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Standard qPCR Mix

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
RR820A
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º  »ùÇýÅû 
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR820A_e.v2112Da.pdf
Takara
RR820B
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
400 ȸ (RR820A x 2)
572,000¿ø 
Takara
RR820L
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus), Bulk
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR820L_e.v2112Da.pdf
Takara
RR820LB
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus), Bulk
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
400 ȸ (RR820LA x 2)
572,000¿ø 
Takara
RR82LR
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus), ROX Plus
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR82LR_e.v2112Da.pdf
Takara
RR82LRB
TB Green¢ç Premix Ex Taq¢â II (Tli RNaseH Plus), ROX Plus
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
400 ȸ (RR82LRA x 2)
572,000¿ø 



  • Real Time PCRÀ» ÀÌ¿ëÇÏ¿© À¯ÀüÀÚÀÇ °ËÃâ ¹× Á¤·®À» ºü¸£°í Á¤È®ÇÏ°Ô ¼öÇà.
  • TB Green¢çÀÌ ¹Ì¸® È¥ÇÕµÈ 2¡¿ premix ½Ã¾àÀ¸·Î primer ¹× template¿Í ¸ê±Õ Áõ·ù¼ö¸¦ ÷°¡ÇÏ´Â °Í¸¸À¸·Î intercalating ¹ý¿¡ ÀÇÇÑ real time PCRÀ» ¼öÇà.
  • Hot Start PCR ¿ë È¿¼ÒTAKARA Ex Taq HS¿Í Real Time PCR¿ëÀ¸·Î ÃÖÀûÈ­µÈ buffer¸¦ »ç¿ëÇϱ⠶§¹®¿¡ ÁõÆø È¿À²ÀÌ ÁÁ°í, °í°¨µµÀÇ °ËÃâÀÌ °¡´É.
  • 2¡¿ premix¿¡ ³»¿­¼º RNaseHÀÎ Tli RNaseH°¡ ¹Ì¸® ÷°¡µÇ¾î ÀÖ¾î cDNA¸¦ ÁÖÇüÀ¸·Î ÇÑ °æ¿ì, ÀÜÁ¸ÇÏ´Â mRNA¿¡ ÀÇÇÑ PCR ÀúÇØ ¹ÝÀÀÀ» ±ØÇѱîÁö ¾ïÁ¦ÇÑ´Ù.
  • º» Á¦Ç°Àº ÀϹÝÀûÀÎ Real Time PCR Ÿ°Ù (»çÀÌÁî 80-150 bp)ÀÇ ÁõÆø¿¡ ÃÖÀûÈ­µÇ¾î ÀÖ´Â ½Ã¾àÀÔ´Ï´Ù.
    Á» ´õ ±ä »çÀÌÁîÀÇ ÁõÆøÀ» ¿øÇÏ´Â °æ¿ì, ¾Æ·¡ÀÇ Á¦Ç°À» Ãßõµå¸³´Ï´Ù.
    - TB Green¢ç Fast qPCR Mix (Code RR430A/B): Á¦Ç° ¹Ù·Î°¡±â
    - TB Green¢ç Premix Ex Taq¢â (Tli RNaseH Plus) (Code RR420A/B): Á¦Ç° ¹Ù·Î°¡±â
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TB Green¢ç Premix Ex Taq (Tli RNaseH Plus)Àº, TB Green¢çÀ» ÀÌ¿ëÇÑ intercalating ¹ý Real Time PCR Àü¿ë ½Ã¾àÀÌ´Ù. 2¡¿ ³óµµÀÇ premix typeÀÇ ½Ã¾àÀ¸·Î ½Ç½Ã°£ ¸ð´ÏÅ͸µ¿¡ ÀûÇÕÇÑ ³óµµÀÇ TB Green¢ç¸¦ ¹Ì¸® Æ÷ÇÔÇÏ°í ÀÖ¾î ¹ÝÀÀ ¾×ÀÇ Á¶Á¦°¡ °£´ÜÇÏ´Ù.
¶ÇÇÑ, ³»¿­¼º RNaseHÀÎ Tli RNaseH °¡ 2¡¿ Premix ½Ã¾à Áß¿¡ ÷°¡µÇ¾î ÀÖ¾î cDNA¸¦ ÁÖÇüÀ¸·Î ÇÑ °æ¿ì ÀÜÁ¸ mRN¿¡ ÀÇÇÑ PCR ¹ÝÀÀ ÀúÇظ¦ ±ØÇÑÀ¸·Î ¾ïÁ¦ ÇÒ ¼ö ÀÖ´Ù.



º» Á¦Ç°Àº buffer Á¶¼ºÀ» º¯ÇüÇÏ¿© TB Green¢ç Premix Ex Taq (Tli RNaseH Plus) (Á¦Ç° ÄÚµå RR420A)¿¡ ºñÇØ ¹ÝÀÀ ƯÀ̼ºÀ» ³ô¿© Á¤·®À» ¹æÇØÇÏ´Â ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÏ¿©, Æø³ÐÀº ¹üÀ§¿¡¼­ º¸´Ù Á¤È®ÇÑ Á¤·®ÀÌ °¡´ÉÇÏ´Ù. ÀÌ buffer¿Í anti-TaqÇ×ü¸¦ ÀÌ¿ëÇÑ Hot Start PCR ¿ë È¿¼Ò TAKARA Ex Taq HS¿Í Á¶ÇÕÇÏ¿©, ÀçÇö¼º ¹× ½Å·Úµµ ³ôÀº real time PCR Çؼ®ÀÌ °¡´ÉÇÏ´Ù.

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ÁÖ) Smart Cycler System / Smart Cycler II System (Cepheid)´ÂTB Green¢ç Premix Ex Taq (Tli RNaseH Plus) (Á¦Ç° ÄÚµå RR420A)ÀÇ »ç¿ëÀ» ±ÇÀåÇÕ´Ï´Ù.

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TB Green¢ç Premix Ex Taq II (Tli RNaseH Plus) (Code RR820A) (200 ȸ, 50 ¥ìl ¹ÝÀÀ½Ã)

TB Green¢ç Premix Ex Taq II (Tli RNaseH Plus (2¡¿conc.)*1

1 ml¡¿5

ROX Reference Dye (50¡¿conc.)*2

200 ¥ìl

ROX Reference Dye II (50¡¿conc.)*2

200 ¥ìl


TB Green¢ç Premix Ex Taq II (Tli RNaseH Plus), Bulk (Code RR820L) (200 ȸ, 50 ¥ìl ¹ÝÀÀ½Ã)

TB Green¢ç Premix Ex Taq (Tli RNaseH Plus), Bulk*1

5 ml

ROX Reference Dye (50¡¿conc.)*3

200 ¥ìl


TB Green¢ç Premix Ex Taq II (Tli RNaseH Plus), ROX plus (Code RR82LR) (200 ȸ, 50 ¥ìl ¹ÝÀÀ½Ã)

TB Green¢ç Premix Ex Taq (Tli RNaseH Plus), ROX plus*4

5 ml


*1 TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, Tli RNaseHÆ÷ÇÔ

*2 Life TechnologiesÀÇ Real Time PCR ÀåÄ¡ µî well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÇÏ´Â ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
    ABI PRISM 7000/7700, Applied Biosystems 7900HT/7300 Real - Time PCR System°ú StepOnePlus¿¡´Â ROX Reference Dye¸¦,
    7500 Real - Time PCR System ¹× 7500 Fast Real - Time PCR System¿¡´Â ROX Reference Dye II°¡ ÀûÇÕÇÏ´Ù.
    Agilent Mx3000PÀÇ °æ¿ì¿¡´Â ROX Reference Dye II°¡ ÀûÇÕÇÏ´Ù.
    CronoSTAR¢â 96 Real-Time PCR System (4ch), TaKaRa Thermal Cycler Dice Real Time System °ú Smart Cycler System, LightCycler¿¡¼­´Â ÇÊ¿äÇÏÁö ¾Ê´Ù.

*3 ROX Reference Dye(50¡¿)·Î ABI PRISM 7000/7700, Applied Biosystems 7900HT/7300 Real - Time PCR System°ú StepOnePlus
    »ç¿ë½Ã¿¡´Â 1/50·Î »ç¿ë, 7500 Real - Time PCR System ¹× 7500 Fast Real - Time PCR System »ç¿ë½Ã¿¡´Â 1/250·Î »ç¿ëÇÑ´Ù.

*4 TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, Tli RNaseH ¹× ROX Reference Dye¸¦ Æ÷ÇÔÇÑ´Ù.
    ROX Reference Dye II ·Î º¸Á¤ÇØ¾ß ÇÏ´Â Applied Biosystems 7500/7500 Fast Real-Time PCR System(Life Technologies)¿¡´Â
    ROX Reference Dye°¡ º°µµ·Î ÷ºÎµÇ¾î ÀÖ´Â TB Green¢ç Premix Ex Taq II (Tli RNaseH Plus), Bulk (Code RR820L)ÀÇ »ç¿ëÀ» ÃßõÇÑ´Ù.
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4 ¡É, º¸Á¸ : 6 °³¿ù°£ ¾ÈÁ¤
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- Àå±â°£ ÀúÀåÇÏ·Á¸é - 20 ¡É º¸°üÇϽʽÿÀ
- ¿ëÇØ ÈÄ¿¡´Â 4 ¡É º¸°üÇÏ°í, 6°³¿ù ¾È¿¡ »ç¿ë ÇÒ °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
(°¢ Á¦Ç°ÀÇ ¸Å´º¾ó V. StorageÀÇ Ãßõ¿Âµµ ÂüÁ¶)
ÇÔ²² »ç¿ëÇÏ´Â Á¦Ç°
<cDNA ÇÕ¼ººÎÅÍ TB Green¢ç premix±îÁö ÇÔ²² »ç¿ëÇϸé Perfect Real Time PCR>

<Reference paper list>
³í¹®Á¦¸ñ

³í¹®¸í

»ç¿ëÁ¦Ç°

Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture

Front Plant Sci. 2016; 7: 1302.

RR047,
RR820

Identification of Putative ORF5 Protein of Porcine Circovirus Type 2 and Functional Analysis of GFP-Fused ORF5 Protein

PLoS One. 2015; 10(6): e0127859.

RR047,
RR820

Glucagon-like peptide-1 protects cardiomyocytes from advanced oxidation protein product-induced apoptosis via the PI3K/Akt/Bad signaling pathway

Mol Med Rep. 2016 Feb; 13(2): 1593?1601.

RR047,
RR820

Genome-wide identification of vegetative phase transition-associated microRNAs and target predictions using degradome sequencing in Malus hupehensis

BMC Genomics. 2014; 15(1): 1125.

RR047,
RR820

Antennal transcriptome analysis of the Asian longhorned beetle Anoplophora glabripennis

Sci Rep. 2016; 6: 26652.

RR047,
RR820

Identification of heat-responsive genes in carnation (Dianthus caryophyllus L.) by RNA-seq

Front Plant Sci. 2015; 6: 519.

RR047,
RR820

Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

Biomed Res Int. 2015; 2015: 768478.

RR047,
RR820


Keyword : Real Time PCR,qPCR,sybr green

Intercalator TB Green °ËÃâ
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Standard qPCR Mix
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Long target¿ë qPCR Mix
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Fast qPCR Mix
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Primer dimer, ºñƯÀÌÀûÀÎ ÁõÆøÀ» °³¼±Çϱâ À§..
Dimer eraser qPCR Mix
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genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
GC rich qPCR Mix
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Crude sample¿¡¼­ Real-Time PCR ºÐ¼®
Crude »ùÇÿë qPCR Mix
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»ç¿ëÀÌ Æí¸®ÇÑ 1 step RT-qPCR Kit
Premixed 1 step qRT-PCR
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Specific 1 step qRT-PCR
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TB Green¢çÀ» »ç¿ëÇÑ 1 step real-time RT-PCR
Standard 1 step qRT-PCR
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Real Time PCR¿ë ¿ÜºÎ Ç¥ÁØRNA£¦ÇÁ¶óÀ̸Ó
External Standard Kit (¥ëpolyA) for qPCR
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Transgenic mouseÀÇ screening ¿ë primer set
Transgene Detection Primer Set for Real Time (Mouse)
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[New] Fast, UNG, dye plus Premix