닫기
TaKaRa
로그인  |   회원가입  |   ID/PW 찾기   |  제품상담문의  
제품명/제품코드
키워드검색
검색
닫기
  • 고객지원센터 
  • 업무안내│학술지원
  • 전화번호│02-2081-2510
  • Email     │support@takara.co.kr
  • 대전지사 
  • 업무안내│대전/충청지역 주문, 학술지원
  • 전화번호│042-828-6525
  • Email     │tkbd@takara.co.kr
  • 업무시간안내
  • [ 평  일 ] 09 : 00 ~ 18 : 00 │ [ 점심시간 ] 12 : 00 ~ 13 : 00
  • 토·일요일, 공휴일은 휴무입니다.
Home > 전제품보기 > Cloning 관련 > In-Fusion Cloning > Large/Small Insert Cloning

Large/Small Insert Cloning

-

Large/Small Insert Cloning



1. Large Insert Cloning
In-Fusion technology has been optimized for cloning large fragments.
DNA inserts up to 15 kb have been successfully cloned into pUC19 using In-Fusion Cloning.


그림. Ten out of ten colonies contain the correct insert (100% cloning efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

2. Small Insert Cloning
The smallest insert successfully cloned with In-Fusion Cloning was a 50-bp synthetic oligonucleotide (including two 15-nt homologous overlaps with the vector termini).
For In-Fusion Cloning of short synthetic oligos (between 50 and 150 bp), the suggested oligo-to-vector molar ratio is 5-15:1. Depending on oligo length, the optimal ratio must be determined empirically.

Note: Non-phosphorylated oligonucleotides are compatible with In-Fusion Cloning. However, the 3' exonuclease activity in the In-Fusion enzyme mix requires terminal 3' OH groups.