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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > [Legacy] NGS Products > (Legacy) ThruPLEX¢ç Tag-seq Kit
Illumina¢ç NGS Library Preparation with Unique Molecular Tags

(Legacy) ThruPLEX¢ç Tag-seq Kit

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* Àá±ñ, ù ±¸¸ÅÀΰ¡¿ä? ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (Tel. 02-2081-2510)·Î ¿¬¶ôÁÖ¼¼¿ä!
  • Results you can trust. ThruPLEX¢ç Tag-seq technology provides up to 16 million molecular tags to correct sequencing errors providing confident variant detection.
  • Discover more, cost effectively. Use with hybridization-based target enrichment systems to examine hundreds of genes, including mutations and structural variants, simultaneously.
  • High quality libraries the first time, every time. Unparalleled ease of use reduces user error and contamination with our single-tube, 2-hour, 3-step workflow.
  • Time-saving bioinformatics solutions. Reach results quickly with cloud-based software from Curio Genomics or open-source scripts available for data processing.
  • Precious samples go further. Use less DNA to analyze samples once too low to detect. ThruPLEX¢ç Tag-seq works with input amounts from 1 ng to 50 ng of cfDNA or fragmented dsDNA to accommodate a wide range of samples.
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ThruPLEX¢ç Tag-seq KitÀº molecular tag¿Í ThruPLEX chemistry¸¦ °áÇÕ½ÃÄÑ, »ùÇà index°¡ ºÎ°¡µÈ Illumina NGS library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù.
ThruPLEX¢ç Tag-seq KitÀÇ Stem-Loop adaptor´Â 1600¸¸ °³ÀÇ unique molecular tag(UMT)À» Æ÷ÇÔÇϰí À־, PCR ÁõÆø Àü¿¡ °¢°¢ÀÇ DNA ´ÜÆí¿¡ UMTÀ» ºÎ°¡ ÇÒ ¼ö ÀÖ´Ù. ±× °á°ú, NGS µ¥ÀÌÅÍ ºÐ¼®¿¡¼­ À§¾ç¼º ºñÀ²À» °¨¼Ò½ÃÄÑ º¸´Ù Á¤È®ÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
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Store at -20 ¡ÆC.
Guarantee for 9 months at -20¡ÆC in a constant temperature freezer.
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Component name

Cap Color

6S Kit
6 Single Indexes

48S Kit
48 Single Indexes

96D Kit
96 Dual Indexes

Code

¡¡

RB4584

RB4585

RB4586

¿ë·®

¡¡

12ȸ

48ȸ

96ȸ

Template Preparation Buffer

Red

1 Tube

1 Tube

2 Tubes

Template Preparation Enzyme

Red

1 Tube

1 Tube

2 Tubes

Library Synthesis Buffer

Yellow

1 Tube

1 Tube

2 Tubes

Library Synthesis Enzyme

Yellow

1 Tube

1 Tube

2 Tubes

Library Amplification Buffer

Green

1 Tube

1 Tube

2 Tubes

Library Amplification Enzyme

Green

1 Tube

1 Tube

2 Tubes

Nuclease-Free Water

Clear

1 Tube

1 Tube

1 Tube

Indexing Reagents

Blue

6 Tubes

1 Single Index Plate (48S)

1 Dual Index Plate (96D)


¡Ø ThruPLEX¢ç Tag-seq Kit single Index ¼­¿­
   * ThruPLEX Tag-seq 6S Kit (RB4584)
   * ThruPLEX Tag-seq 48S Kit (RB4585)
¡Ø ThruPLEX¢ç Tag-seq Kit Index Guide ¹Ù·Î °¡±â


[±×¸²1] Single-Tube, Three step workflow helps your precious samples go further
Starting with 1 to 50 ng of DNA, ThruPLEX¢ç Tag-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.


[±×¸² 2] ThruPLEX¢ç Technology generates high quality libraries the first time, every time
ThruPLEX¢ç technology is a 3-step reaction that starts with fragmented double-stranded DNA or cell-free DNA which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5' ends reduce adapter-adapter ligation.


[±×¸² 3] Reduce background for results you can trust
ThruPLEX¢ç Tag-seq libraries were prepared from 30 ng of the 1% Horizon cfDNA standard. Following Agilent SureSelect target enrichment and sequencing to achieve ~10,000X coverage, data was analyzed with the Curio Genomics bioinformatics platform. Using the raw reads, the EGFR mutation (yellow dot) is obscured by background errors (left). The mutation is readily identified when UMTs are utilized in data processing to correct for errors (right).


[±×¸² 4] Dramatically increase the signal to noise ratio to discover more
Data from either the 30 ng Horizon cfDNA library(A) or a ThruPLEX¢ç Tag-seq library prepared from 10 ng Horizon cfDNA standard, enriched with a custom 240 KB panel (NimbleGen SeqCapEZ) and sequenced on a HiSeq 2500 to achieve ~5,000X coverage. Analysis was performed using the Curio Genomics bioinformatics platform.

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