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TB Green¢çÀ» »ç¿ëÇÑ 1 step real-time RT-PCR

Standard 1 step qRT-PCR

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR066A
One Step TB Green¢ç PrimeScript¢â RT-PCR Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
(50 §¡ ¹ÝÀÀ°è)
335,200¿ø 
419,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR066A_e.v2202Da.pdf
Takara
RR066B
One Step TB Green¢ç PrimeScript¢â RT-PCR Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ȸ (RR066A x 5)
1,890,000¿ø 

  • 1 step real-time PCR·Î ÃÖÀûÈ­µÈ buffer
  • ³ôÀº ÁõÆøÈ¿À²ÀÇ °í°¨µµ ½Ã¾à
Á¦Ç°¼³¸í
One Step TB Green® PrimeScript™ RT-PCR Kit (Perfect Real Time)Àº intercalator·Î TB Green®À» »ç¿ëÇÑ 1 step RT-qPCR KitÀÌ´Ù. Single tube¿¡¼­ ¿ªÀü»ç ¹ÝÀÀ (Reverse transcription)ºÎÅÍ qPCR±îÁö ¿¬¼ÓÀûÀ¸·Î ¹ÝÀÀÀÌ ÀÌ·ç¾îÁö±â¿¡ Á¶ÀÛÀÌ °£ÆíÇÏ°í ¿À¿° À§ÇèÀÌ ³·´Ù.
PCRÀÇ ÁõÆø »ê¹°À» ½Ç½Ã°£À¸·Î °ËÃâÇϱ⠶§¹®¿¡ Àü±â¿µµ¿ µîÀ¸·Î º°µµ·Î È®ÀÎÇÒ ÇÊ¿ä°¡ ¾ø°í RNA ¹ÙÀÌ·¯½º µîÀÇ ¹Ì·® »ùÇÃÀÇ °ËÃâ¿¡ À¯¸®ÇÏ´Ù.
ÀÌ Á¦Ç°Àº ´ÙÄ«¶óÀÇ °í¼º´É High fidelity RTase, PrimeScript RTase¿Í ÁõÆø È¿À²ÀÌ ¶Ù¾î³­ °í¼º´É PCR È¿¼Ò TaKaRa Ex Taq® HS¸¦ »ç¿ëÇØ 1 step RT-PCR ¿ëÀ¸·Î ÃÖÀûÈ­µÇ¾î Àú³óµµÀÇ RNA »ùÇÿ¡¼­µµ °í°¨µµ °ËÃâÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ³ôÀº ¹ÝÀÀ ƯÀ̼ºÀ¸·Î Á¤È®ÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
Àû¿ë ±âÁ¾
ÀÌ Á¦Ç°Àº ¾Æ·¡ÀÇ °¢Á¾ PCR±â±â¿ÍÀÇ È£È¯¼ºÀÌ È®ÀεǾú´Ù.
 - Thermal Cycler Dice Real Time System III (Code TP950 ¿Ü)
 - CronoSTAR 96 Real-Time PCR System (Code 640231 ¿Ü)
 - Appied Biosystems 7300/7500/7500 Fast Real-Time PCR System
 - StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)
 - LightCycler (Roche Diagnostics»ç)
 - Smart Cycler System/Smart Cycler II System (Cepheid»ç)µî
ÀÌ¿Ü¿¡µµ FAM (e.g. TB Green®) ä³ÎÀ» °¡Áø ÀϹÝÀûÀÎ qPCR ±â±â¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50 §¡ ¹ÝÀÀ¾×, 100ȸºÐ)

2×One Step TB Green® RT-PCR Buffer III*1

3x 840 §¡

TaKaRa Ex Taq HS (5 U/§¡)

100 §¡

PrimeScript RT enzyme Mix II*2

100 §¡

RNase Free dH2O

2x 1.25 §¢

ROX Reference Dye (50×conc)*3

100 §¡

ROX Reference Dye II (50×conc)*3

100 §¡

*1 dNTP Mixture, Mg2+ ¹× TB Green®À» Æ÷ÇԵǾî ÀÖ´Ù.
*2 RNase inhibitor¸¦ Æ÷ÇԵǾî ÀÖ´Ù.
*3 Applied BiosystemÀÇ qPCR ±â±â µî¿¡¼­ well°£ÀÇ ½ÅÈ£¸¦ º¸Á¤Çϴµ¥ »ç¿ëÇÑ´Ù.
ÀÚ¼¼ÇÑ ROX reference dye »ç¿ë¿¡ ´ëÇÑ ³»¿ëÀº °¢ ±â±âÀÇ Á¦Á¶»ç¿¡ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
º¸Á¸ -20¡É
¡Ø 2×One Step TB Green® RT-PCR Buffer III´Â Â÷±¤ÇÏ¿© -20¡É¿¡ º¸°üÇÑ´Ù
»ç¿ë»óÀÇ ÁÖÀÇ
º» Á¦Ç° ÀÌ¿ë½Ã ¿ªÀü»ç ¹ÝÀÀÀº PCR¿ë specific primer¸¦ »ç¿ëÇÏ°í, Random Primer, Oligo dT Primer´Â »ç¿ëÇÒ ¼ö ¾ø´Ù.
°ü·ÃÁ¦Ç°
- gDNA Á¦°Å columnÀÌ Æ÷ÇÔµÈ RNA ÃßÃâ Kit, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
- Virus À¯·¡ RNA ȤÀº DNA Á¤Á¦ Kit, TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Code 9766A)
- AGPC¹ýÀ» È°¿ëÇÑ RNA Á¤Á¦ Kit, RNAiso Plus (Code 9109 ¿Ü)

Keyword :

Intercalator TB Green °ËÃâ
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Standard qPCR Mix
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Long target¿ë qPCR Mix
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Fast qPCR Mix
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Primer dimer, ºñƯÀÌÀûÀÎ ÁõÆøÀ» °³¼±Çϱâ À§..
Dimer eraser qPCR Mix
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genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
GC rich qPCR Mix
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Crude sample¿¡¼­ Real-Time PCR ºÐ¼®
Crude »ùÇÿë qPCR Mix
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»ç¿ëÀÌ Æí¸®ÇÑ 1 step RT-qPCR Kit
Premixed 1 step qRT-PCR
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ºñƯÀÌÀûÀÎ ÁõÆøÀ» È¿°úÀûÀ¸·Î ¾ïÁ¦ÇÏ´Â 1 step..
Specific 1 step qRT-PCR
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TB Green¢çÀ» »ç¿ëÇÑ 1 step real-time RT-PCR
Standard 1 step qRT-PCR
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Real Time PCR¿ë ¿ÜºÎ Ç¥ÁØRNA£¦ÇÁ¶óÀ̸Ó
External Standard Kit (¥ëpolyA) for qPCR
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Transgenic mouseÀÇ screening ¿ë primer set
Transgene Detection Primer Set for Real Time (Mouse)
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¶Ù¾î³­ ¼º´ÉÀÌ °¡µæ- ³ôÀº ƯÀ̼º, °í¼Ó¹ÝÀÀ, U..
[New] Fast, UNG, dye plus Premix