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qPCR¿ë cDNAÇÕ¼º All-in-One kit

RT components (qPCR¿ë)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR037A
PrimeScript¢â RT reagent Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(10 §¡ ¹ÝÀÀ°è)
295,200¿ø 
369,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR037A_e.v2202Da.pdf
Takara
RR037B
PrimeScript¢â RT reagent Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (RR037A x 4)
1,328,000¿ø 
Takara
9160
EASY Dilution (for Real Time PCR)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml ¡¿ 8
183,200¿ø 
229,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9160_DS.v1902Da-a_116885.pdf



  • ºÒ°ú 15ºÐÀÇ ¹ÝÀÀÀ¸·Î ¿ªÀü»ç¹ÝÀÀ ¿Ï·á
  • ¿ªÀü»ç ¹ÝÀÀ¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ kit ³»¿¡ Æ÷ÇÔ
  • 2Á¾·ùÀÇ primer Áß ¿øÇÏ´Â primer¸¦ ¼±ÅÃÇؼ­ Real Time PCR¿¡ ÃÖÀûÀÎ ÁÖÇü cDNA¸¦ ÇÕ¼º
Á¦Ç° ¼³¸í
PrimeScript¢â RT reagent Kit (Perfect Real Time)Àº 2 step Real Time RT-PCR ¿¡ ÃÖÀûÈ­µÈ ¿ªÀü»ç ¹ÝÀÀ¿ë kitÀÌ´Ù.
½ÅÀ强ÀÌ ¶Ù¾î³­ PrimeScript¢â RTase¸¦ »ç¿ëÇÏ¿© ´Ü½Ã°£ÀÇ ¹ÝÀÀÀ¸·Î È¿À²ÀûÀÎ Real time PCR¿ëÀÇ ÁÖÇü cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ°í, ½ÇÇèÁ¶ÀÛÀÌ °£´ÜÇÏ¿© high throughput Çؼ®¿¡µµ ÀûÇÕÇÏ´Ù.
Á¦Ç°¿¡´Â ¿ªÀü»ç¿ë ÇÁ¶óÀ̸ӷΠRandom 6 mers¿Í Oligo dT ÇÁ¶óÀ̸Ӱ¡ Æ÷ÇԵǾî ÀÖ¾î ½ÇÇè¸ñÀû¿¡ µû¶ó¼­ µÎ Á¾·ùÀÇ ÇÁ¶óÀ̸Ӹ¦ È¥ÇÕ ¶Ç´Â ´Üµ¶À¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ Æ¯Á¤À¯ÀüÀÚÀÇ °ËÃâ¿¡´Â gene specific ÇÁ¶óÀ̸ӵµ »ç¿ë °¡´ÉÇÏ´Ù.
º» Á¦Ç°¿¡ ÀÇÇØ ÇÕ¼ºµÈ cDNA´Â TB Green¢â assay, Dual-Labeled Probe assay¿¡ ¸ðµÎ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ¸ñÀû¿¡ µû¶ó TB Green¢â Premix Ex Taq¢â II (Tli RNaseH Plus) (Code RR820A), TB Green¢â Fast qPCR Mix (Code RR430A), Probe qPCR Mix (Code RR391A) µî Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇÑ´Ù.
³»¿ë (200 ȸ, 10 §¡ ¹ÝÀÀ½Ã)

1.

5 X PrimeScript Buffer (for Real Time)*1

400 §¡

2.

PrimeScript RT Enzyme Mix I*2

100 §¡

3.

Oligo dT Primer (50 ¥ìM)

100 §¡

4.

Random 6mers (100 ¥ìM)

400 §¡

5.

RNase Free dH2O

1§¢

6.

EASY Dilution (for Real Time PCR)*3

1§¢


*1 dNTP Mixture ¹× Mg2+À» Æ÷ÇÔ.
*2 RNase Inhibitor¸¦ Æ÷ÇÔ.
*3 total RNA³ª cDNA¸¦ ´Ü°è Èñ¼®ÇÒ ¶§ Èñ¼®¿ë¾×À¸·Î »ç¿ëÇÑ´Ù. H2O³ª TE buffer·Î Èñ¼®Çϸé Á¤È®ÇÑ Èñ¼®À» ÇÒ ¼ö ¾ø´Â °æ¿ì°¡ ÀÖÁö¸¸, EASY DilutionÀ» ÀÌ¿ëÇϸé Àú³óµµ±îÁö Á¤È®È÷ Èñ¼®À» ÇÒ ¼ö ÀÖ´Ù. º» buffer´Â ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCRÀÇ ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÀ¸¹Ç·Î Èñ¼®ÇÑ ÁÖÇü ¿ë¾×À» ±×´ë·Î ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCR ¹ÝÀÀÀÇ ÁÖÇüÀ¸·Î¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
EASY Dilution (for Real Time PCR) (Code 9160)´Â º°µµ ±¸¸Å °¡´É.

¡¼ÁÖÀÇ¡½EASY DilutionÀº TakaraÀÇ Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇϱ⸦ ±ÇÀåÇϸç, Ÿ»ç Á¦Ç°°úÀÇ È£È¯¼ºÀº È®ÀεÇÁö ¾Ê¾ÒÀ½.
º¸Á¸ -20 ¡É

Keyword : Real Time PCR,qPCR,sybr green,RT kit,cDNAÇÕ¼º

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Universal dilution solution
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