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°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ½Ã)

TransIT-VirusGEN¢ç Transfection Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 6700
TransIT-VirusGEN¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1.5 §¢
°¡°Ý¹®ÀÇ   transit-virusgen-transfection.pdf
Mirus
MIR 6703
TransIT-VirusGEN¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.3 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6704
TransIT-VirusGEN¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.75 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6705
TransIT-VirusGEN¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 x 1.5 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6706
TransIT-VirusGEN¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 x 1.5 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6845-GMP
TransIT-VirusGEN¢ç GMP Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
150 §¢ (50 L cell culture)
(Package)
°¡°Ý¹®ÀÇ  
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 §¢
°¡°Ý¹®ÀÇ   transduceit-reagent-pds.pdf



*TransIT-VirusGEN¢ç GMP Á¦Ç°ÀÇ °ßÀû ¹× ±¸¸Å´Â ¹Ýµå½Ã ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (T. 02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
*TransIT-VirusGEN »ùÇà ½Åû ½Ã ¡®»ùÇýÅû¼­ (¸µÅ©)¡¯¸¦ ÀÛ¼ºÇϼż­ °í°´Áö¿ø (support@takara.co.kr)·Î º¸³»ÁÖ¼¼¿ä.
Á¦Ç°¼³¸í
TransIT-VirusGEN® Transfection Reagent´Â expression vector¿Í packaging plasmid¸¦ HEK293 ŸÀÔÀÇ ÆÐŰ¡ ¼¼Æ÷·Î È¿À²ÀûÀ¸·Î µµÀÔÇÏ¿©, AAV ¶Ç´Â LentivirusÀÇ »ý»êÀ²À» ³ô¿©ÁÖ´Â transfection ½Ã¾àÀÌ´Ù.
VirusGEN® AAV Transfection Kit¹× VirusGEN® LV Transfection Kit´Â TransIT-VirusGEN® Transfection Reagent¸¦ ±â¹ÝÀ¸·Î plasmid µµÀÔ È¿À²¸¦ ³ôÀ̱â À§ÇÑ enhancer / complex formation ½Ã¾àÀÌ Ãß°¡µÈ kit·Î °¢°¢ AAV¿Í LentivirusÀÇ È¿À²ÀûÀÎ »ý»êÀ» À§ÇØ ÃÖÀûÈ­µÈ Á¦Ç°ÀÌ´Ù.
TransIT-VirusGEN® SELECT Transfection Reagent´Â ¿¬±¸¿ë ½Ã¾àÀΠTransIT-VirusGEN® °ú Á¶¼ºÀº °°Áö¸¸ ÀüÀÓ»ó¿ë ¹ÙÀÌ·¯½º º¤ÅÍ Á¦ÀÛÀ» À§ÇÑ transfection ½Ã¾àÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖµµ·Ï Ç°Áú º¸Áõ ½ÃÇèÀ» ¿Ï·áÇÏ¿´´Ù. ([Example Certificate of Origin (PDF) | Example Certificate of Analysis (PDF) | Example BSE TSE Statement (PDF)])
TransIT-VirusGEN® SELECTÀº PEI, PEIpro® reagentº¸´Ù ¶Ù¾î³­ ¼º´ÉÀ¸·Î, °£´ÜÇÏ°í ÇÕ¸®ÀûÀÎ ºñ¿ëÀ¸·Î ¹ÙÀÌ·¯½º¸¦ ´ë·® »ý»êÇÒ ¼ö ÀÖ´Ù.
TransIT-VirusGEN® GMP Transfection Reagent´Â USP <1043>¿¡ µû¶ó ¼¼Æ÷, À¯ÀüÀÚ ¹× Á¶Á÷ °øÇÐ Á¦Ç° °³¹ß ½Ã º¸Á¶½Ã¾àÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖ¾î, ÀÓ»ó ½ÃÇè ¶Ç´Â »ó¾÷ÀûÀÎ ¹ÙÀÌ·¯½º »ý»ê ½Ã »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
TransduceIT™ Transduction Reagent´Â hexadimethrine bromide ·Î, À̸¦ »ç¿ëÇϸé lentivirus ¶Ç´Â retrovirus transduction È¿À²À» ³ôÀÏ ¼ö ÀÖ´Ù.

±×¸²1. Ÿ»ç´ëºñ ³ôÀº AAV functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A)¿Í Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡ Trans IT-VirusGEN® Transfection Reagent (reagent : DNA (vol : wt) = 2 : 1), Ÿ»ç B4, Ÿ»ç B5, Ÿ»ç C3 (25kDa PEI)¸¦ »ç¿ëÇÏ¿© pAAV-hrGFP, pAAV-RC, pAAV-Helper (1 : 1 : 1 ratio, 1.5 §¶/§¢, Agilent Technologies)¸¦ transfection ÇÏ¿© ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ¿´´Ù. »ý»êµÈ ¹ÙÀÌ·¯½º´Â HT1080¼¼Æ÷¿¡ °¨¿°, 48½Ã°£ µÚ GFP¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.


±×¸²2. Ÿ»ç´ëºñ ³ôÀº Lentivirus functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A), Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡ TransIT-VirusGEN® Transfection Reagent (reagent : DNA (vol : wt) = 3 : 1), Ÿ»ç B4, Ÿ»ç B5, Ÿ»ç C3 (25kDa PEI)¸¦ »ç¿ëÇÏ¿© pLKO.1-puro-CMV-TurboGFP transfer vector, MISSION® Lentivirus Packaging Mix (1 : 1 ratio, 1 §¶/§¢)À» transfection ÇÏ¿´´Ù. TransfectionµÈ ¼¼Æ÷ÀÇ ¹è¾ç »óÃþ¾×À» 0.45 §­ ÇÊÅÍ·Î ¿©°úÇÏ¿© 8 §¶/§¢TransduceIT™ °ú ÇÔ²² 293T/18¿¡ °¨¿°½ÃÄ×´Ù. 72½Ã°£ µÚ GFP¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù


±×¸²3. ´Ù¾çÇÑ AAV »ý»ê ½ºÄÉÀÏ¿¡ Àû¿ë °¡´É
Suspension ÇüÅ·Π¹è¾çÇÑ FreeStyle 293-F ¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A), Adherent ÇüÅ·Π¹è¾çÇÑ 293T / 17 ¼¼Æ÷(B)¿¡ TransIT-VirusGEN® Transfection Reagent¸¦ »ç¿ëÇÏ¿© pAAV -hrGFP, pAAV-RC, pAAV-Helper (1 : 1 : 1 ratio, 1.5 §¶/§¢, Agilent Technologies)¸¦ transfectionÇÏ¿© ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ¿´´Ù (reagent : DNA (vol : wt) = 2 : 1). »ý»êµÈ ¹ÙÀÌ·¯½º´Â HT1080 ¼¼Æ÷¿¡ °¨¿°, 48 ½Ã°£ µÚ¿¡ GFP ¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.
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TransIT-VirusGEN® Transfection Reagent: -10~-30¡É º¸Á¸
(SELECT, GMP ¸ðµÎ µ¿ÀÏ)
°ü·ÃÁ¦Ç°
- ´Ù¾çÇÑ SerotypeÀÇ vector system: AAVpro® Helper Free System (AAV1, 2, 5, 6)
- CRISPR/Cas9 Vector System: AAVpro® CRISPR/Cas9 Helper Free System (AAV2)
- AAV extraction: AAVpro® Extraction Solution
- Cell¿¡¼­ Á¤Á¦: AAVpro® Purification Kit (All Serotypes)
- ¹è¾ç¹èÁö¿Í cell¿¡¼­ ¸ðµÎ Á¤Á¦: AAVpro® Cell & Sup. Purification Kit Maxi (All Serotypes)
- AAV titration: AAVpro® Titration Kit (for Real Time PCR)
- Packaging (293T Cell Line): AAVpro® 293T Cell Line

- Tat-independent Lentivirus system
   : LVpro Packaging Mix (pLVpro-MSCV Vector)
   : LVpro Packaging Mix (pLVpro-EF1α Vector)
   : LVpro Packaging Mix (pLVpro-MSCV-ZsGreen1 Vector)
- °íÈ¿À² °¨¿°À» À§ÇÑ Lentivirus transduction enhancer
   : RetroNectin® (Recombinant Human Fibronectin Fragment)
   : RetroNectin®GMP grade (Recombinant Human Fibronectin Fragment CH-296)

Keyword : AAV,lentifirus,lenti,adeno associated,adeno,virus

È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
>
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
TransIT-VirusGEN¢ç Transfection Reagent
>
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
>
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
>
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
>
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit