- High-throughput single-cell analysis-generate sequencing data from >1,000 single cells in parallel
- Multiplex for higher throughput-use SMART-Seq ICELL8 indexing primer sets A and B on two chips, and then pool the samples to allow sequencing of >2,000 cells simultaneously
- Sample flexibility-analyze cells from up to eight different samples in a single experiment
- Reduced sample handling-minimize variability with automated cell isolation and selection, and sample pooling prior to sequencing-ready library construction
- Illumina sequencing libraries-generate libraries suitable for sequencing on all Illumina platforms
ICELL8 cx Single Cell SystemÀ¸·Î ºÐ¸®µÈ single cellÀº ºó 5,184 nano wellÀÇ 350V chip(Code 640019)À¸·Î ºÐÁֵǰí, CellSelect Software¸¦ »ç¿ëÇÏ¿© single cellÀÌ ºÐÁֵǾî ÀÖ´Â wellÀ» ½Äº°,
¼±ÅÃÇÑ ÈÄ SMART RT-PCR ½Ã¾àÀ» ¼±ÅÃÇÑ well¿¡ Ãß°¡·Î ºÐÁÖÇÑ´Ù. single cell°ú RT-PCR ½Ã¾àÀÌ ºÐÁÖµÈ chipÀº ICELL8 cx Thermal cycler·Î ¿Å°Ü¼ 1st strand cDNAÀÇ ÇÕ¼º°ú ÁõÆøÀ» À§ÇØ one-step
RT-PCRÀÌ ÀÌ·ïÁö°Ô µÈ´Ù. ÀÌ·¸°Ô ÁغñµÈ full-length cDNA´Â TDE1(Illumina Tagment DNA Enzyme)¿¡ ÀÇÇØ tag°¡ ÁöÁ¤µÇ°í, ÀÌÈÄ forward ¹× reverse indexing primer(Code 640205 ¹× 640218)¸¦
»ç¿ëÇÏ¿© ÁõÆøµÈ´Ù. ÀÌ¿Í °°ÀÌ Á¦ÀÛµÈ library¸¦ ȸ¼ö, Á¤Á¦ ¹× ÁõÆøÇÏ¿© ÃÖÁ¾ sequencingÀÌ °¡´ÉÇÑ library¸¦ ÁغñÇÒ ¼ö ÀÖ´Ù.