´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > 2 x all premix 1 step qRT-PCR kit
½Å¼ÓÇÑ À¯ÀüÀÚ¹ßÇö Çؼ®À» À§ÇÑ 1 step qRT-PCR premix

2 x all premix 1 step qRT-PCR kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR600A
One Step PrimeScript¢â III RT-qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200ȸ
390,400¿ø 
488,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR600A_e.v2111Da.pdf
Takara
RR600B
One Step PrimeScript¢â III RT-qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1,000ȸ (RR600A x 5)
2,196,000¿ø 
Takara
RR601A
One Step PrimeScript¢â III RT-qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200ȸ
397,600¿ø 
497,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR601A_e.v2111Da.pdf
Takara
RR601B
One Step PrimeScript¢â III RT-qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1,000ȸ (RR601A x 5)
2,235,000¿ø 


  • 2 x premix ŸÀÔÀÇ 1 step qRT-PCR kit (Dual-Labeled Probe °ËÃâ¹ý)
  • Ź¿ùÇÑ ¹ÝÀÀ¼ºÀ¸·Î ¹ÝÀÀ ¾ïÁ¦¹°ÁúÀ» Æ÷ÇÔÇÑ »ùÇÃ, crude »ùÇà ÁõÆø¿¡µµ ÃÖÀû
  • Carryover contamination ¹æÁö¸¦ À§ÇÑ UNG plus ¹öÀü ¼±Åà °¡´É

º» Á¦Ç°ÀÇ ´ë¿ë·® Æ÷Àå ¶Ç´Â °í³óµµ (5X, GPR) Á¦Ç° ±¸¸Å¸¦ ¿øÇÏ´Â °æ¿ì ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Dual Labeled Probe¹ýÀÇ 1 step Real Time RT-PCR Àü¿ë premix ½Ã¾àÀÌ´Ù.
º» Á¦Ç°Àº qPCRÀ» ¼öÇàÇϱâ À§ÇÑ ¿ªÀü»çÈ¿¼Ò¿Í PCR È¿¼Ò ¹× ¹ÝÀÀ buffer°¡ premixÈ­ µÇ¾î ÀÖ¾î ÇϳªÀÇ Æ©ºê ³»¿¡¼­ Çѹø¿¡ RT-PCR ¹ÝÀÀÀ» ÁøÇàÇÒ ¼ö ÀÖ¾î, Á¶ÀÛÀÌ °£ÆíÇÏ°í contaminationÀÇ À§ÇèÀ» ³·Ãâ ¼ö ÀÖ´Ù.
¿ªÀü»ç È¿¼Ò´Â ³»¿­¼ºÀÌ ³ô°í, ¹ÝÀÀ ¾ïÁ¦¹°ÁúÀÌ Á¸ÀçÇÏ¿©µµ È¿À²ÀûÀ¸·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÑ PrimeScript RTase III ¸¦ »ç¿ëÇÏ°í ÀÖ´Ù.
¶ÇÇÑ º» Á¦Ç°Àº º¹¼öÀÇ ¹ÝÀÀÀ» µ¿½Ã¿¡ ½Ç½ÃÇÏ´Â multiplex Real Time RT-PCR ¹ÝÀÀ¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
¹Ýº¹ÀûÀÎ PCR ¹ÝÀÀ¿¡¼­ ¹ß»ý À§ÇèÀÌ ³ôÀº carryover contaminationÀ» ¹æÁöÇϱâ À§ÇØ UNG¸¦ Æ÷ÇÔÇÑ One Step PrimeScript¢â III RT-qPCR Mix, with UNG (Code RR601S/A/B)µµ ÆǸÅÇÏ°í ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé RNA/DNA virusÀÇ Á¶±â °ËÃâÀ̳ª mRNA ¹ßÇöÇؼ®À» Á»´õ °£ÆíÇÏ°í Á¤È®ÇÏ°Ô ÁøÇàÇÒ ¼ö ÀÖ´Ù.

º» Á¦Ç°Àº Thermal Cycler Dice¢ç Real Time System ÀåÄ¡¿¡ ÃÖÀûÈ­µÇ¾î ÀÖÁö¸¸, ÁÖ¿ä Real Time PCR ÀåÄ¡¿¡µµ »ç¿ëÇÒ ¼ö ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.
ÁÖÀÇ: Intercalater (TB Green¢â)¹ýÀ» ÀÌ¿ëÇÑ 1 step Real Time RT-PCR¿¡´Â ¾Æ·¡ÀÇ Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
³»¿ë
One Step PrimeScript¢â III RT-qPCR Mix (Code RR600A, 200ȸ; 25§¡ ¹ÝÀÀ ½Ã)

±¸¼ºÇ°¸í

¿ë·®

1.

One Step PrimeScript¢â III RT-qPCR Mix (2¡¿)

625 §¡ ¡¿ 4

2.

RNase Free Water

1.25 §¢ ¡¿ 2

3.

ROX Reference Dye (50¡¿conc.)*

100 §¡

4.

ROX Reference Dye II (50¡¿conc.)*

100 §¡


One Step PrimeScript III RT-qPCR Mix, with UNG (Code RR601A, 200ȸ; 25§¡ ¹ÝÀÀ ½Ã)

±¸¼ºÇ°¸í

¿ë·®

1.

One Step PrimeScript¢â III RT-qPCR Mix, with UNG (2¡¿)

625 §¡ ¡¿ 4

2.

RNase Free Water

1.25 §¢ ¡¿ 2

3.

ROX Reference Dye (50¡¿conc.)*

100 §¡

4.

ROX Reference Dye II (50¡¿conc.)*

100 §¡


* Life TechnologiesÀÇ Real Time PCR ÀåÄ¡¿Í °°ÀÌ well °£ Çü±¤ ½ÅÈ£ º¸Á¤ÀÌ ÇÊ¿äÇÑ ÀåÄ¡¿¡ »ç¿ëÇϸç, ÀÚ¼¼ÇÑ ³»¿ëÀº Á¦Ç°¸Å´º¾óÀ» Âü°í.
¤ýROX Reference Dye¸¦ »ç¿ëÇÏ´Â ±âÁ¾
       ROX Reference Dye (50¡¿)
              Applied Biosystems 7900HT/7300 Real-Time PCR System (Thermo Fisher Scientific)
              StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)
       ROX Reference Dye II (50¡¿)
              Applied Biosystems 7500/7500 Fast Real-Time PCR System (Thermo Fisher Scientific)
¤ýROX Reference Dye¸¦ »ç¿ëÇÏÁö ¾Ê´Â ±âÁ¾
       CronoSTAR¢â 96 Real-Time PCR System(4ch/6ch)
       Thermal Cycler Dice Real Time System ½Ã¸®Áî (Takara)
       LightCycler/LightCycler 480 system (Roche Diagnostics)
       CFX96 Real-Time PCR Detection System (Bio-Rad)
Á¦Ç° Ư¡
  • One Step RT-PCR ¹æ¹ýÀ¸·Î RNA¹ÙÀÌ·¯½º µîÀÇ Çؼ®À» ½Å¼Ó Á¤È®ÇÏ°Ô ÇÒ ¼ö ÀÖ´Ù.
  • º» Á¦Ç°Àº ´Ù¾çÇÑ ¹ÝÀÀ¾ïÁ¦¹°Áú¿¡ ³ôÀº ÀúÇ×¼ºÀ» ³ªÅ¸³»¹Ç·Î, °£ÀÌ ÇÙ»êÃßÃ⠽þàÀ» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ crude RNA »ùÇÿ¡¼­µµ Real Time RT-PCR ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù.
    £ª´Ù¸¸, °ËÃâ ºÒ´ÉÀÏ °æ¿ì³ª, º¸´Ù Á¤¹ÐÇÑ ¾ÈÁ¤µÈ Çؼ® °á°ú¸¦ ÇÊ¿ä·Î ÇÏ´Â °æ¿ì¿¡´Â ¼øµµ ³ôÀº RNA»ùÇÃÀ» ÀÌ¿ëÇÑ´Ù.
    ¹è¾ç ¼¼Æ÷³ª Á¶Á÷ »ùÇÃÀÇ °í¼øµµ total RNAÀÇ Á¶Á¦ ½Ã ±ÇÀå Á¦Ç°
    : TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
    : RNAiso Plus (Code 9108/9109)
º¸Á¸ -20¡É
¹ÝÀÀ¾ïÁ¦¹°Áú¿¡ ´ëÇÑ ³»¼º½ÇÇè ¿¹
[¹ÝÀÀÀúÇØ ¹°Áú¿¡ ´ëÇÑ ³»¼º Å×½ºÆ®]

±×¸² 1. ¹ÝÀÀ ¾ïÁ¦¹°Áú¿¡ ´ëÇÑ ³»¼º ½ÃÇè
´Ù¼¸ Á¾·ùÀÇ ¹ÝÀÀ ¾ïÁ¦¹°ÁúÀ» ¹ÝÀÀ ¿ë¾×¿¡ ÷°¡ÇÏ¿© One Step PrimeScript¢â III RT-qPCR Mix, Takara ±âÁ¸ Á¦Ç° ¹× Ÿ»ç Á¦Ç°ÀÇ ÀúÇØ ¹°Áú¿¡ ´ëÇÑ ³»¼ºÀ» Å×½ºÆ®ÇÏ¿´´Ù. One Step PrimeScript¢â III RT-qPCR Mix´Â "÷°¡ ¾øÀ½"°ú Ct°ªÀÇ Â÷ÀÌ°¡ Àû°í °¢Á¾ ¹ÝÀÀ ÀúÇع°Áú¿¡ ´ëÇÑ ³»¼ºÀÌ ³ôÀº °ÍÀ¸·Î ³ªÅ¸³µ´Ù.


±×¸² 2. Multiplex ºÐ¼®¿¡¼­ÀÇ ÀçÇö¼º
Human HeLa Cell Total RNA 1 pg~100 ngÀ» ÁÖÇüÀ¸·Î One Step PrimeScript¢â III RT-qPCR Mix¸¦ ÀÌ¿ëÇØ ACTB, GAPDH, PPIA, YWHAZ À¯ÀüÀÚÀÇ multiplex ¹ßÇö Çؼ®À» ½Ç½ÃÇÏ¿´´Ù. ¸ðµç Ÿ°Ù¿¡¼­ R2>0.99¸¦ ÃæÁ·½ÃÄ×´Ù.


±×¸² 3. Human ACTB À¯ÀüÀÚ ¹ßÇö Çؼ®¿¡¼­ÀÇ ÀçÇö¼º È®ÀÎ
Human HeLa Cell Total RNA (Code 636543) 1 pg ~1 §¶À» ÁÖÇüÀ¸·Î One Step PrimeScript¢â III RT-qPCR Mix, with UNG¸¦ ÀÌ¿ëÇÏ¿© ACTBÀ¯ÀüÀÚ ¹ßÇö Çؼ®À» 3ÀÏ°£ ³ª´©¾î ÁøÇàÇÏ¿´´Ù. ±× °á°ú, One Step PrimeScript¢â III RT-qPCR Mix, with UNGÀº ³ôÀº Á÷¼±¼º (Rsq: 1)À» °¡Áö¸ç ¾ÈÁ¤µÈ ÁõÆø È¿À² (Eff. 92.5%)¸¦ ³ªÅ¸³»¾ú´Ù.

Keyword :

Dual-Labeled Probe °ËÃâ
>
Multiplex probe qPCR ½Ã¾à (UNG Æ÷ÇÔ)
Probe qPCR Mix MultiPlus
>
´Ù¾çÇÑ »ùÇÿ¡ Àû¿ë¼ºÀÌ ³ôÀº Dual-Labeled Pro..
Probe qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)
>
½Å¼ÓÇÑ À¯ÀüÀÚ¹ßÇö Çؼ®À» À§ÇÑ 1 step qRT-PCR..
2 x all premix 1 step qRT-PCR kit
>
Premix ŸÀÔ- gDNA Á¦°Å°úÁ¤À» Ãß°¡ÇÑ qPCR Àü..
[New] ½Ã°£´ÜÃà, gDNA Á¦°Å& RT ½Ã¾à (qPCR¿ë)
>
Probe¸¦ »ç¿ëÇÑ °í°¨µµ °ËÃâ 1 step RT qPCR
One Step PrimeScript¢â RT-PCR Kit (Perfect Real Time)
>
Genomic DNA Á¦°Å°úÁ¤ÀÌ Ãß°¡µÈ qPCR¿ë cDNA ÇÕ..
gDNA Á¦°Å& RT ½Ã¾à (qPCR¿ë)
>
qPCR¿ë cDNAÇÕ¼º Premix ½Ã¾à
5x RT Master Mix (qPCR¿ë)
>
2 Step Real Time RT-PCR ¿ë ¿ªÀü»ç ½Ã¾à
RT components (qPCR¿ë)
>
Ç÷¾×, ¼Òº¯ µî »ýü»ùÇÃÀÇ direct qRT-PCR
PrimeDirect¢ç Probe RT-qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)