No cold room, No buffer exchange
Capturem¢â
His-Tagged Purification kit ½Ã¸®Áî
- Â÷¼¼´ë Capturem¢â membrane ±â¼úÀ» Àû¿ëÇÑ His-tagged protein Á¤Á¦
- ½Ç¿Â¿¡¼ ~30ºÐ Á¤Á¦ ¿Ï·á
: ¹è¾ç¾×¿¡¼ buffer ±³È¯ ¾øÀÌ Á÷Á¢ ºÐºñÇü(secreted) ´Ü¹éÁú Á¤Á¦ °¡´É - application º¸±â (¸µÅ©)
- ºñº¯¼º ¹× º¯¼º Á¶°Ç (EDTA, ȯ¿øÁ¦, ±Û¸®¼¼·Ñ µî)¿¡¼µµ Á¤Á¦ °¡´É
±âÁ¸ÀÇ metal affinity resin¿¡ ºñÇÏ¿© º¸´Ù ´Ù¾çÇÑ »ùÇà (¹ÚÅ׸®¾Æ, Æ÷À¯·ù Àû¿ë °¡´É)°ú
´Ù¾çÇÑ Á¶°Ç (ºñº¯¼º ¶Ç´Â º¯¼º Á¶°Ç (8M urea, 6M
Guanidine-HCl)¿¡¼ Àû¿ëÇÒ ¼ö ÀÖ´Â Capturem¢â His-Tagged
Purification kit ½Ã¸®Á 20% ÇÒÀΰ¡·Î ¸¸³ªº¸¼¼¿ä.
- ÀÚ¼¼ÇÑ Á¦Ç°Á¤º¸ ¹× ÇÒÀΰ¡ È®ÀÎÇϱâ (¸µÅ©)
|
Miniprep
Kit |
Maxiprep
Kit |
96well
plate |
24well
plate |
Large
volume |
Code |
635710 |
635713 |
635714 |
635730 |
635724 |
ÇüÅ |
Miniprep
Column |
Maxiprep
column |
96
Nickel plate |
24
Nickel plate |
Large
volume unit |
Input sample |
~
800 §¡ |
~
25 §¢ |
~
1,000 §¡ |
~
4 §¢ |
150
~ 500 §¢ |
¿ë·® |
20
ȸ |
6
ȸ |
96
well |
24
well |
4
ȸ |
[ÇÔ²² »ç¿ëÇϸé ÁÁÀº ´Ü¹éÁú Á¤Á¦ °ü·Ã Á¦Ç°]
- °¢ Á¦Ç°¸íÀ» Ŭ¸¯ÇϽøé ÀÚ¼¼ÇÑ Á¦Ç° Á¤º¸ ¹× ÇÒÀΰ¡¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
Ư¡ |
Á¦Ç°¸í |
¿ë·® |
Code |
Á»´õ ³ôÀº ¼øµµ·Î Á¤Á¦ |
10 §¢ |
635501 | |
°í¼öÀ² Á¤Á¦¿¡ ÃÖÀû |
10 §¢ |
635659 | |
Æí¸®ÇÑ lysis buffer set |
250 §¢ |
635671 | |
ETDA free, Æí¸®ÇÑ »ç¿ë |
ProteoGuard¢â EDTA-Free Protease Inhibitor Cocktail |
100 §¡ |
635672 |
Á¦Ç° °ü·ÃµÈ ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.