »ýü³» Ç÷´ç Á¶Àý°ú ´ç´¢º´ ¹ßº´¿¡ ±íÀÌ °ü¿©ÇÏ´Â Àν¶¸°À̳ª C-peptideÀÇ ¹ßÇöÀº ¹°·Ð MAFA, UCN3, NKX6.1, PDX1 µîÀÇ ¼º¼÷ ÃéÀå ¥â ¼¼Æ÷ ¸¶Ä¿¸¦ ¹ßÇöÇÑ´Ù. µû¶ó¼­ ÇöÀç Àΰ£ ÃéÀå ¥â ¼¼Æ÷ÀÇ ´ë¿ëÀ¸·Î ³Î¸® »ç¿ëµÇ°í ÀÖ´Â ¼³Ä¡·ù¿Í °°Àº µ¿¹° ÃéÀå ¼¼Æ÷ À¯·¡ ¥â ¼¼Æ÷´ë½Å ÃéÀå ¥â ¼¼Æ÷ ±â´É ÇØ¼®À̳ª ´ç´¢º´/Áúº´¸ðµ¨, ±×¸®°í Àν¶¸° ºÐºñ¿Í Á¶Àý¿¡ °ü¿©ÇÏ´Â ¹°ÁúÀÇ °Ë»ç µî¿¡ ÀÌ¿ë °¡´ÉÇÏ´Ù.
º» Á¦Ç°Àº Cellartis DEF-CS 500 Culture System(Code Y30010)À» ÀÌ¿ëÇÏ¿© freeder free Á¶°ÇÀ¸·Î ¹è¾çµÈ human iPS ¼¼Æ÷ÁÖ Cellartis human iPS cell line 12(ChiPSC12)(Code Y00285)¸¦ ÀÌ¿ëÇß´Ù.

º» Á¦Ç°Àº Cellartis hiPS Beta Cells(from ChiPSC12)µ¿°á¼¼Æ÷ÀÇ ÇØµ¿°ú ¹è¾ç¿¡ ÇÊ¿äÇÑ ¹èÁö(Cellartis Beta Cell Basal Medium, Cellartis Beta Cell Basal Medium 2, Cellartis Beta Cell Supplement) ¹× ¹è¾ç¿ë ÄÚÆÃÁ¦(Cellartis Beta Cell Coating)°¡ Æ÷ÇԵǾî ÀÖ´Ù.
³»¿ë

Cellartis hiPS Beta Cells (from ChiPSC12)

1 vial 4.8¡¿106 viable cells

Cellartis Beta Cell Supplement

360 ¥ìl¡¿11

Cellartis Beta Cell Coating

4 ml

Cellartis Beta Cell Basal Medium

160 ml

Cellartis Beta Cell Basal Medium 2

30 ml

¼ö¼Û
-80 ¡É
º¸Á¸
  • Cellartis hiPS Beta Cells(from ChiPSC12) : ¾×ü Áú¼Ò º¸Á¸
    µµÂø ÈÄ °ð¹Ù·Î ¾×üÁú¼Ò ÅÊÅ©¿¡ º¸°üÇϼ¼¿ä.
    ¾×ü Áú¼Ò º¸Á¸ÀÌ ¾ÈµÉ °æ¿ì ¹Ù·Î ¼¼Æ÷¸¦ À¶ÇØÇÏ¿© ¹è¾çÇϼ¼¿ä.
  • Cellartis Beta Cell Basal Medium, Cellartis Beta Cell Basal Medium 2 :
    -20¡É ÀÌÇÏ, À¶ÇØ ÈÄ 4¡É º¸Á¸
    -20¡É ÀÌÇÏ¿¡¼­ µ¿°á º¸Á¸ÇÏ°í »ç¿ë Àü¿¡ 2~8¡É¿¡¼­ O/NÀ¸·Î ÇØµ¿À» Çϼ¼¿ä.
    À¶ÇØ ÈÄ¿¡´Â 2~8¡É¿¡¼­ º¸Á¸Çϰí 1°³¿ù À̳» »ç¿ëÇϼ¼¿ä(´Ù½Ã µ¿°á À¶ÇØ´Â ºÒ°¡).
  • Cellartis Beta Cell Supplement : -20¡É ÀÌÇÏ, Â÷±¤ º¸Á¸, »ç¿ë Á÷Àü¿¡ ÇØµ¿Çϼ¼¿ä.
  • Cellartis Beta Cell Coating:-20¡É ÀÌÇÏ º¸Á¸, »ç¿ë Á÷Àü¿¡ ÇØµ¿Çϼ¼¿ä.



±×¸² 1. Cellartis hiPS Beta Cells (from ChiPSC12) were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).



±×¸² 2. Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose. The content of secreted C-peptide was analyzed using the Mercodia C-peptide ELISA kit. The results are mean ¡¾ SEM (n=5, GLP-1; n=4, exenatide). *p<0.05.



±×¸² 3. Immunostaining of Cellartis hiPS Beta Cells (from ChiPSC12) for insulin and C-peptide. Immunostaining of cells was performed to detect maturation markers insulin (Panel A) and C-peptide (Panel B) two weeks post-thaw. Merged data illustrates co-localization of insulin and C-peptide expression (Panel C). Cell nuclei were stained with DAPI (Panel D).

Keyword : ´ç´¢,ÃéÀå¼¼Æ÷,º£Å¸¼¼Æ÷,Betacell,insulin,Àν¶¸°,pancreas,GSIS,Glucose-stimulatedinsulinsecretion

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