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Stem Cell
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Mouse stem cell °ü·Ã Á¦Ç°
> NDiff N2
Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ÀûÇÕÇÑ ¹èÁö ÷°¡Á¦
NDiff N2
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hiPSC/hESC
½Å¾à°³¹ßÀ» À§ÇÑ iPSC ..
DEF-CS¢â Overview: ÇÑ ..
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[»ç¿ë Èıâ] Â÷¼¼´ë ÁÙ..
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Xeno-Free 3D Spheroid ..
Áٱ⼼Æ÷ À¯ÀüÀÚÆíÁý
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iPSC Single-Cell Cloni..
MSC (Mesenchymal Stem Cell..
Human MSC xeno-free cu..
Mesenchymal progenitor..
Hepatocyte
[Àû¿ë] hiPS-HEP cells ..
[Àû¿ë] hiPS-HEP cells ..
Primary hepatocyte med..
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Beta cell
Definitive Endoderm ce..
hiPSC to DE(definitive..
Neural Stem cell (NSC)
½Å°æÁٱ⼼Æ÷ ¿¬±¸ÀÇ To..
[Àû¿ë] NDiff 227À» ÀÌ..
RHB-A/RHB Basal
NDiff N2
NDiff277
Endothelial Cell
Endothelial Cells from..
iPS Cell to Endothelia..
Mouse stem cell °ü·Ã Á¦Ç°
[Àû¿ë] NDiff 227À» ÀÌ..
Cellartis¢ç 3i mESC/iP..
Cellartis¢ç 2i mESC/iP..
RHB-A/RHB Basal
NDiff227
NDiff N2
Osteoblast-Inducer Rea..
iPS cell Á¦ÀÛ ¹× °ËÃâ
Stem antibodies
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Coating matrix
Easy iMatrix-511 / Eas..
iMatrix-511 / iMatirx-..
Completely defined, serum-free formulation
Use with RHB-Basal medium for optimal performance
Á¦Ç°¼³¸í
NDiff N2´Â ¾Ë·ÁÁ® ÀÖ´Â ¼ººÐÀ¸·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, ¹«Ç÷û¹èÁö ÷°¡ÀÎÀÚÀÌ´Ù.
º» Á¦Ç°Àº Bottenstein Á¶¼º¿¡ ±âÃÊÇÏ°í ÀÖÀ¸¸ç (Âü°í ¹®Çå 1), ¸¶¿ì½º ES ¼¼Æ÷°¡ Mono-layer ¹è¾ç Á¶°Ç¿¡¼ ½Å°æ ¼¼Æ÷·Î ºÐÈÇϵµ·Ï µÇ¾î ÀÖ´Ù (Âü°í ¹®Çå 2, 3).
¹«Ç÷û, feeder-free culture Á¶°Ç¿¡¼ Mouse ¹× human ½Å°æÁٱ⼼Æ÷ (NSC)¿Í ¸¶¿ì½º ES ¼¼Æ÷ÀÇ À¯µµ, À¯Áö ¹× ¹è¾ç¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
NDiff N2´Â Àν¶¸°, APO-transferrin, progesterone, putrescine°ú sodium selenite µîÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Product
Size
Demonstrated Applications*
NDiff N2
5 ml
(¡¿200)
Derivation, maintenance and expansion of mouse ES cells
Neural differentiation of mouse ES cells in monolayer culture
Derivation, maintenance and expansion of tissue-derived, adherent mouse and human NS cells
Differentiation of mouse and human NS cells into functional neurons
* with basal medium & supplement
³»¿ë
5 ml (¡¿200)
º¸Á¸
-20 ¡É Â÷±¤
(À¶ÇØ ÈÄ 4 ¡É¿¡¼ Â÷±¤ º¸°üÇÏ°í 4ÁÖ À̳»¿¡ »ç¿ëÇÑ´Ù. ÇÑ ¹ø¿¡ ¸ðµÎ »ç¿ëÇÏÁö ¾ÊÀ¸¸é ºÐÁÖÇÏ¿© -20 ¡É¿¡¼ Â÷±¤ÇÏ¿© º¸°üÇϸç, µ¿°á À¶Çظ¦ ¹Ýº¹ÇÏÁö ¾Ê´Â´Ù.)
ÁÖÀÇ : º» Á¦Ç°Àº ºû¿¡ ´ëÇØ ¹Î°¨Çϱ⠶§¹®¿¡ ¾îµÎ¿î °÷¿¡ º¸°üÇØ¾ß ÇÑ´Ù.
¹èÁöÁ¶Á¦ ¹æ¹ý
º» Á¦Ç°À» ½Ç¿Â¿¡¼ Çص¿ÇÑ´Ù. ¹Ì¸® º¸¿ÂÇÑ ¹èÁö¿¡ º» Á¦Ç°À» 1/200·Î ÷°¡ÇÏ¿© (1 ¡¿ ³óµµ) ¿ÏÀüÈ÷ ±ÕÀÏÇÏ°Ô µÉ ¶§±îÁö È¥ÇÕÇÑ´Ù.
Âü°í : À§ Á¶°ÇÀº Ç¥ÁØ Á¶°ÇÀ¸·Î, ½ÇÇè ¸ñÀûÀ̳ª ¼¼Æ÷ÀÇ Á¾·ù¿¡ µû¶ó Á¶°Ç °ËÅä°¡ ÇÊ¿äÇÏ´Ù. Á¶°Ç °ËÅä½Ã¿¡´Â 1 : 400 (¡¿0.5) ~ 1 : 40 (¡¿5)ÀÇ ¹üÀ§¿¡¼ ³óµµ¸¦ °ËÅäÇÑ´Ù.
¿ëµµ
½ÇÇè ¸ñÀû¿¡ ÇÊ¿äÇÑ Basal medium°ú supplement¸¦ ÷°¡ÇÏ¿© ÁøÇàÇÑ´Ù.
- ¸¶¿ì½º, Rat, »ç¶÷ ½Å°æÁٱ⼼Æ÷(NSC)ÀÇ À¯µµ, À¯Áö ¹× ¹è¾ç
- ¸¶¿ì½º, »ç¶÷ÀÇ mono-layer Á¶°Ç¿¡¼ ES ¼¼Æ÷ÀÇ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ
- ¸¶¿ì½º, Rat, »ç¶÷ÀÇ ÁßÃß ½Å°æ Á¶Á÷°ú ES ¼¼Æ÷ÁÖ¿¡¼ ½Å°æ Áٱ⠼¼Æ÷ÀÇ ºÐ¸® ¡¤ À¯µµ
- ¸¶¿ì½º, »ç¶÷ÀÇ ½Å°æÁٱ⼼Æ÷(NSC)·ÎºÎÅÍ ±â´ÉÀûÀÎ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ
»ç¿ë¿¹
±×¸² 1. NDiff N2¸¦ ÷°¡ÇÑ ¹èÁö¿¡¼ ¸¶¿ì½º ES ¼¼Æ÷ (E14)¸¦ mono-layer ¹è¾ç ÈÄ, Tuj1 ¾ç¼º ½Å°æ ¼¼Æ÷·Î ºÐÈÇÑ ¿¹
±×¸² 2. NDiff N2¸¦ ÷°¡ÇÑ ¹èÁö¿¡¼ »ç¶÷ ½Å°æÁٱ⼼Æ÷(NSC)¸¦ mono-layer ¹è¾ç ÈÄ, Tuj1 ¾ç¼º ½Å°æ ¼¼Æ÷·Î ºÐÈÇÑ ¿¹
Keyword :
Mouse stem cell °ü·Ã Á¦Ç°
[Àû¿ë] NDiff 227À» ÀÌ¿ëÇÑ mouse organoid Á¦ÀÛ
Mouse ESC/iPSC ¹è¾ç¹èÁö (3i technology)
Cellartis¢ç 3i mESC/iPSC Culture Medium
Mouse ESC/iPSC ¹è¾ç¹èÁö (2i Technology)
Cellartis¢ç 2i mESC/iPSC Culture Medium
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
¸¶¿ì½º ES ¼¼Æ÷ ½Å°æºÐȹèÁö
NDiff227
Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2
°ñ¾Æ¼¼Æ÷ ºÐÈ ½Ã¾à
Osteoblast-Inducer Reagent (for animal cell)