• Results you can trust. ThruPLEX^® Tag-seq technology provides up to 16 million molecular tags to correct sequencing errors providing confident variant detection.
• Discover more, cost effectively. Use with hybridization-based target enrichment systems to examine hundreds of genes, including mutations and structural variants, simultaneously.
• High quality libraries the first time, every time. Unparalleled ease of use reduces user error and contamination with our single-tube, 2-hour, 3-step workflow.
• Time-saving bioinformatics solutions. Reach results quickly with cloud-based software from Curio Genomics or open-source scripts available for data processing.
• Precious samples go further. Use less DNA to analyze samples once too low to detect. ThruPLEX^® Tag-seq works with input amounts from 1 ng to 50 ng of cfDNA or fragmented dsDNA to accommodate a wide range of samples.

제품설명

보관

내용

Component name	Cap Color	6S Kit 6 Single Indexes	48S Kit 48 Single Indexes	96D Kit 96 Dual Indexes
Code		RB4584	RB4585	RB4586
용량		12회	48회	96회
Template Preparation Buffer	Red	1 Tube	1 Tube	2 Tubes
Template Preparation Enzyme	Red	1 Tube	1 Tube	2 Tubes
Library Synthesis Buffer	Yellow	1 Tube	1 Tube	2 Tubes
Library Synthesis Enzyme	Yellow	1 Tube	1 Tube	2 Tubes
Library Amplification Buffer	Green	1 Tube	1 Tube	2 Tubes
Library Amplification Enzyme	Green	1 Tube	1 Tube	2 Tubes
Nuclease-Free Water	Clear	1 Tube	1 Tube	1 Tube
Indexing Reagents	Blue	6 Tubes	1 Single Index Plate (48S)	1 Dual Index Plate (96D)

※ ThruPLEX^® Tag-seq Kit single Index 서열
   * ThruPLEX Tag-seq 6S Kit (RB4584)
   * ThruPLEX Tag-seq 48S Kit (RB4585)
※ ThruPLEX^® Tag-seq Kit Index Guide 바로 가기


[그림1] Single-Tube, Three step workflow helps your precious samples go further
Starting with 1 to 50 ng of DNA, ThruPLEX^® Tag-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.


[그림 2] ThruPLEX^® Technology generates high quality libraries the first time, every time
ThruPLEX^® technology is a 3-step reaction that starts with fragmented double-stranded DNA or cell-free DNA which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5' ends reduce adapter-adapter ligation.


[그림 3] Reduce background for results you can trust
ThruPLEX^® Tag-seq libraries were prepared from 30 ng of the 1% Horizon cfDNA standard. Following Agilent SureSelect target enrichment and sequencing to achieve ~10,000X coverage, data was analyzed with the Curio Genomics bioinformatics platform. Using the raw reads, the EGFR mutation (yellow dot) is obscured by background errors (left). The mutation is readily identified when UMTs are utilized in data processing to correct for errors (right).


[그림 4] Dramatically increase the signal to noise ratio to discover more
Data from either the 30 ng Horizon cfDNA library(A) or a ThruPLEX^® Tag-seq library prepared from 10 ng Horizon cfDNA standard, enriched with a custom 240 KB panel (NimbleGen SeqCapEZ) and sequenced on a HiSeq 2500 to achieve ~5,000X coverage. Analysis was performed using the Curio Genomics bioinformatics platform.