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SMARTer¢ç Mouse TCR a/b Profiling Kit (Code 634402, 12ȸ ¿ë·®)°¡ Á¾¸ÅµÇ¾î ¾È³»µå¸³´Ï´Ù.
º» Á¦Ç°Àº Mouse T-cell receptor (TCR) repertoireÀÇ NGS ºÐ¼®À» À§ÇÑ Illumina platform NGS Library Á¦ÀÛ Å°Æ®À̸ç,
SMART-Seq¢ç Mouse TCR (with UMIs) (Code 634815)À¸·Î ¾÷±×·¹À̵åµÇ¾ú½À´Ï´Ù.
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New! SMART-Seq¢ç Mouse TCR (with UMIs) (Code 634815 ¿Ü)
▪ ±âÁ¸ Á¦Ç°°ú °°ÀÌ ³ôÀº °¨µµ·Î TCR ¥á ¿Í TCR ¥â subunit ºÐ¼® °¡´É
▪ Input range°¡ ³Ð¾îÁ® ´Ù¾çÇÑ »ùÇÿ¡ Àû¿ë °¡´É
▪ UMI (unique
molecular identifiers)°¡
Ãß°¡µÇ¾î ºÐ¼® Á¤È®µµ°¡ ³ô¾ÆÁü
▪ Á¦Ç°»ç¿ë À¯¿¬¼ºÀ» À§ÇØ Á¦Ç° ±¸¼º¿¡¼ index primer°¡ Á¦¿ÜµÊ. (Unique Dual Index kits
º°µµ±¸¸Å)
▪ V(D)J full-length sequencing
¶Ç´Â CDR3 sequencing ¿É¼Ç ¼±Åà °¡´É (sequencing ºñ¿ë
Àý°¨ È¿°ú)
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Á¦Ç° |
||
Code |
634402,
634403, 634404 |
634815,
634816, 634814 |
Input |
▪ 10 ng -
500 ng RNA (spleen, thymus, PBMC) ▪ 1,000 -
10,000 purified T cells
|
▪ 1 ng - 1 §¶
RNA (spleen, T cells) ▪ 10 ng - 1
§¶ RNA (whole blood, bone marrow, thymus) ▪ 100 -
10,000 purified T cells
|
UMIs |
¹ÌÀû¿ë |
Àû¿ë |
UDIs |
ȣȯµÇÁö
¾ÊÀ½ |
ȣȯµÊ (º°µµ±¸¸Å) |
Sequencing À¯¿¬¼º
|
2
x 300 bpÀ¸·Î¸¸ Sequencing °¡´É |
Full-length
V(D)J ¶Ç´Â CDR3¸¸ Sequencing °¡´É |
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°ü·ÃµÈ ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô
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