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DNA Fragment Purification Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9761A
TaKaRa MiniBEST DNA Fragment Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
68,000¿ø 
85,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9761_e.v1306Da.pdf
Takara
9761B
TaKaRa MiniBEST DNA Fragment Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9761A x 4)
304,000¿ø 


»ó¼¼Á¤º¸
Takara MiniBEST DNA Fragment Purification Kit Ver.4.0Àº PCR°ú ´Ù¸¥ È¿¼Òó¸® ¹ÝÀÀ ÈÄ DNA fragment¸¦ Á¤Á¦Çϱâ À§ÇÑ Á¦Ç°À¸·Î primer(<65 nt)³ª È¿¼Ò, ±×¸®°í nucleotides¸¦ Á¦°ÅÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ¼±ÅÃÀûÀ¸·Î DNA fragment¸¦ ȸ¼öÇϱâ À§ÇÑ ÃÖÀûÈ­µÈ silica membraneÀ¸·Î ±¸¼ºµÈ column°ú Ưº°ÇÑ binding solutionÀÌ Ã¤¿ëÇÏ°í ÀÖ´Ù. Àü °úÁ¤Àº 15ºÐ³» ¿Ï·á °¡´ÉÇÏ´Ù. °¢ columnÀº ÃÖ´ë 20 ug±îÁö DNA fragment(50 bp~20 kbp)¸¦ binding ÇÒ ¼ö ÀÖÀ¸¸ç 70~95% ȸ¼öÀ²À» º¸ÀδÙ. 20 Kbp(20~50 Kbp)ÀÌ»óÀÇ DNA fragmentÀÇ È¸¼öÀ²Àº ÀúÇϵȴÙ. Á¤Á¦µÈ DNA fragment´Â È¿¼Ò, DNA primer³ª dNTP°¡ ¾ø´Â °í¼øµµ DNA·Î ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.

³»¿ë
º» KitÀº Reagent Set¿Í Column SetÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Reagent Set

Buffer DC£ª1

28 ml

Buffer WB£ª2

24 ml

Elution Buffer

2ml x 2


£ª1: Contain strong denaturant. Be careful to avoid contacting with eyes and skin. In the case of such contact, wash immediately with plenty
      water and seek medical advice.
£ª2: Before the first use of the kit, add 56 ml of 100% ethanol to Buffer WB.
Column Set

Spin Columns

50

Collection tubes

50


¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
100% Ethanol, Sterilized water or Tris-HCl(pH8.0)
º¸°ü ¹× ¿î¼Û
1. Store the kit at room temperature (15 - 25¡É).
2. Ship the kit at room temperature (15 - 25¡É).

Keyword : PCR purification

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