Takara¿¡¼¸¸ °¡´ÉÇÑ Æ¯º°ÇÑ NGS kit¸¦ È®ÀÎÇϼ¼¿ä.
Takara¿¡¼´Â ±âÁ¸¿¡ ´Ù¸¥ Á¦Ç°À¸·Î´Â Àû¿ëÀÌ ¾î·Æ°Å³ª È¿À²ÀÌ ¶³¾îÁ³´ø Ưº°ÇÑ »ùÇÿ¡µµ Àû¿ë °¡´ÉÇÑ NGS kit (Illumina ¿ë)¸¦ ÆǸÅÇÏ°í ÀÖ½À´Ï´Ù.
ÇöÀç NGS ÀüÁ¦Ç° 20% ÇÒÀΰú ´õºÒ¾î 200¸¸¿ø ÀÌ»ó ±¸¸Å½Ã »óÇ°±Ç ÁõÁ¤ À̺¥Æ®±îÁö
ÁøÇàµÇ°í ÀÖÀ¸´Ï ¸¹Àº °ü½É ºÎŹµå¸³´Ï´Ù.
¡á
10pg ¶Ç´Â single cell ¼öÁØ ±Ø¼Ò·® mRNA¸¦
full-length ºÐ¼®
- ±Ø¼Ò·®ÀÇ RNA¸¦ 5¡¯¿¡¼ 3¡¯±îÁö full-length
ºÐ¼® (10x NGS µ¥ÀÌÅÍ º¸¿Ï °¡´É)
- º°µµÀÇ RNA ÃßÃâ ¾øÀÌ direct·Î ½ÇÇè
°¡´É
- SMART¢ç (Switching Mechanism at 5 End of RNA Template) ±â¼úÀ» »ç¿ëÇÑ
³ôÀº °¨µµ¿Í ÀçÇö¼º
Code |
Á¦Ç°¸í |
Input ¾ç |
634762 |
SMART-Seq¢ç mRNA LP (with UMIs) |
10pg ~ 100ng |
634786 |
SMART-Seq¢ç mRNA Single Cell LP |
single cell ¼öÁØ |
¡á
³·Àº RIN°ªÀÇ ´Ù¾çÇÑ »ùÇÃ
(FFPE, LCM, cell-free RNA °¡´É)ÀÇ total RNA ºÐ¼®
- ³·Àº qualityÀÇ ±Ø¼Ò·®
»ùÇà ºÐ¼® °¡´É
- Stranded seqÀ¸·Î sense / antisense¸¦ ¼±ÅÃÇÏ¿© ºÐ¼® °¡´É
- Pico / Mid / High inputÀ¸·Î Á¦Ç°¼±Åà °¡´É
Code |
Á¦Ç°¸í |
Input ¾ç |
634354 |
SMART-Seq¢ç Total RNA Pico Input with UMIs
(ZapR¢â Mammalian) |
250 pg ~ 10 ng |
635049 |
SMART-Seq¢ç Total RNA Mid Input |
10 ng ~ 100 ng |
635046 |
SMART-Seq¢ç Total RNA High Input
(RiboGone¢â Mammalian) |
100 ng ~ 1 §¶ |
¡á
Human, Mouse TCR ¹× BCR profiling¿ë Àü¿ë NGS kit
- ÆíÇ⼺ ¾øÀÌ °¡Àå ¸¹Àº clonotypeÀ»
°ËÃâÇÒ ¼ö ÀÖ´Â ¹æ¹ý
- V(D)J ¼¿À» full-length·Î ºÐ¼®Çϰųª
CDR3 ¼¿¸¸ ¼±ÅÃÀûÀ¸·Î ºÐ¼®ÇÏ´Â °Í ¿ª½Ã °¡´É
- Ÿ NGS profiling kit ´ëºñ ³ôÀº °¨µµ·Î ´õ¿í ¸¹Àº µ¥ÀÌÅÍ È®º¸
Code |
Á¦Ç°¸í |
634780 |
SMART-Seq¢ç Human TCR (with UMIs) |
634777 |
SMART-Seq¢ç Human BCR (with UMIs) |
634815 |
SMART-Seq¢ç Mouse TCR (with UMIs) |
634352 |
SMART-Seq¢ç Mouse BCR (with UMIs) |
´Ù¼öÀÇ citationÀ¸·Î Takara Á¦Ç°ÀÇ ³ôÀº ½Å·Úµµ¸¦ È®ÀÎÇϼ¼¿ä~~
[Á¦Ç°
citation À§Á¬]
´ÙÄ«¶óÄÚ¸®¾Æ Á¦Ç°ÆäÀÌÁö ÇÏ´ÜÀ» º¸¸é ½Ç½Ã°£À¸·Î ¾÷µ¥ÀÌÆ®µÇ´Â citation À§Á¬ÀÌ ÀÖ½À´Ï´Ù. Impact Factor / Journals / Authors / Dates º°·Î Á¤·ÄÇÒ ¼ö ÀÖ°í Àüü ¿ø¹®À» È®ÀÎÇÏ´Â °Í ¿ª½Ã °¡´ÉÇÕ´Ï´Ù. Á¦Ç°À» ¼±ÅÃÇϴµ¥ Âü°í ºÎŹµå¸³´Ï´Ù.