1. °³¿ä
À¯ÀüÀÚ ¹ßÇö Á¤·® PCR ºÐ¼®À» À§ÇÑ Real Time RT-PCR (quantitative PCR; qPCR)¿ë Primer ¼¿ ¹× ÇÕ¼º¼ºñ½º¸¦ Á¦°øÇÕ´Ï´Ù.
´ÙÄ«¶óÀÇ qPCR primer µðÀÚÀÎ ³ëÇÏ¿ì¿Í ÃֽŠÀ¯ÀüÀÚ Á¤º¸ µ¥ÀÌŸº£À̽º¸¦ ÀÌ¿ëÇÑ bioinfomatics¸¦ ¹ÙÅÁÀ¸·Î primer¸¦ µðÀÚÀÎ Ç߱⠶§¹®¿¡, ¹ø°Å·Î¿î µðÀÚÀÎ ÀÛ¾÷À»
»ý·«ÇÒ ¼ö ÀÖ½À´Ï´Ù.
2. ¼ºñ½º ³»¿ë
¹Ì±¹ NCBI database RefSeq (Reference Sequence)¿¡ µî·ÏµÈ human, mouse, rat, dog, bovine, chicken°ú arabidopsis, oryzaÀÇ Å¸°Ù À¯ÀüÀÚ¿¡ ´ëÇØ Æ¯ÀÌÀûÀÎ primer¸¦
°Ë»öÇÒ ¼ö ÀÖ´Â data poolÀÔ´Ï´Ù. À¯ÀüÀÚ¸í µîÀÇ Å°¿öµå, GenBank (RefSeq) accession number, LocusID µîÀ¸·Î ¿Â¶óÀÎ »ó¿¡¼ °Ë»ö ÈÄ, °£´ÜÇÏ°Ô ÁÖ¹® °¡´ÉÇÕ´Ï´Ù.
[»ý¹°Á¾ º° °Ë»ö°¡´ÉÇÑ Å¸°Ù À¯ÀüÀÚ ¼ö]
»ý¹°Á¾ |
Assembly |
Ÿ°Ù À¯ÀüÀÚ¼ö |
Human |
hg38 |
23,517 |
Mouse |
mm10 |
22,428 |
Rat |
rn6 |
16,536 |
Bovine |
bosTau8 |
20,776 |
Dog |
canFam3 |
19,452 |
Chicken |
galGal5 |
17,546 |
Arabidopsis |
TAIR10.37 |
27,231 |
Oryza |
IRGSP-1.0.37 |
29,894 |
3. °¡°Ý ¹× ³³±â
Code |
¼ºñ½º ³»¿ë |
°¡°Ý |
³³±â |
RTC002 |
- Only primer design |
10,000¿ø |
- ¿µ¾÷ÀÏ (10:00~17:00) Á¢¼ö ½Ã: |
RTC001 |
- Primer design + synthesis (PAGE Á¤Á¦) |
100,000¿ø |
- 3ÁÖ: ¼¿Á¤º¸¿Í ÇÔ²² Åùè¹ß¼Û |