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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Mouse stem cell °ü·Ã Á¦Ç° > Cellartis¢ç 3i mESC/iPSC Culture Medium
Mouse ESC/iPSC ¹è¾ç¹èÁö (3i technology)

Cellartis¢ç 3i mESC/iPSC Culture Medium

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Cellartis
Y40011
Cellartis¢ç 3i mES/iPSC Culture Medium
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 §¢
°¡°Ý¹®ÀÇ   Y40011_e.v2002.pdf

  • Feeder-free, cytokine-free, serum-free »óÅ¿¡¼­ °­·ÂÇÑ ¡®Ground State (Naive state)¡¯¸¦ À¯ÁöÇϸç Mouse ESC/iPSC ¹è¾ç
  • Mouse ESC È®¸³ÀÌ ¾î·Á¿î Á¾ (¿¹: C57BL/6N Mouse)¿¡¼­µµ ÁÁÀº °á°ú ¾òÀ½
  • Cellartis¢ç 3i mES/iPSC Culture Medium¿¡¼­ ¹è¾çµÈ mouse ESC´Â LIF¿Í serum ¶Ç´Â BMP ÷°¡µÈ »óÅÂ¿Í ºñ±³ÇßÀ» ¶§ 2¹è ³ôÀº ¼ºÀå·ü À¯Áö
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Cellartis¢ç 3i mES/iPSC Culture MediumÀº 3Á¾·ùÀÇ small molecule inhibitor (ERK/MEK inhibitor, GSK3 ¥â inhibitor, FGFR-TK inhibitor)¸¦ »ç¿ëÇÏ¿©, mouse ESC/iPSC Á¦ÀÛ, À¯Áö ¹× È®´ë¹è¾çÇϱâ À§ÇØ Á¦Á¶µÈ defined, serum free ¹è¾ç ¹èÁöÀÌ´Ù. 3Á¾·ùÀÇ inhibitor°¡ Æ÷ÇԵǾî ÀÖ¾î, mouse ESCÀÇ ºÐÈ­ À¯µµ ½ÅÈ£¸¦ Á¦¾îÇÏ°í ¼¼Æ÷ ¼ºÀåÀ» ÃËÁøÇϸç, cytokine ¾øÀ̵µ pluripotency¿Í ground state¸¦ °­·ÂÇÏ°Ô À¯ÁöÇÑ´Ù. µû¶ó¼­, Cellartis¢ç 3i mES/iPSC Culture Medium´Â pluripotencyÀÇ molecular mechanism ¿¬±¸¿¡ »ç¿ë °¡´ÉÇÏ´Ù.
Cellartis¢ç 3i mES/iPSC Culture Medium´Â ÀϹÝÀûÀÎ Á¶°Ç¿¡¼­ mouse ESCÀÇ È®¸³ÀÌ ¾î·Á¿ü´ø mouse Á¾¿¡ Àû¿ë °¡´ÉÇÏ´Ù. 1
Cellartis¢ç 3i mES/iPSC Culture Medium¿¡¼­ ¹è¾çµÇ´Â mouse ESC´Â Leukemia Inhibitory Factor (LIF)¿Í serum ¶Ç´Â Bone Morphogenetic Protein (BMP) 1, 2, 3¿¡¼­ ¹è¾çµÈ ¼¼Æ÷¿Í ºñ±³ÇßÀ» ¶§ 2¹èÀÇ ¼ºÀå ¼Óµµ¿Í ´õ ³ôÀº pluripotency¸¦ °¡Áø colony¸¦ Çü¼ºÇÑ´Ù.
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  • Cellartis mES/iPSC Culture Basal Medium (Code Y40005, 200 §¢; º°µµ±¸¸Å ºÒ°¡)
  • Cellartis 3i mES/iPSC Supplement (Code Y40012, 200 §¡; º°µµ±¸¸Å ºÒ°¡)
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  • Cellartis mES/iPSC Culture Basal Medium : -20 ¡É
  • Cellartis 3i mES/iPSC Supplement : -80 ¡É
»ç¿ë»ó ÁÖÀÇ»çÇ×
  • °í¿Â ´Ù½ÀÇÑ È¯°æÀ» ÇÇÇÏ°í, Â÷±¤ÇÏ¿© º¸°üÇϽʽÿÀ.
  • Çص¿ ÈÄ Basal medium¿¡ Supplement¸¦ Àü·® ÷°¡ÇÏ°í, 4 ¡É¿¡ º¸°üÇϸç 2ÁÖÀÏ À̳»¿¡ »ç¿ëÇϽʽÿÀ. (Do not refreeze after thawing)
  • »ç¿ëÀü Á¦Á¶ÇÑ Cellartis¢ç 3i mES/iPSC Culture MediumÀº »ç¿ëÇÒ ¾ç¸¸ ¼ÒºÐÇÏ¿© µû¶æÇÏ°Ô ÇÑ ÈÄ »ç¿ëÇϽʽÿÀ. (warm this aliquot to between room temperature and 37¡É)
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1. Ying, Qi-Long, et al. "The ground state of embryonic stem cell self-renewal." Nature 453.7194 (2008): 519-523.

2. Kiyonari, Hiroshi, et al. "Three inhibitors of FGF receptor, ERK, and GSK3 establishes germline-competent embryonic stem cells of C57BL/6N mouse strain with high efficiency and stability." genesis 48.5 (2010): 317-327.

3. Iijima, Saori, et al. "Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice." Cellular Reprogramming (Formerly" Cloning and Stem Cells") 12.6 (2010): 679-688.

Keyword :

Mouse stem cell °ü·Ã Á¦Ç°
>
Mouse ESC/iPSC ¹è¾ç¹èÁö (3i technology)
Cellartis¢ç 3i mESC/iPSC Culture Medium
>
Mouse ESC/iPSC ¹è¾ç¹èÁö (2i Technology)
Cellartis¢ç 2i mESC/iPSC Culture Medium
>
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
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¸¶¿ì½º ES ¼¼Æ÷ ½Å°æºÐÈ­¹èÁö
NDiff227
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Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2
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°ñ¾Æ¼¼Æ÷ ºÐÈ­ ½Ã¾à
Osteoblast-Inducer Reagent (for animal cell)