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Bacteria (tuf gene) Quantitative PCR Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR240A
Bacteria (tuf gene) Quantitative PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
(Package)
622,400¿ø 
778,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR240A_e.v2203Da.pdf

Á¦Ç°¼³¸í
½Ã·á³» ¹Ì»ý¹° ¼ö¸¦ Real Time PCR·Î Á¤·®

Bacteria (tuf gene) Quantitative PCR KitÀº ½ÄÇ°À̳ª ȯ°æ ºÐ¾ßÀÇ ¼¼±ÕÀÇ ¼ö¸¦ Real Time PCR·Î Á¤·®ÇÏ´Â Á¦Ç°ÀÌ´Ù. ±âÁ¸ÀÇ ºÐ¼®¹æ¹ýÀÌ 2~4ÀÏÁ¤µµ °É¸®´Â ¹è¾çÀÌ ÇÊ¿äÇÏ°í ¹è¾çÁ¶°ÇÀÇ ÃÖÀûÈ­°¡ ÇÊ¿äÇß´ø °Í¿¡ ºñÇØ ¹Î°¨ÇÏ°í ½Å¼ÓÇÏ°Ô °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº bacterial protein elongation factor Tu (tuf ) gene¸¦ ÁõÆøÇؼ­ TB Green¢â °ËÃâ¹ýÀ» ÀÌ¿ëÇÏ¿© ½Ç½Ã°£À¸·Î ÃøÁ¤ÇÑ´Ù. Tuf À¯ÀüÀÚ´Â ¹Ì»ý¹° Á¾°£¿¡ º¸Á¸ÀÌ ÀßµÈ À¯ÀüÀÚ·Î Çϳª ¶Ç´Â µÎ °³*ÀÇ chromosome Ä«ÇǸ¦ °®°í ÀÖ´Ù. º» Á¦Ç°À¸·Î °ËÃâµÇ´Â ¹Ì»ý¹° Á¾Àº ¸Å´º¾óÀÇ Table 3¿¡ Ç¥±âµÇ¾îÀÖ°í Á¦Ç°³» primer¼­¿­ (GenBank, current as of November 21, 2011)°ú 100% ÀÏÄ¡ÇÏ´Â ¹Ì»ý¹° °Ô³ð ¼­¿­Àº º°µµ ÆÄÀÏÀ» Âü°í ¹Ù¶õ´Ù.

* Tanaka Y et al. (2010) Design of a new universal real-time PCR system targeting the tuf gene for the enumeration of bacterial counts in food. J. Food Prot. 73: 670-679.
³»¿ë

(25¥ì l¹ÝÀÀ, 100ȸºÐ)

TB Green¢â Premix Ex Taq GC*1

2¡¿conc.

625 ¥ìl¡¿2

TUF Primer Mix

5¡¿conc.

250 ¥ìl¡¿2

dH2O

1 ml

ROX Reference Dye*2

50¡¿conc.

50 ¥ìl

ROX Reference Dye II*2

50¡¿conc.

50 ¥ìl

TUF Positive Control

1¡¿105 copies/¥ìl

100 ¥ìl

EASY Dilution (for Real Time PCR)

1ml¡¿2


*1 TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, and TB Green¢â°¡ Æ÷ÇԵǾî ÀÖ´Ù.
*2 ROX Reference Dye´Â StepOnePlus Real-Time PCR Systems¿¡ »ç¿ëÇÏ°í ROX Reference Dye II´Â 7500 Real-Time PCR Systems¿¡ »ç¿ëÇÑ´Ù. Thermal Cycler Dice¿¡´Â ROX dye°¡
   ÇÊ¿äÇÏÁö ¾Ê´Ù.
¿î¼Û
- 20¡É/ -80¡É
ÀúÀå
- TB Green¢â Premix Ex Taq GC(Package1)
* À¶ÇØ ÈÄ 4¡É Â÷±¤ º¸Á¸½Ã 6°³¿ù ¾ÈÁ¤(Àå±â º¸Á¸Àº -80¡É)
* Àå±â º¸Á¸½Ã¿¡´Â -80¡É¿¡ ÀúÀåÇϽʽÿÀ.(-20¡É º¸Á¸Àº ÇÇÇϽʽÿÀ.)

- ´Ù¸¥ ±¸¼º ¿ä¼Ò(Package2): -20¡É ÀúÀå

Keyword :

º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (Real Time PCR)
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