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100pg DNA·ÎºÎÅÍ ÆíÇ⼺ ¾ø´Â Preamplification (for qPCR, NGS, genotyping)

PreAmp Master Mix

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(ºÎ°¡¼¼º°µµ)
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Clontech
638541
Prelude¢â PreAmp Master Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
40ȸ (638542 x 4)
962,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x105961
Clontech
638542
Prelude¢â PreAmp Master Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
10ȸ
267,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • Least-biased preamplification - Ÿ»çÀÇ preamplification Á¦Ç°¿¡ ºñÇÏ¿© ÀÌ»óÁ¡ (outlier)ÀÌ 2 ~ 3¹è ´õ ÀûÀº ÆíÇ⼺ÀÌ ¸Å¿ì ³·Àº ÁõÆø °¡´É
  • Greatest sensitivity - ÃÖ¼Ò 100pg cDNA ¶Ç´Â gDNA·ÎºÎÅÍ ÁõÆø
  • Universal compatibility - ÁõÆøµÈ »ùÇà (preamplified sample)Àº Real Time PCR (100°³ÀÇ intercalating dye¹ý, ¶Ç´Â dual-labeled probe¹ý), SNP genotyping ¶Ç´Â target enriched NGS¿¡ Àû¿ë
  • Convenient, ready-to-use master mix - Master mix ŸÀÔÀ¸·Î cDNA¿Í gDNA »ùÇÃ, Primer set Ãß°¡ ÈÄ PCR ¹ÝÀÀ (10 - 14 Cycles)
Á¦Ç°¼³¸í
Takara PreAmp Master Mix´Â 2X PCR mix ÇüÅÂÀÇ Á¦Ç°À¸·Î, ¾ÆÁÖ ÀûÀº ¾çÀÇ cDNA ¶Ç´Â gDNA (100pg - 10ng)·ÎºÎÅÍ 100°³ ÀÌ»óÀÇ Å¸°Ù¿¡ ´ëÇØ ÆíÇ⼺ ¾øÀÌ preamplificationÀÌ °¡´ÉÇÑ PCR È¿¼ÒÀÌ´Ù. º» Á¦Ç°À¸·Î ÁõÆøµÈ »ê¹°Àº Real Time PCR (qPCR), Targeted enrichment, ¶Ç´Â genotyping ¸ñÀûÀ¸·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.
Preamplification ½ÇÇèµ¥ÀÌÅÍ
PrimerArray Cell Cycle (Human) panel¿¡ Æ÷ÇÔµÈ 96°³ÀÇ Å¸°Ù À¯ÀüÀÚ¿¡ ´ëÇØ Takara PreAmp Master Mix·Î preamplificationÇÑ °á°ú¸¦ ³ªÅ¸³»¾ú´Ù. ƯÁ¤ À¯ÀüÀÚ¿¡ ´ëÇؼ­¸¸ xÃà¿¡ ¸í¸íÇÏ¿´À¸¸ç, Àüü Ÿ°Ù À¯ÀüÀÚ ¸®½ºÆ®´Â ¿©±â¿¡¼­ È®ÀÎ °¡´ÉÇÏ´Ù. Ct°ªÀÌ 32¸¦ ÃÊ°úÇÏ´Â nompreamplified gene¿¡ ´ëÇؼ­´Â Ç¥±âµÇÁö ¾Ê¾Ò´Ù (±×¸² 1 - ±×¸² 6).


±×¸² 1. The benefits of preamplification.


±×¸² 2. Uniform representation following preamplification.


±×¸² 3. Uniformity following either 10 or 14 cycles of preamplification.


±×¸² 4. Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles.


±×¸² 5. Sensitive, unbiased preamplification using as little as 12.5 pg of starting material


±×¸² 6. Takara PreAmp Master Mix provides less biased preamplification than other master mixes

FFPE »ùÇ÷κÎÅÍ 162°³ÀÇ À¯¹æ¾Ï À¯ÀüÀÚ¿¡ ´ëÇØ Takara PreAmp Master Mix¿Í TaqMan¢â PreAmp Master Mix·Î preamplification ÈÄ ºÐ¼®ÇÑ °á°ú, Takara PreAmp Master Mix°¡ º¸´Ù ÆíÇ⼺ÀÌ ÀûÀº ºÐ¼® °á°ú¸¦ º¸¿´´Ù (±×¸² 7).



±×¸² 7. Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶ ¹Ù¶ø´Ï´Ù)
  • Takara PreAmp Master Mix (Code 638541)          4 x 250 §¡
  • Takara PreAmp Master Mix (Code 638542)          1 x 250 §¡
º¸Á¸ - 20¡ÆC

Keyword :

qPCR Preamplification
>
100pg DNA·ÎºÎÅÍ ÆíÇ⼺ ¾ø´Â Preamplification..
PreAmp Master Mix