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GFP Variant Vector

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
632312
pGFPuv Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
835,200¿ø 
1,044,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33372
Clontech
632370
pGFP Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
835,200¿ø 
1,044,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33371

* ÇØ´ç Á¦Ç°Àº 3¡¯ MCS¿¡ stop codonÀ» Æ÷ÇÔÇÏ°í ÀÖ¾î, cloning ½Ã¿¡´Â 5¡¯ MCS¸¦ ÀÌ¿ëÇÏ½Ç °ÍÀ» ±ÇÀå µå¸³´Ï´Ù.

ZsGreen1
ZsGreenÀº Anthozoa-class »êÈ£ÃʷκÎÅÍ À¯·¡µÈ ¾ÆÁÖ ¹àÀº tetramer green fluorescent proteinÀÌ¸ç ¸Å¿ì ¶Ù¾î³­ reporterÀÌ´Ù (EGFPº¸´Ù 2.5 ¹è ÀÌ»ó ¹àÀ½. Table I).
AcGFP1
AcGFP1Àº ÇØÆĸ® Aequorea coerulescens¿¡¼­ À¯·¡µÈ monomeric green fluorescent proteinÀÌ´Ù. ClontechÀº fusion, reporter, subcellular localizationÀ» À§ÇÑ AcGFP1 vector¿Í ±×¿¡ ´ëÇÑ source¸¦ Á¦°øÇÏ°í ÀÖ´Ù.
GFP Variant Vector
ÀÌ ¹ÚÅ׸®¾Æ ¹ßÇö vector´Â GFP (wild type Aequorea victoria green fluorescent protein) ¶Ç´Â GFPuv (UV¿¡ ÀÇÇØ ÀÚ±ØÀ» ¹ÞÀ» ¶§ ÃÖ°íÀÇ Çü±¤À» ³ªÅ¸³»µµ·Ï ÃÖÀûÈ­µÈ GFP º¯ÀÌü) Áß ÇϳªÀÇ À¯ÀüÀÚ¸¦ °¡Áö°í ÀÖÀ¸¸ç, lac promoter¿¡ ÀÇÇØ À¯µµµÈ´Ù. ¶ÇÇÑ, ÀÌ vector´Â Á¦½ÃµÈ fluorescent proteinÀÇ cDNA sequence¸¦ ¾òÀ» ¼ö ÀÖ´Â Àç·á·Î »ç¿ë °¡´ÉÇÏ´Ù.

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