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Custom Service


Perfect Real Time Support System

(Real Time PCR Primer µðÀÚÀÎ ¼­ºñ½º)

PRTSS ¼­ºñ½º´Â Å©·Ò(chrome), ¿§Áö(edge) ºê¶ó¿ìÀú¿¡¼­ ÃÖÀûÈ­ µÇ¾î ÀÖ½À´Ï´Ù.
Internet Explorer¿¡¼­´Â Á¤»óÀûÀÎ ÀÛµ¿ÀÌ ¾î·Á¿ï ¼ö ÀÖÀ¸´Ï, ²À Å©·Ò, ¿§Áö ºê¶ó¿ìÀú¸¦ »ç¿ëÇϼ¼¿ä.


  • TB Green¢â intercalator¸¦ ÀÌ¿ëÇÑ Real Time RT-PCR¿¡ ÃÖÀûÀÎ primer °Ë»ö
  • ¿Â¶óÀÎÀ¸·Î °£´ÜÇÏ°Ô °Ë»ö¤ýÁÖ¹® °¡´É
  • TakaraÀÇ TB Green¢âÀ» ÀÌ¿ëÇÑ intercalating ¹ý Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇϸé, ¹ÝÀÀ Á¶°Ç °ËÅä¾øÀÌ ÃÖ»óÀÇ Real Time PCR °á°ú È®ÀÎ °¡´É


¿Â¶óÀÎÁÖ¹®

1. °³¿ä
À¯ÀüÀÚ ¹ßÇö Á¤·® PCR ºÐ¼®À» À§ÇÑ Real Time RT-PCR (quantitative PCR; qPCR)¿ë Primer ¼­¿­ ¹× ÇÕ¼º¼­ºñ½º¸¦ Á¦°øÇÕ´Ï´Ù.
´ÙÄ«¶óÀÇ qPCR primer µðÀÚÀÎ ³ëÇÏ¿ì¿Í ÃֽŠÀ¯ÀüÀÚ Á¤º¸ µ¥ÀÌŸº£À̽º¸¦ ÀÌ¿ëÇÑ bioinfomatics¸¦ ¹ÙÅÁÀ¸·Î primer¸¦ µðÀÚÀÎ Ç߱⠶§¹®¿¡, ¹ø°Å·Î¿î µðÀÚÀÎ ÀÛ¾÷À» »ý·«ÇÒ ¼ö ÀÖ½À´Ï´Ù.


2. ¼­ºñ½º ³»¿ë
¹Ì±¹ NCBI database RefSeq (Reference Sequence)¿¡ µî·ÏµÈ human, mouse, rat, dog, bovine, chicken°ú arabidopsis, oryzaÀÇ Å¸°Ù À¯ÀüÀÚ¿¡ ´ëÇØ Æ¯ÀÌÀûÀÎ primer¸¦ °Ë»öÇÒ ¼ö ÀÖ´Â data poolÀÔ´Ï´Ù. À¯ÀüÀÚ¸í µîÀÇ Å°¿öµå, GenBank (RefSeq) accession number, LocusID µîÀ¸·Î ¿Â¶óÀÎ »ó¿¡¼­ °Ë»ö ÈÄ, °£´ÜÇÏ°Ô ÁÖ¹® °¡´ÉÇÕ´Ï´Ù.

[»ý¹°Á¾ º° °Ë»ö°¡´ÉÇÑ Å¸°Ù À¯ÀüÀÚ ¼ö]

»ý¹°Á¾

Assembly

Ÿ°Ù À¯ÀüÀÚ¼ö

Human

hg38

23,517

Mouse

mm10

22,428

Rat

rn6

16,536

Bovine

bosTau8

20,776

Dog

canFam3

19,452

Chicken

galGal5

17,546

Arabidopsis

TAIR10.37

27,231

Oryza

IRGSP-1.0.37

29,894


3. °¡°Ý ¹× ³³±â

Code

¼­ºñ½º ³»¿ë

°¡°Ý

³³±â

RTC002

- Only primer design
: Primer µðÀÚÀÎ ¼­ºñ½º¸¸ ¹Þ´Â °æ¿ì
: ¼­¿­ Á¤º¸´Â ¸ÞÀÏ·Î ¼ÛºÎ

10,000¿ø

- ¿µ¾÷ÀÏ (10:00~17:00) Á¢¼ö ½Ã:
   ´çÀÏ ¸ÞÀϹ߼Û
- ±× ¿Ü: ÀÍÀÏ ¸ÞÀϹ߼Û

RTC001

- Primer design + synthesis (PAGE Á¤Á¦)
: Primer µðÀÚÀÎ ¹× ÇÕ¼ºÀ» ÇÔ²² ÁøÇàÇÏ´Â °æ¿ì
: Primer 1 set (Forward, Reverse; °¢°¢ 50 pmol/§¡)
: PAGE Á¤Á¦, °¢ 1 OD º¸Áõ

100,000¿ø

- 3ÁÖ: ¼­¿­Á¤º¸¿Í ÇÔ²² Åùè¹ß¼Û

* »ó±â ¼­ºñ½º´Â ´ÙÄ«¶óÄÚ¸®¾Æ ´ë¸®Á¡À» ÅëÇØ °áÁ¦µË´Ï´Ù (°¢ ºÎ°¡¼¼º°µµ).


4. °ü·ÃÁ¦Ç° ¾Æ·¡ÀÇ ´ÙÄ«¶óÁ¦Ç°°ú ÇÔ²² ÀÌ¿ëÇϽøé, º°µµÀÇ ¹ÝÀÀÁ¶°Ç °ËÅä¾øÀÌ ³ôÀº È¿À²·Î Real Time RT-PCRÀ» ÁøÇàÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.

[cDNA ÇÕ¼º ½Ã¾à]

[Real Time PCR ½Ã¾à- Intercalator¹ý]

[Âü°í]
One Step TB Green¢â PLUS PrimeScript RT-PCR Kit (Perfect Real Time) (Code RR096A/B)£ªµî 1 step Real Time RT-PCR½Ã¾àÀ» »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. ÇÏÁö¸¸, primer¿¡ µû¶ó¼­´Â ÃæºÐÇÑ °á°ú¸¦ ¾òÁö ¸øÇÏ´Â °æ¿ì°¡ ÀÖÀ¸¸ç ±× °æ¿ì 2 step Real Time RT-PCRÀ» ±ÇÀåÇÕ´Ï´Ù.

¡Ø º» ¼­ºñ½º·Î ¼³°èµÇ´Â Real Time PCR¿ë primer´Â ½ÇÇè ¼º°øÀ» 100% º¸ÁõÇÏ´Â °ÍÀº ¾Æ´Ï¹Ç·Î À¯ÀÇÇϽñ⠹ٶø´Ï´Ù.