ÀÓ»ó¿ë lentivirus, ¾ÈÀü°ú È¿À²À» ÃÖ´ë·Î
Receptor ±â¹Ý transduction enhancer (GMPµµ º¸À¯)
RetroNectin¢ç (Code T100A ¿Ü)
- Lentivirus / RetrovirusÀÇ transduction enhancer·Î RetroNectinÀÇ Heparin-binding domainÀÌ ¹ÙÀÌ·¯½º¿Í °áÇÕÇÑ µÚ Cell-binding domain°ú ¼¼Æ÷°¡ °áÇÕÇØ transductionÀ» ÃËÁøÇÑ´Ù. ÈçÈ÷ »ç¿ëÇÏ´Â chemical ±â¹ÝÀÇ polybrene, protamine°ú ºñ±³ÇØ
µ¶¼ºÀÌ ¸Å¿ì ³·°í È¿À²ÀÌ ³ô¾Æ ±âÁ¸¿¡´Â ¹ÙÀÌ·¯½º µµÀÔÀÌ ¾î·Á¿ü´ø suspension cell, primary cell, stem cellÀÇ transduction È¿À²À» ³ôÀ̴µ¥ ³Î¸® »ç¿ëµÇ°í ÀÖ½À´Ï´Ù.
- T-cell È®´ë¹è¾ç¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ¾î CAR-T ¿¬±¸¿¡µµ ÃÖÀûÀÇ Á¦Ç°ÀÔ´Ï´Ù. (
T-cell È®´ë¹è¾ç ¼Ö·ç¼Ç).
HIV ¼¿À» ±Øµµ·Î ÁÙ¿© ÀÓ»ó½ÇÇè¿¡ ÃÖÀûȽÃŲ 3¼¼´ë lentivirus ½Ã½ºÅÛ
LVpro¢â Packaging Mix (pLVpro Vector) (Code 6962 ¿Ü)
- HIV-1 À¯·¡ÀÇ tat ¼¿ÀÌ Á¦°ÅµÇ°í, 3¡¯ LTRÀÇ ÀϺμ¿ °á¼ÕÀ¸·Î ¹ÙÀÌ·¯½ºÀÇ ÀÚ°¡ º¹Á¦¸¦ ¹æÁöÇÏ´Â µî (self-inactivation; SIN) 3¼¼´ë lentivirusÀÇ Æ¯Â¡À» °¡Áö°í ÀÖ½À´Ï´Ù. ƯÈ÷
HIV-1 À¯·¡ ¼¿À» ÇÑ°èÄ¡±îÁö Á¦°ÅÇØ ¾ÈÀü¼ºÀ» ³ô¿© ÀÓ»ó ¿¬±¸¿¡ ÃÖÀûÀÎ Á¡ÀÌ Æ¯Â¡ÀÔ´Ï´Ù. Lentivirus »ý»ê¿¡ ÇÊ¿äÇÑ ¸ðµç packaging plasmid (
env,
gag,
pol,
rev ¹ßÇö)¸¦ premix ÇüÅ·ΠÁ¦°øÇÏ¿© °£´ÜÇÏ°Ô ½ÇÇèÀ» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
Cell & Gene therapy ¿¬±¸¸¦ ÇÑ´Ù¸é,
RetroNectin¢ç°ú LVpro¢â, ÀÌ ÃÖ°íÀÇ Á¶ÇÕÀ» ¼±ÅÃÇØ ÁÖ¼¼¿ä.
*Âü°íÀÚ·á
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Lentivirus ¼±Åð¡À̵å
*°ü·Ã Á¦Ç°
- ½ÇÇè ÈÄ ÀÜ·ùÇÑ RetroNectin
¢çÀ» ELISA·Î È®ÀÎ
RetroNectin¢ç EIA Kit (Code MK140)
- RetroNectin
¢çÀÇ GMP grade ¹öÀü
RetroNectin¢ç GMP grade (Code T202)
- 1step qRT-PCR·Î ºü¸£°í Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
- 1.5½Ã°£À¸·Î ºü¸£°Ô ³¡³ª´Â ELISA¹ý ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash) (Code 631476)
- Lentivirus ³óÃàÀ» ÀϹݿø½ÉºÐ¸®±â·Î 1½Ã°£¸¸¿¡
Lenti-X¢â Concentrator (Code 631231)